Control of cellular adhesiveness in an alginate-based hydrogel by varying peroxidase and H2O2 concentrations during gelation

Acta Biomaterialia

Volumn 6, Issue 4, 2010, Pages 1446-1452

  Sakai, S ^a   Hirose, K ^a   Moriyama, K ^a   Kawakami, K ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Alginate; Alginate lyase; Cellular adhesion; Enzymatic gelation; Peroxidase

Indexed keywords

ADHESION; ALGINATE; CELL CULTURE; CELLS; CROSSLINKING; HYDROGELS; SOLUTIONS;

ALGINATE LYASE; CELL SHEET; CELLULAR ADHESION; CELLULARS; ENZYMATIC GELATION; HORSE-RADISH PEROXIDASE; OXIDATIVE CROSSLINKING; PEROXIDASE; PHENOLIC HYDROXYL; PHENOLIC HYDROXYL GROUP;

GELATION;

ALGINIC ACID; HORSERADISH PEROXIDASE; HYDROGEN PEROXIDE;

ANIMAL CELL; AQUEOUS SOLUTION; ARTICLE; CELL ADHESION; CELL CULTURE; CELL PROLIFERATION; CELL STRAIN L 929; COMPRESSION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CROSS LINKING; GELATION; HYDROGEL; NONHUMAN; PRIORITY JOURNAL;

ARMORACIA RUSTICANA;

EID: 76949083867     PISSN: 17427061     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.actbio.2009.10.004     Document Type: Article

References (19)

1. Lee F., Chung J.E., and Kurisawa M. An injectable hyaluronic acid-tyramine hydrogel system for protein delivery. J Control Release 134 (2009) 186-193
2. Kurisawa M., Chung J.E., Yang Y.Y., Gao S.J., and Uyama H. Injectable biodegradable hydrogels composed of hyaluronic acid-tyramine conjugates for drug delivery and tissue engineering. Chem Commun 34 (2005) 4312-4314
3. Sakai S., Hirose K., Taguchi K., Ogushi Y., and Kawakami K. An injectable, in situ enzymatically gellable, gelatin derivative for drug delivery and tissue engineering. Biomaterials 30 (2009) 3371-3377
4. Westhaus E., and Messersmith P.B. Triggered release of calcium from lipid vesicles: a bioinspired strategy for rapid gelation of polysaccharide and protein hydrogels. Biomaterials 22 (2001) 453-462
5. Schmidt A., Schumacher J.T., Reichelt J., Hecht H.J., and Bilitewski U. Mechanistic and molecular investigations on stabilization of horseradish peroxidase C. Anal Chem 74 (2002) 3037-3045
6. Jin R., Hiemstra C., Zhong Z., and Feijen J. Enzyme-mediated fast in situ formation of hydrogels from dextran-tyramine conjugates. Biomaterials 28 (2007) 2791-2800
7. Sakai S., and Kawakami K. Synthesis and characterization of both ionically and enzymatically crosslinkable alginate. Acta Biomater 3 (2007) 495-501
8. Ogushi Y., Sakai S., and Kawakami K. Synthesis of enzymatically-gellable carboxymethylcellulose for biomedical applications. J Biosci Bioeng 104 (2007) 30-33
9. Sakai S., Yamada Y., Zenke T., and Kawakami K. Novel chitosan derivative soluble at neutral pH and in-situ gellable via peroxidase-catalyzed enzymatic reaction. J Mater Chem 19 (2009) 230-235
10. Lee F., Chung J.E., and Kurisawa M. An injectable enzymatically crosslinked hyaluronic acid-tyramine hydrogel system with independent tuning of mechanical strength and gelation rate. Soft Matter 4 (2008) 880-887
11. Ogushi Y., Sakai S., and Kawakami K. Phenolic hydroxy groups incorporated for the peroxidase-catalyzed gelation of a carboxymethylcellulose support: cellular adhesion and proliferation. Macromol Biosci 9 (2009) 262-267
12. Velzenberger E., Vayssade M., Legeay G., and Nagel M.D. Study of cell behavior on cellulose anti-adhesive substratum. Cellulose 15 (2008) 347-357
13. Sakai S., Ono T., Ijima H., and Kawakami K. Modification of porous aminopropyl-silicate microcapsule membrane by electrically-bonded external anionic polymers. J Biomater Sci Polym Ed 14 (2003) 643-652
14. Sakai S., Ogushi Y., and Kawakami K. Enzymatically crosslinked carboxymethylcellulose-tyramine conjugate hydrogel: cellular adhesiveness and feasibility for cell sheet technology. Acta Biomater 5 (2009) 554-559
15. Augst A.D., Kong H.J., and Mooney D.J. Alginate hydrogels as biomaterials. Macromol Biosci 6 (2006) 623-633
16. Smidsrod O., and Skjak-Braek G. Alginate as immobilization matrix for cells. Trends Biotechnol 8 (1990) 71-78
17. Rowley J.A., Madlambayan G., and Mooney D.J. Alginate hydrogels as synthetic extracellular matrix materials. Biomaterials 20 (1999) 45-53
18. Bernkop Schnurch A., Kast C.E., and Richter M.F. Improvement in the mucoadhesive properties of alginate by the covalent attachment of cysteine. J Control Release 71 (2001) 277-285
19. Yang J., Yamato M., Shimizu T., Sekine H., Ohashi K., Kanzaki M., et al. Reconstruction of functional tissues with cell sheet engineering. Biomaterials 28 (2007) 5033-5043