Expression, purification and direct eletrochemistry of cytochrome P450 6A1 from the house fly, Musca domestica

Protein Expression and Purification

Volumn 71, Issue 1, 2010, Pages 74-78

  Zhang, Li ^a   Liu, Xuequn ^a   Wang, Chuntai ^a   Liu, Xinqiong ^a   Cheng, Gang ^a   Wu, Yunhua ^a  

^a National Commission for Nationalities   (China)

Author keywords

Aldrin; Cyclic voltammetry; Cytochrome P450; House fly; Insecticides

Indexed keywords

ALDRIN; CYTOCHROME P450; HYDROXYAPATITE; RECOMBINANT PROTEIN; SEPHAROSE; SEPHAROSE FAST FLOW SP;

ANIMAL; ARTICLE; CHROMATOGRAPHY; ELECTROCHEMISTRY; ENZYME ASSAY; ENZYMOLOGY; HOUSE FLY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; SPECTROSCOPY;

ALDRIN; ANIMALS; CHROMATOGRAPHY; CYTOCHROME P-450 ENZYME SYSTEM; DURAPATITE; ELECTROCHEMISTRY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ASSAYS; HOUSEFLIES; RECOMBINANT PROTEINS; SEPHAROSE; SPECTRUM ANALYSIS;

ESCHERICHIA COLI; MUSCA DOMESTICA;

EID: 77049112019     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.12.008     Document Type: Article

References (21)

1. Coon M.J., Ding X., Pernecky S.J., and Vaz A.D.N. Cytochrome P450: progress and predictions. FASEB J. 6 (1992) 669-673
2. Feyereisen R., Pratt G.E., and Hamnett A.F. Enzymic synthesis of juvenile hormone in locust corpora allata: evidence for a microsomal cytochrome P-450 linked methyl farnesoate epoxidase. Eur. J. Biochem. 118 (1981) 231-238
3. Dowd P.F., Smith C.M., and Sparks T.C. Detoxification of plants toxins by insects. Insect Biochem. 13 (1983) 453-468
4. Agosin M. In: Kerkut G.A., and Gilbert L.I. (Eds). Comprehensive Insect Physiology, Biochemistry and Pharmacology, vol. 12 (1985), Pergamon, Oxford 647-712
5. Tate L.G., Plapp F.W., and Hodgson E. Cytochrome P-450 difference spectra of microsomes from several insecticide-resistant and -susceptible strains of the housefly, Musca domestica L. Chem. Biol. Interact. 6 (1973) 237-247
6. Moldenke A.F., and Terriere L.C. Effects of imazalil on a wild-type and fungicide-resistant strain of Aspergillus nidulans. Pestic. Biochem. Physiol. 16 (1981) 222-230
7. Feyereisen R., Koener J.F., Farnsworth D., and Nebert D.W. Isolation and sequence of cDNA encoding a cytochrome P-450 from an insecticide-resistant strain of the house fly, Musca domestica. Proc. Natl. Acad. Sci. USA 86 (1989) 1465-1469
8. Andersen J.F., Utermohlen J.G., and Feyereisen R. Expression of housefly CYP6A1 and NADPH-cytochrome P450 reductase in Escherichia coli and reconstitution of an insecticide-metabolizing P450 system. Biochemistry 33 (1994) 2171-2177
9. Lei C.H., Wollenberger U., Jung C., and Scheller F.W. Clay-bridged electron transfer between cytochrome P450cam and electrode. Biochem. Biophys. Res. Commun. 268 (2000) 740-744
10. Fantuzzi A., Fairhead M., and Gilardi G. Direct electrochemistry of immobilized human cytochrome P450 2E1. J. Am. Chem. Soc. 126 (2004) 5040-5041
11. Shumyantseva V.V., Ivanov Y.D., Bistolas N., Scheller F.W., Archakov A.I., and Wollenberger U. Direct electron transfer of cytochrome P450 2B4 at electrodes modified with nonionic detergent and colloidal clay nanoparticles. Anal. Chem. 76 (2004) 6046-6052
12. Rhieu S.Y., Ludwig D.R., Siu V.S., and Palmore G.T.R. Direct electrochemistry of cytochrome P450 27B1 in surfactant films. Electrochem. Commun. 11 (2009) 1857-1860
13. Liu S.Q., Peng L., Yang X.D., Wu Y.F., and He L. Electrochemistry of cytochrome P450 enzyme on nanoparticle-containing membrane-coated electrode and its applications for drug sensing. Anal. Biochem. 375 (2008) 209-216
14. Stormo G.D., Schneider T.D., and Gold L.D. Characterization of translational initiation sites in E. coli. Nucleic Acids Res. 10 (1982) 2971-2996
15. Higuchi R., Krummel B., and Saiki R.K. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res. 16 (1988) 7351-7367
16. Omura T., and Sato R. The carbon monoxide-binding pigment of liver microsomes. J. Biol. Chem. 239 (1964) 2370-2378
17. Lowry O.H., Rosebrough N.J., Farr A.L., and Randell R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
18. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
19. Wu Y.H., Shen Q.C., and Hu S.S. Direct electrochemistry and electrocatalysis of heme-proteins in regenerated silk fibroin film. Anal. Chim. Acta 558 (2006) 179-186
20. Rusling J.F. Enzyme bioelectrochemistry in cast biomembrane-like films. Acc. Chem. Res. 31 (1998) 363-369
21. Lewis D.F.V. Cytochrome P450: Structure, Function, and Mechanism (1996), Taylor & Francis, London