Improved preparation and detection of cytochrome P450 isoforms using MS methods

Proteomics

Volumn 10, Issue 2, 2010, Pages 327-331

  Sutton, Chris W ^a   Sutherland, Mark ^a   Shnyder, Steve ^a   Patterson, Laurence H ^a  

^a UNIVERSITY OF BRADFORD   (United Kingdom)

Author keywords

Animal proteomics; Cytochrome P450; LC MALDI MS; Liver; Microsomes; Mouse

Indexed keywords

CHOLESTANETRIOL 26 MONOOXYGENASE; CYTOCHROME P450; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; CYTOCHROME P450 2A12; CYTOCHROME P450 2A4; CYTOCHROME P450 2A5; CYTOCHROME P450 2B9; CYTOCHROME P450 2C29; CYTOCHROME P450 2C37; CYTOCHROME P450 2C39; CYTOCHROME P450 2C40; CYTOCHROME P450 2C50; CYTOCHROME P450 2C54; CYTOCHROME P450 2C70; CYTOCHROME P450 2D10; CYTOCHROME P450 2D26; CYTOCHROME P450 2D9; CYTOCHROME P450 2E1; CYTOCHROME P450 2F2; CYTOCHROME P450 2J5; CYTOCHROME P450 3A11; CYTOCHROME P450 3A13; CYTOCHROME P450 3A25; CYTOCHROME P450 3A41; CYTOCHROME P450 4A14; CYTOCHROME P450 4F14; CYTOCHROME P450 8B1; MICROSOME ENZYME; UNCLASSIFIED DRUG; UREA;

ANIMAL CELL; ARTICLE; CHO CELL; DATA BASE; DRUG METABOLISM; GENETIC TRANSFECTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIVER; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEOMICS; SEQUENCE ANALYSIS; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; CYTOCHROME P-450 ENZYME SYSTEM; DATABASES, PROTEIN; HUMANS; ISOENZYMES; MICE; MOLECULAR SEQUENCE DATA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TANDEM MASS SPECTROMETRY;

ANIMALIA;

EID: 76749167149     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.200900489     Document Type: Article

References (8)

1. Nelson, D. R., Zeldin, D. C., Hoffman, S. M., Maltais, L. J. et al., Comparison of cytochrome P450 (CYP) genes from the mouse and human genomes, including nomenclature recommendations for genes, pseudogenes and alternativesplice variants. Pharmacogenetics 2004, 14, 1-18.
2. Jenkins, R. E., Kitteringham, N. R., Hunter, C. L., Webb, S. et al., Relative and absolute quantitative expression profiling of cytochromes P450 using isotope-coded affinity tags. Proteomics 2006, 6, 1934-1947.
3. Alterman, M. A., Kornilayev, B., Duzhak, T., Yakovlev, D., Quantitative analysis of cytochrome p450 isozymes by means of unique isozyme-specific tryptic peptides: a proteomic approach. Drug Metab. Dispos. 2005, 33, 1399-1407.
4. Nisar, S., Lane, C. S., Wilderspin, A. F., Welham, K. J. et al., A proteomic approach to the identification of cytochrome P450 isoforms in male and female rat liver by nanoscale liquid chromatography-electrospray ionization-tandem mass spectrometry. Drug Metab. Dispos. 2004, 32, 382-386.
5. Lane, C. S., Nisar, S., Griffiths, W. J., Fuller, B. J. et al., Identification of cytochrome P450 enzymes in human colorectal metastases and the surrounding liver: a proteomic approach. Eur. J. Cancer 2004, 40, 2127-2134.
6. Redlich, G., Zanger, U. M., Riedmaier, S., Bache, N. et al., Distinction between human cytochrome P450 (CYP) isoforms and identification of new phosphorylation sites by mass spectrometry. J. Proteome Res. 2008, 7, 4678-4688.
7. Lane, C. S., Wang, Y., Betts, R., Griffiths, W. J., Patterson, L. H., Comparative cytochrome P450 proteomics in the livers of immunodeficient mice using 18O stable isotope labeling. Mol. Cell. Proteomics 2007, 6, 953-962.
8. Drahushuk, A. T., McGarrigle, B. P., Larsen, K. E., Stegeman, J. J., Olson, J. R., Detection of CYP1A1 protein in human liver and induction by TCDD in precision-cut liver slices incubated in dynamic organ culture. Carcinogenesis 1998, 19, 1361-1368.