Engineering expression of the heavy metal transporter MerC in Saccharomyces cerevisiae for increased cadmium accumulation

Applied Microbiology and Biotechnology

Volumn 86, Issue 2, 2010, Pages 753-759

  Kiyono, Masako ^a   Miyahara, Kiyomi ^a   Sone, Yuka ^a   Pan Hou, Hidemitsu ^b   Uraguchi, Shimpei ^c   Nakamura, Ryosuke ^a   Sakabe, Kou ^a  

^a KITASATO UNIVERSITY   (Japan)

^b SETSUNAN UNIVERSITY   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

Bacterial heavy metal transport; Bioremediation; Cadmium; MerC; SNARE

Indexed keywords

BACTERIAL HEAVY METAL TRANSPORT; CADMIUM ACCUMULATIONS; CADMIUM IONS; CONTROL-CELL; ENDOPLASMIC RETICULUM; ENGINEERING EXPRESSION; FUSION PROTEINS; GREEN FLUORESCENT PROTEIN; MOLECULAR TOOLS; PLASMA MEMBRANES; SACCHAROMYCES CEREVISIAE; TARGETING MOLECULES; VACUOLAR MEMBRANES; YEAST CELL;

BACTERIOLOGY; BIODEGRADATION; BIOREMEDIATION; BIOTECHNOLOGY; CADMIUM; CADMIUM COMPOUNDS; CELL MEMBRANES; GENE EXPRESSION; POLLUTION; YEAST;

PROTEINS;

CADMIUM; FUNGAL PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROTEIN MERC; PROTEIN MERC SSO1P; PROTEIN MERC VAM3P; SNARE PROTEIN; UNCLASSIFIED DRUG;

BIOACCUMULATION; BIOREMEDIATION; CADMIUM; GENE EXPRESSION; GENETIC ENGINEERING; PROTEIN; YEAST;

ARTICLE; BIOREMEDIATION; CELL MEMBRANE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; GENE EXPRESSION; NONHUMAN; PROTEIN LOCALIZATION; SACCHAROMYCES CEREVISIAE; TONOPLAST;

CADMIUM; CELL MEMBRANE; GENE EXPRESSION; GENETIC ENGINEERING; INTRACELLULAR MEMBRANES; MEMBRANE TRANSPORT PROTEINS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

BACTERIA (MICROORGANISMS); SACCHAROMYCES CEREVISIAE;

EID: 77249140942     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2402-0     Document Type: Article

References (17)

1. MK Aalto H Ronne S Keränen 1993 Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport EMBO J 12 4095 4104 1:CAS:528:DyaK2cXntFygtQ%3D%3D
2. Amberg D, Burke D, Strathern J (2005) Methods in yeast genetics: a Cold Spring Harbor laboratory course manual, vol 2005 edition. Cold Spring Harbor Laboratory Press, New York
3. S Clemens DM Antosiewicz JM Ward DP Schachtman JI Schroeder 1998 The plant cDNA LCT1 mediates the uptake of calcium and cadmium in yeast Proc Natl Acad Sci USA 95 12043 12048 10.1073/pnas.95.20.12043 1:CAS:528: DyaK1cXmsV2htL4%3D (Pubitemid 28460536)
4. D Gietz A St Jean RA Woods RH Schiestl 1992 Improved method for high efficiency transformation of intact yeast cells Nucleic Acids Res 20 1425 10.1093/nar/20.6.1425 1:CAS:528:DyaK38XisV2mu7w%3D
5. A Malik 2004 Metal bioremediation through growing cells Environ Int 30 261 278 10.1016/j.envint.2003.08.001 1:CAS:528:DC%2BD2cXmvFGksQ%3D%3D (Pubitemid 38111128)
6. T Nagata A Nakamura T Akizawa H Pan-Hou 2009 Genetic engineering of transgenic tobacco for enhanced uptake and bioaccumulation of mercury Biol Pharm Bull 32 1491 1495 10.1248/bpb.32.1491 1:CAS:528:DC%2BD1MXhtlOqtb3I
7. CW Ostrowicz CT Meiringer C Ungermann 2008 Yeast vacuole fusion: a model system for eukaryotic endomembrane dynamics Autophagy 4 5 19 1:CAS:528:DC%2BD1cXks1Shu7w%3D
8. H Pan-Hou M Kiyono T Kawase T Omura G Endo 2001 Evaluation of ppk-specified polyphosphate as a mercury remedial tool Biol Pharm Bull 24 1423 1426 10.1248/bpb.24.1423 1:CAS:528:DC%2BD3MXosl2rsrY%3D (Pubitemid 33133760)
9. H Pan-Hou M Kiyono H Omura T Omura G Endo 2002 Polyphosphate produced in recombinant Escherichia coli confers mercury resistance FEMS Microbiol Lett 207 159 164 10.1111/j.1574-6968.2002.tb11045.x 1:CAS:528:DC%2BD38XhslWitbc%3D
10. A Rodriguez-Navarro J Ramos 1984 Dual system for potassium transport in Saccharomyces cerevisiae J Bacteriol 159 940 945 1:CAS:528:DyaL2cXls1Kru70%3D
11. + antiport activity J Biol Chem 268 12297 12302 1:CAS:528:DyaK3sXks1Ghsb0%3D
12. Y Sasaki T Minakawa A Miyazaki S Silver T Kusano 2005 Functional dissection of a mercuric ion transporter, MerC, from Acidithiobacillus ferrooxidans Biosci Biotechnol Biochem 69 1394 1402 10.1271/bbb.69.1394 1:CAS:528:DC%2BD2MXnvVSmt78%3D (Pubitemid 41185225)
13. + exchanger EMBO J 18 3973 3980 10.1093/emboj/18.14.3973 1:CAS:528:DyaK1MXkvV2isrc%3D (Pubitemid 29335850)
14. WY Song EJ Sohn E Martinoia YJ Lee YY Yang M Jasinski C Forestier I Hwang Y Lee 2003 Engineering tolerance and accumulation of lead and cadmium in transgenic plants Nat Biotechnol 21 914 919 10.1038/nbt850 1:CAS:528: DC%2BD3sXlvVKguro%3D (Pubitemid 36936198)
15. K Tanaka M Nakafuku F Tamanoi Y Kaziro K Matsumoto A Toh-e 1990 IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein Mol Cell Biol 10 4303 4313 1:CAS:528:DyaK3cXmt1Gntr8%3D
16. T Uemura T Ueda RL Ohniwa A Nakano K Takeyasu MH Sato 2004 Systematic analysis of SNARE molecules in Arabidopsis: dissection of the post-Golgi network in plant cells Cell Struct Funct 29 49 65 10.1247/csf.29.49 1:CAS:528:DC%2BD2cXntlOrurg%3D (Pubitemid 39206720)
17. Y Wada N Nakamura Y Ohsumi A Hirata 1997 Vam3p, a new member of syntaxin related protein, is required for vacuolar assembly in the yeast Saccharomyces cerevisiae J Cell Sci 110 1299 1306 1:CAS:528:DyaK2sXktF2mtbg%3D