Synthesis, purification and characterization of recombinant glycosylated human prolactin (G-hPRL) secreted by cycloheximide-treated CHO cells

Journal of Biotechnology

Volumn 145, Issue 4, 2010, Pages 334-340

  Heller, S R ^a   Rodrigues Goulart, H ^a   Arthuso, F S ^a   Oliveira, T L ^a   Bartolini, P ^a   Soares, C R J ^a  

^a INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES   (Brazil)

Author keywords

CHO cells; Cycloheximide; G PRL; Glycosylated human prolactin; HPRL; Human prolactin

Indexed keywords

AMINO ACIDS; BIOASSAY; BIOSYNTHESIS; CELLS; CHARACTERIZATION; CYTOLOGY; PHYSIOLOGY; PROTEINS; PURIFICATION;

CHO CELL; CYCLOHEXIMIDES; G-PRL; HPRL; HUMAN PROLACTINS;

BIOACTIVITY;

CYCLOHEXIMIDE; GLYCOSYLATED PROTEIN; PROLACTIN; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; BIOASSAY; CATION EXCHANGE; CELL CULTURE; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; GEL PERMEATION CHROMATOGRAPHY; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO STUDY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEIN SYNTHESIS; WESTERN BLOTTING;

EUKARYOTA;

EID: 77049096686     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.12.019     Document Type: Article

References (25)

1. Bannerman D.D., Tupper J.C., Erwert R.D., Winn R.K., Harlan J.M. Divergence of bacterial lipopolysaccharide pro-apoptotic signaling downstream of IRAK-1. J. Biol. Chem. 2002, 277:8048-8053.
2. Butnev V.Y., Gotschall R.R., Baker V.L., Moore W.T., Gout P.W., Bousfield G.R. Glycosylated equine prolactin and its carbohydrate moiety. J. Protein Chem. 1996, 15:413-426.
3. Dalmora S., Oliveira J.E., Affonso R., Gimbo E., Ribela M.T.C.P., Bartolini P. Analysis of recombinant human growth hormone directly in osmotic shock fluids. J. Chromatogr. A 1997, 782:199-210.
4. Freeman M.E., Kanyicska B., Lerant A., Nagy G. Prolactin: structure, function, and regulation of secretion. Physiol. Rev. 2000, 80:1523-1631.
5. Glezer A., Soares C.R.J., Vieira J.G., Giannella-Neto D., Ribela M.T.C.P., Goffin V., Bronstein M.D. Human macroprolactin displays low biological activity via its homologous receptor in a new sensitive bioassay. J. Clin. Endocrinol. Metab. 2006, 91:1048-1055.
6. Goffin V., Binart N., Touraine P., Kelly P.A. Prolactin: the new biology of an old hormone. Annu. Rev. Physiol. 2002, 64:47-67.
7. Hoffmann T., Gunz G., Brue T., Jaquet P., Ronin C. Prolactin isoforms secreted by human prolactinomas. Horm. Res. 1992, 38:164-170.
8. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
9. Lewis U.J., Singh R.N., Sinha Y.N., Vanderlaan W.P. Glycosylated human prolactin. Endocrinology 1985, 116:359-363.
10. Lewis U.J., Singh R.N., Lewis L.J. Two forms of glycosylated human prolactin have different pigeon crop sac-stimulating activities. Endocrinology 1989, 124:1558-1563.
11. Oliveira J.E., Damiani R., Vorauer-Uhl K., Bartolini P., Ribela M.T.C.P. Influence of a reduced CO2 environment on the secretion yield, potency and N-glycan structures of recombinant thyrotropin from CHO. Cells Mol. Biotechnol. 2008, 39:159-166.
12. Pellegrini I., Gunz G., Ronin C., Fenouillet E., Peyrat J.P., Delori P., Jaquet P. Polymorphism of prolactin secreted by human prolactinoma cells: immunological, receptor binding, and biological properties of the glycosylated and nonglycosylated forms. Endocrinology 1988, 122:2667-2674.
13. Price A.E., Logvinenko K.B., Higgins E.A., Cole E.S., Richards S.M. Studies on the microheterogeneity and in vitro activity of glycosylated and nonglycosylated recombinant human prolactin separated using a novel purification process. Endocrinology 1995, 136:4827-4833.
14. Rafferty B., Rigsby P., Gaines-Das R.E. 2001. Draft report of an international collaborative study of proposed WHO reference reagents for rDNA-derived prolactin and its glycosylated and non-glycosylated componentes. WHO TRS (Technical Report Series). 52, N° 924, Geneva.
15. Ribela M.T.C.P., Bianco A.C., Bartolini P. The use of recombinant human thyrotropin produced by Chinese hamster ovary cells for the preparation of immunoassay reagents. J. Clin. Endocrinol. Metab. 1996, 81:249-256.
16. Shelikoff M., Sinskey A.J., Stephanopoulos G. The effect of protein synthesis inhibitors on the glycosylation site occupancy of recombinant human prolactin. Cytotechnology 1994, 15:195-208.
17. Shelikoff M., Sinskey A.J., Stephanopoulos G. A modeling framework for the study of protein glycosylation. Biotechnol. Bioeng. 1996, 50:73-90.
18. Sinha Y.N., Depaolo L.V., Haro L.S., Singh R.N., Jacobsen B.P., Scott K.E., Lewis U.J. Isolation and biochemical properties of four forms of glycosylated porcine prolactin. Mol. Cell Endocrinol. 1991, 80:203-213.
19. Sinha Y.N. Structural variants of prolactin: occurrence and physiological significance. Endocr. Rev. 1995, 16:354-369.
20. Soares C.R.J., Morganti L., Miloux B., Lupker J.H., Ferrara P., Bartolini P. High-level synthesis of human prolactin in Chinese hamster ovary cells. Biotechnol. Appl. Biochem. 2000, 32:127-135.
21. Soares C.R.J., Camargo I.M., Morganti L., Gimbo E., Oliveira J.E., Legoux R., Ferrara P., Bartolini P. Reversed-phase high-performance liquid chromatography method for the determination of prolactin in bacterial extracts and in its purified form. J. Chromatogr. A 2002, 955:229-236.
22. Soares C.R.J., Glezer A., Okazaki K., Ueda E.K.M., Heller S.R., Walker A.M., Goffin V., Bartolini P. Physico-chemical and biological characterizations of two human prolactin analogs exhibiting controversial bioactivity, synthesized in Chinese hamster ovary (CHO) cells. Protein Expr. Purif. 2006, 48:182-194.
23. Soares C.R.J., Ueda E.K.M., Oliveira T.L., Gomide F.I., Heller S.R., Bartolini P. Distinct human prolactin (hPRL) and growth hormone (hGH) behavior under bacteriophage lambda PL promoter control: temperature plays a major role in protein yields. J. Biotechnol. 2008, 133:27-35.
24. Tanaka T., Shiu R.P., Gout P.W., Beer C.T., Noble R.L., Friesen H.G. A new sensitive and specific bioassay for lactogenic hormones: measurement of prolactin and growth hormone in human serum. J. Clin. Endocrinol. Metab. 1980, 51:1058-1063.
25. Young K.H., Kraeling R.R., Bazer F.W. Effect of pregnancy and exogenous ovarian steroids on endometrial prolactin receptor ontogeny and uterine secretory response in pigs. Biol. Reprod. 1990, 43:592-599.