High throughput, non-invasive and dynamic toxicity screening on adherent cells using respiratory measurements

Toxicology in Vitro

Volumn 24, Issue 2, 2010, Pages 686-694

  Beckers, Simone ^a   Noor, Fozia ^a   Müller Vieira, Ursula ^b   Mayer, Manuela ^b   Strigun, Alexander ^b   Heinzle, Elmar ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b Pharmacelsus GmbH   (Germany)

Author keywords

Cytotoxicity; Luminescence decay time; Oxygen sensor; Respiration; Screening; Time resolved oxygen sensing

Indexed keywords

AMIODARONE; CARBON DIOXIDE; CLOZAPINE; DICLOFENAC; GLYCEROL; METHOTREXATE; OXYGEN; RIFAMPICIN; SULFORHODAMINE B; TACRINE; TROGLITAZONE; VERAPAMIL;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL ASSAY; CELL DEATH; CELL RESPIRATION; CELL STRAIN HEPG2; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; HUMIDIFIER; INCUBATION TIME; INCUBATOR; INTERMETHOD COMPARISON; LC 50; LIVER CELL; LIVER TOXICITY; LUMINESCENCE; MALE; MATHEMATICAL ANALYSIS; NON INVASIVE MEASUREMENT; NONHUMAN; RAT; TIME; TOXICITY TESTING; TOXICOKINETICS;

ANIMALS; BIOLOGICAL ASSAY; CELL ADHESION; CELL LINE, TUMOR; CYTOTOXINS; DRUG EVALUATION, PRECLINICAL; HEPATOCYTES; HUMANS; MALE; OXYGEN; OXYGEN CONSUMPTION; PHARMACOKINETICS; RATS; RATS, WISTAR; TOXICITY TESTS;

RATTUS;

EID: 76749146181     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2009.04.018     Document Type: Article

References (57)

1. Arnheiter H. Primary monolayer-culture of adult-mouse hepatocytes - model for the study of hepatotropic viruses. Archives of Virology 63 1 (1980) 11-22
2. Bennett C.L., Nebeker J.R., Yarnold P.R., Tigue C.C., Dorr D.A., McKoy J.M., Edwards B.J., Hurdle J.F., West D.P., Lau D.T., Angelotta C., Weitzman S.A., Belknap S.M., Djulbegovic B., Tallman M.S., Kuzel T.M., Benson A.B., Evens A., Trifilio S.M., Courtney D.M., and Raisch D.W. Evaluation of serious adverse drug reactions: a proactive pharmacovigilance program (RADAR) vs safety activities conducted by the Food and Drug Administration and pharmaceutical manufacturers. Archives of Internal Medicine 167 10 (2007) 1041-1049
3. Bonarius H.P.J., Degooijer C.D., Tramper J., and Schmid G. Determination of the respiration quotient in mammalian-cell culture in bicarbonate buffered media. Biotechnology and Bioengineering 45 6 (1995) 524-535
4. Bort R., Ponsoda X., Jover R., Gomez-Lechon M.J., and Castell J.V. Diclofenac toxicity to hepatocytes: a role for drug metabolism in cell toxicity. Journal of Pharmacology and Experimental Therapeutics 288 1 (1999) 65-72
5. Bova M.P., Tam D., McMahon G., and Mattson M.N. Troglitazone induces a rapid drop of mitochondrial membrane potential in liver HepG2 cells. Toxicology Letters 155 1 (2005) 41-50
6. Brandon E.F., Meijerman I., Klijn J.S., den Arend D., Sparidans R.W., Lazaro L.L., Beijnen J.H., and Schellens J.H. In-vitro cytotoxicity of ET-743 (Trabectedin, Yondelis), a marine anti-cancer drug, in the Hep G2 cell line: influence of cytochrome P450 and phase II inhibition, and cytochrome P450 induction. Anticancer Drugs 16 9 (2005) 935-943
7. Carraway E.R., and Demas J.N. Luminescence quenching mechanism for microheterogeneous systems. Analytical Chemistry 63 4 (1991) 332-336
8. Castell J.V., Gomez-Lechon M.J., Ponsoda X., and Bort R. The use of cultured hepatocytes to investigate the mechanisms of drug hepatotoxicity. Cell Biology and Toxicology 13 4-5 (1997) 331-338
9. Dambach D.M., Andrews B.A., and Moulin F. New technologies and screening strategies for hepatotoxicity: use of in vitro models. Toxicologic Pathology 33 1 (2005) 17-26
10. Deshpande R.R., and Heinzle E. On-line oxygen uptake rate and culture viability measurement of animal cell culture using microplates with integrated oxygen sensors. Biotechnology Letters 26 9 (2004) 763-767
11. Deshpande R.R., Koch-Kirsch Y., Maas R., John G.T., Krause C., and Heinzle E. Microplates with integrated oxygen sensors for kinetic cell respiration measurement and cytotoxicity testing in primary and secondary cell lines. Assay and Drug Development Technologies 3 3 (2005) 299-307
12. Deshpande R.R., Wittmann C., and Heinzle E. Microplates with integrated oxygen sensing for medium optimization in animal cell culture. Cytotechnology 46 1 (2004) 1-8
13. Gomez-Lechon M.J., Ponsoda X., Bort R., and Castell J.V. The use of cultured hepatocytes to investigate the metabolism of drugs and mechanisms of drug hepatotoxicity. Alternatives to Laboratory Animals 29 3 (2001) 225-231
14. Guillouzo A. Liver cell models in in vitro toxicology. Environmental Health Perspectives 106 Suppl. 2 (1998) 511-532
15. Guo L., Zhang L., Sun Y., Muskhelishvili L., Blann E., Dial S., Shi L., Schroth G., and Dragan Y.P. Differences in hepatotoxicity and gene expression profiles by anti-diabetic PPAR gamma agonists on rat primary hepatocytes and human HepG2 cells. Molecular Diversities 10 3 (2006) 349-360
16. Hewitt N.J., Lloyd S., Hayden M., Butler R., Sakai Y., Springer R., Fackett A., and Li A.P. Correlation between troglitazone cytotoxicity and drug metabolic enzyme activities in cryopreserved human hepatocytes. Chemico-Biological Interactions 142 1-2 (2002) 73-82
17. Huber C., Klimant I., Krause C., Werner T., and Wolfbeis O.S. Nitrate-selective optical sensor applying a lipophilic fluorescent potential-sensitive dye. Analytica Chimica Acta 449 1-2 (2001) 81-93
18. Hynes J., Floyd S., Soini A.E., O'Connor R., and Papkovsky D.B. Fluorescence-based cell viability screening assays using water-soluble oxygen probes. Journal of Biomolecular Screening 8 3 (2003) 264-272
19. Hynes J., Marroquin L.D., Ogurtsov V.I., Christiansen K.N., Stevens G.J., Papkovsky D.B., and Will Y. Investigation of drug-induced mitochondrial toxicity using fluorescence-based oxygen-sensitive probes. Toxicological Sciences 92 1 (2006) 186-200
20. Kanebratt K.P., and Andersson T.B. Evaluation of HepaRG cells as an in vitro model for human drug metabolism studies. Drug Metabolism and Disposition 36 7 (2008) 1444-1452
21. Kaufmann P., Torok M., Hanni A., Roberts P., Gasser R., and Krahenbuhl S. Mechanisms of benzarone and benzbromarone-induced hepatic toxicity. Hepatology 41 4 (2005) 925-935
22. Kensy F., John G.T., Hofmann B., and Buchs J. Characterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter plates. Bioprocess and Biosystems Engineering 28 2 (2005) 75-81
23. Klimant I., and Wolfbeis O.S. Oxygen-sensitive luminescent materials based on silicone-soluble ruthenium diimine complexes. Analytical Chemistry 67 18 (1995) 3160-3166
24. Kocincova A.S., Nagl S., Arain S., Krause C., Borisov S.M., Arnold M., and Wolfbeis O.S. Multiplex bacterial growth monitoring in 24-well microplates using a dual optical sensor for dissolved oxygen and pH. Biotechnology and Bioengineering 100 3 (2008) 430-438
25. Kostrubsky V.E., Sinclair J.F., Ramachandran V., Venkataramanan R., Wen Y.H., Kindt E., Galchev V., Rose K., Sinz M., and Strom S.C. The role of conjugation in hepatotoxicity of troglitazone in human and porcine hepatocyte cultures. Drug Metabolism and Disposition 28 10 (2000) 1192-1197
26. Kumar S., Wittmann C., and Heinzle E. Minibioreactors. Biotechnology Letters 26 1 (2004) 1-10
27. Lim M.S., Lim P.L., Gupta R., and Boelsterli U.A. Critical role of free cytosolic calcium, but not uncoupling, in mitochondrial permeability transition and cell death induced by diclofenac oxidative metabolites in immortalized human hepatocytes. Toxicology and Applied Pharmacology 217 3 (2006) 322-331
28. Loi C.M., Young M., Randinitis E., Vassos A., and Koup J.R. Clinical pharmacokinetics of troglitazone. Clinical Pharmacokinetics 37 2 (1999) 91-104
29. Martin A., and Clynes M. Comparison of 5 microplate colorimetric assays for invitro cytotoxicity testing and cell-proliferation assays. Cytotechnology 11 1 (1993) 49-58
30. Masubuchi Y. Metabolic and non-metabolic factors determining troglitazone hepatotoxicity: a review. Drug Metabolism and Pharmacokinetics 21 5 (2006) 347-356
31. Masubuchi Y., Yamada S., and Horie T. Possible mechanism of hepatocyte injury induced by diphenylamine and its structurally related nonsteroidal anti-inflammatory drugs. Journal of Pharmacology and Experimental Therapeutics 292 3 (2000) 982-987
32. McCarthy T.C., Pollak P.T., Hanniman E.A., and Sinal C.J. Disruption of hepatic lipid homeostasis in mice after amiodarone treatment is associated with peroxisome proliferator-activated receptor-alpha target gene activation. Journal of Pharmacology and Experimental Therapeutics 311 3 (2004) 864-873
33. Morris C., Griffiths J.B., Warburton S., West C.M.L., Al-Rubeai M., Clarke J.B., Simione F., and Doyle A. Core techniques. In: Doyle A., and Griffiths J.B. (Eds). Mammalian Cell Culture: Essential Techniques (1997), J. Wiley, Cambridge 47-102
34. Noor, F., Niklas, J., Muller-Vieira, U., Heinzle, E., 2009. An integrated approach to improved toxicity prediction for the safety assessment during preclinical drug development using Hep G2 cells. Toxicology and Applied Pharmacology, doi:10.1016/j.taap.2009.03.011.
35. Nussler A.K., Wang A., Neuhaus P., Fischer J., Yuan J., Liu L., Zeilinger K., Gerlach J., Arnold P.J., and Albrecht W. The suitability of hepatocyte culture models to study various aspects of drug metabolism. Altex 18 2 (2001) 91-101
36. O'Brien P.J., Irwin W., Diaz D., Howard-Cofield E., Krejsa C.M., Slaughter M.R., Gao B., Kaludercic N., Angeline A., Bernardi P., Brain P., and Hougham C. High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening. Archives of Toxicology 80 9 (2006) 580-604
37. O'Riordan T.C., Buckley D., Ogurtsov V., O'Connor R., and Papkovsky D.B. A cell viability assay based on monitoring respiration by optical oxygen sensing. Analytical Biochemistry 278 2 (2000) 221-227
38. Papkovsky D.B., Hynes J., and Will Y. Respirometric screening technology for ADME-Tox studies. Expert Opinion on Drug Metabolism & Toxicology 2 2 (2006) 313-323
39. Rubinstein L.V., Shoemaker R.H., Paull K.D., Simon R.M., Tosini S., Skehan P., Scudiero D.A., Monks A., and Boyd M.R. Comparison of invitro anticancer-drug-screening data generated with a tetrazolium assay versus a protein assay against a diverse panel of human tumor-cell lines. Journal of the National Cancer Institute 82 13 (1990) 1113-1118
40. Seglen P.O. Preparation of isolated rat liver cells. Methods in Cell Biology 13 (1976) 29-83
41. Skehan P., Storeng R., Scudiero D., Monks A., McMahon J., Vistica D., Warren J.T., Bokesch H., Kenney S., and Boyd M.R. New colorimetric cytotoxicity assay for anticancer-drug screening. Journal of National Cancer Institute 82 13 (1990) 1107-1112
42. Spaniol M., Bracher R., Ha H.R., Follath F., and Krahenbuhl S. Toxicity of amiodarone and amiodarone analogues on isolated rat liver mitochondria. Journal of Hepatology 35 5 (2001) 628-636
43. Tang W., Stearns R.A., Bandiera S.M., Zhang Y., Raab C., Braun M.P., Dean D.C., Pang J., Leung K.H., Doss G.A., Strauss J.R., Kwei G.Y., Rushmore T.H., Chiu S.H., and Baillie T.A. Studies on cytochrome P-450-mediated bioactivation of diclofenac in rats and in human hepatocytes: identification of glutathione conjugated metabolites. Drug Metabolism and Disposition 27 3 (1999) 365-372
44. Thibault N., Claude J.R., and Ballet F. Actin filament alteration as a potential marker for cholestasis: a study in isolated rat hepatocyte couplets. Toxicology 73 3 (1992) 269-279
45. Tirmenstein M.A., Hu C.X., Gales T.L., Maleeff B.E., Narayanan P.K., Kurali E., Hart T.K., Thomas H.C., and Schwartz L.W. Effects of troglitazone on HepG2 viability and mitochondrial function. Toxicological Sciences 69 1 (2002) 131-138
46. Toyoda Y., Tsuchida A., Iwami E., and Miwa I. Toxic effect of troglitazone on cultured rat hepatocytes. Life Sciences 68 16 (2001) 1867-1876
47. Uehara T., Kiyosawa N., Shimizu T., Omura K., Hirode M., Imazawa T., Mizukawa Y., Ono A., Miyagishima T., Nagao T., and Urushidani T. Species-specific differences in coumarin-induced hepatotoxicity as an example toxicogenomics-based approach to assessing risk of toxicity to humans. Human and Experimental Toxicology 27 1 (2008) 23-35
48. Vesey D.A., Cunningham J.M., Selden A.C., Woodman A.C., and Hodgson H.J.F. Dimethyl-sulfoxide induces a reduced growth-rate, altered cell morphology and increased epidermal-growth-factor binding in Hep G2 cells. Biochemical Journal 277 (1991) 773-777
49. Viau C.J., Curren R.D., and Wallace K. Cytotoxicity of tacrine and velnacrine metabolites in cultured rat, dog and human hepatocytes. Drug and Chemical Toxicology 16 3 (1993) 227-239
50. Vichai V., and Kirtikara K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nature Protocols 1 3 (2006) 1112-1116
51. Waldhauser K.M., Torok M., Ha H.R., Thomet U., Konrad D., Brecht K., Follath F., and Krahenbuhl S. Hepatocellular toxicity and pharmacological effect of amiodarone and amiodarone derivatives. Journal of Pharmacology and Experimental Therapeutics 319 3 (2006) 1413-1423
52. Wang K., Shindoh H., Inoue T., and Horii I. Advantages of in vitro cytotoxicity testing by using primary rat hepatocytes in comparison with established cell lines. Journal of Toxicological Sciences 27 3 (2002) 229-237
53. Wang Y.J., Liu H.L., Guo H.T., Wen H.W., and Liu J. Primary hepatocyte culture in collagen gel mixture and collagen sandwich. World Journal of Gastroenterology 10 5 (2004) 699-702
54. Wanson J.C., Drochmans P., Mosselmans R., and Ronveaux M.F. Adult rat hepatocytes in primary monolayer culture. Ultrastructural characteristics of intercellular contacts and cell membrane differentiations. The Journal of Cell Biology 74 3 (1977) 858-877
55. Wodnicka M., Guarino R.D., Hemperly J.J., Timmins M.R., Stitt D., and Pitner J.B. Novel fluorescent technology platform for high throughput cytotoxicity and proliferation assays. Journal of Biomolecular Screening 5 3 (2000) 141-152
56. Xing J.Z., Zhu L., Jackson J.A., Gabos S., Sun X.J., Wang X.B., and Xu X. Dynamic monitoring of cytotoxicity on microelectronic sensors. Chemical Research in Toxicology 18 2 (2005) 154-161
57. Zitova A., O'Mahony F.C., Cross M., Davenport J., and Papkovsky D.B. Toxicological profiling of chemical and environmental samples using panels of test organisms and optical oxygen respirometry. Environmental Toxicology 24 2 (2009) 116-127