Methodological assessment of acid-etching for visualizing the osteocyte lacunar-canalicular networks using scanning electron microscopy

Microscopy Research and Technique

Volumn 73, Issue 3, 2010, Pages 182-186

  Kubek, Daniel J ^a   Gattone II, Vincent H ^a   Allen, Matthew R ^a  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Acid etching; Bone; Methodology; Osteocytes; SEM

Indexed keywords

BONE; ETCHING; PHOSPHORIC ACID; VISUALIZATION;

'LACUNA'; ACID-ETCHING; BONE CELLS; CELLULAR NETWORK; ETCHING MEDIA; MATRIX; METHODOLOGY; MINERALISED MATRIX; OSTEOCYTES; QUALITATIVE EVALUATIONS;

SCANNING ELECTRON MICROSCOPY;

LACUNA;

EID: 76749167992     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20772     Document Type: Article

References (17)

1. Abe K, Hashizume H, Ushiki T. 1992. An EDTA-KOH method to expose bone cells for scanning electron microscopy. J Electron Microsc (Tokyo) 41:113-115.
2. Aguirre JI, Plotkin LI, Stewart SA, Weinstein RS, Parfitt AM, Manolagas SC, Bellido T. 2006. Osteocyte apoptosis is induced by weightlessness in mice and precedes osteoclast recruitment and bone loss. J Bone Miner Res 21:605-615.
3. Allen MR, Burr DB. 2008. Mandible matrix necrosis in beagle dogs after 3 years of daily oral bisphosphonate treatment. J Oral Maxillofac Surg 66:987-994.
4. Allen MR, Iwata K, Phipps R, Burr DB. 2006. Alterations in canine vertebral bone turnover, microdamage accumulation, and biomechanical properties following 1-year treatment with clinical treatment doses of risedronate or alendronate. Bone 39:872-879.
5. Bonewald LF. 2007. Osteocytes as dynamic multifunctional cells. Ann NYAcad Sci 1116:281-290.
6. Curtis TA, Ashrafi SH, Weber DF. 1985. Canalicular communication in the cortices of human long bones. Anat Rec 212:336-344.
7. Ejiri S, Ozawa H. 1982. Scanning electron microscopic observations of rat tibia using the HCl-collagenase method. Arch Histol Jpn 45:399-404.
8. Feng JQ, Ward LM, Liu S, Lu Y, Xie Y, Yuan B, Yu X, Rauch F, Davis SI, Zhang S, Rios H, Drezner MK, Quarles LD, Bonewald LF, White KE. 2006. Loss of DMP1 causes rickets and osteomalacia and identifies a role for osteocytes in mineral metabolism. Nat Genet 38:1310-1315.
9. Frost HM. 1960. Micropetrosis. J Bone Joint Surg Am A 42:144-150.
10. Gross TS, Akeno N, Clemens TL, Komarova S, Srinivasan S, Weimer DA, Mayorov S. 2001. Selected contribution: Osteocytes upregulate HIF-1a in response to acute disuse and oxygen deprivation. J Appl Physiol 90:2514-2519.
11. Inoue K, Mikuni-Takagaki Y, Oikawa K, Itoh T, Inada M, Noguchi T, Park JS, Onodera T, Krane SM, Noda M, Itohara S. 2006. A crucial role for matrix metalloproteinase 2 in osteocytic canalicular formation and bone metabolism. J Biol Chem 281:33814-33824.
12. Marotti G, Remaggi F, Zaffe D. 1985. Quantitative investigation on osteocyte canaliculi in human compact and spongy bone. Bone 6:335-337.
13. O'Brien CA, Jia D, Plotkin LI, Bellido T, Powers CC, Stewart SA, Manolagas SC, Weinstein RS. 2004. Glucocorticoids act directly on osteoblasts and osteocytes to induce their apoptosis and reduce bone formation and strength. Endocrinology 145:1835-1841.
14. Okada S, Yoshida S, Ashrafi SH, Schraufnagel DE. 2002. The canalicular structure of compact bone in the rat at different ages. Microsc Microanal 8:104-115.
15. Tate ML, Adamson JR, Tami AE, Bauer TW. 2004. The osteocyte. Int J Biochem Cell Biol 36:1-8.
16. Turner CH, Robling AG, Duncan RL, Burr DB. 2002. Do bone cells behave like a neuronal network? Calcif Tissue Int 70:435-442.
17. Verborgt O, Gibson GJ, Schaffler MB. 2000. Loss of osteocyte integrity in association with microdamage and bone remodeling after fatigue in vivo. J Bone Miner Res 15:60-67.