Exploiting the intracellular compartmentalization characteristics of the S. cerevisiae host cell for enhancing primary purification of lipid-envelope virus-like particles

Biotechnology Progress

Volumn 26, Issue 1, 2010, Pages 26-33

  Kee, Gaik Sui ^a   Jin, Jing ^a   Balasundaram, Bangaru ^a   Bracewell, Daniel G ^a   Pujar, Narahari S ^b   Titchener Hooker, Nigel J ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Compartmentalization; Lipid envelope virus like particles; Purification; Saccharomyces cerevisiae; Selective product recovery

Indexed keywords

CELL DISRUPTION; CELL HOMOGENATE; CLEANER PROCESS; COEXTRACTION; CYTOSOLIC; ENDOPLASMIC RETICULUM; HEPATITIS B SURFACE ANTIGEN; HOST CELLS; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; INTRACELLULAR COMPARTMENTALIZATION; LIFE SPAN; PRESSURE CONDITIONS; PRODUCT RECOVERY; PRODUCT STREAMS; RECOVERY PROCESS; S.CEREVISIAE; SACCHAROMYCES CEREVISIAE; SELECTIVE PRODUCT RECOVERY; SHEAR CONDITIONS; SOLIDS FRACTION; VIRUS-LIKE PARTICLES; YEAST PROTEINS;

CENTRIFUGATION; CHROMATOGRAPHIC ANALYSIS; CONTAMINATION; DETERGENTS; HYDRAULICS; HYDROPHOBICITY; LIPIDS; NUCLEIC ACIDS; PURIFICATION; VIRUSES; YEAST;

HYDROPHOBIC CHROMATOGRAPHY;

DETERGENT; HEPATITIS B SURFACE ANTIGEN; S ENVELOPE PROTEIN, HEPATITIS B VIRUS; VIRUS ENVELOPE PROTEIN;

ARTICLE; CELL COMPARTMENTALIZATION; CELL CULTURE; CHEMISTRY; CYTOLOGY; ENDOPLASMIC RETICULUM; FRACTIONATION; GENETICS; INTRACELLULAR SPACE; ISOLATION AND PURIFICATION; METABOLISM; SACCHAROMYCES CEREVISIAE; VIROLOGY;

CELL COMPARTMENTATION; CELLS, CULTURED; CHEMICAL FRACTIONATION; DETERGENTS; ENDOPLASMIC RETICULUM; HEPATITIS B SURFACE ANTIGENS; INTRACELLULAR SPACE; SACCHAROMYCES CEREVISIAE; VIRAL ENVELOPE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 76849096486     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.307     Document Type: Article

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