The application of 3D micropatterning of agarose substrate for cell culture and in situ comet assays

Biomaterials

Volumn 31, Issue 12, 2010, Pages 3156-3165

  Mercey, Emilie ^a   Obeïd, Patricia ^a   Glaise, Denise ^b,c   Calvo Muñoz, Maria Luisa ^a   Guguen Guillouzo, Christiane ^b,c   Fouqué, Brigitte ^a  

^a CEA GRENOBLE   (France)

^b PONTCHAILLOU UNIVERSITY HOSPITAL   (France)

^c UNIVERSITÉ DE RENNES 1   (France)

Author keywords

3D micropatterning; Agarose; Cell array; Comet assay; Genotoxicity; HepaRG hepatocytes

Indexed keywords

3D MICROPATTERNING; AGAROSE; CELL ARRAY; COMET ASSAYS; GENOTOXICITIES; HEPATOCYTES;

ADHESION; ANIMAL CELL CULTURE; ASSAYS; CELL ADHESION; CELLS; DERIVATIVES; IMAGE ANALYSIS; THREE DIMENSIONAL;

CELL CULTURE;

AGAROSE; FIBRONECTIN;

ARTICLE; CELL ADHESION; CELL COUNT; CELL CULTURE; CELL DIFFERENTIATION; CELL FUNCTION; CELL POLARITY; CELL PROLIFERATION; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; GENOTOXICITY; HUMAN; HUMAN CELL; IMAGE ANALYSIS; LIVER TOXICITY; PRIORITY JOURNAL; TOXICITY TESTING; ULTRAVIOLET RADIATION;

CELL LINE, TUMOR; COMET ASSAY; EXTRACELLULAR MATRIX PROTEINS; FLUORESCENT DYES; HUMANS; MICROSCOPY, CONFOCAL; SEPHAROSE; ULTRAVIOLET RAYS;

EID: 76749114337     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2010.01.020     Document Type: Article

References (38)

1. Breuls R.G.M., Klumpers D.D., Everts V., and Smit T.H. Collagen type V modulates fibroblast behavior dependent on substrate stiffness. Biochem Biophys Res Commun 380 (2009) 425-429
2. Yeung T., Georges P.C., Flanagan L.A., Ortiz B.M.M., Funaki M., Zahir N., et al. Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion. Cell Motil Cytoskeleton 60 (2005) 24-34
3. Pelham R.J., and Yl W. Cell locomotion and focal adhesions are regulated by substrate. Proc Natl Acad Sci U S A 94 (1997) 13661-13665
4. Mann B.K., Gobin A.S., Tsai A.T., Schmedlen R.H., and West J.L. Smooth muscle cell growth in photopolymerized hydrogels with cell adhesive and proteolytically degradable domains: synthetic ECM analogs for tissue engineering. Biomaterials 22 (2001) 3045-3051
5. Tzvetkova-Chevolleau T., Stéphanou A., Fuard D., Ohayon J., Schiavone P., and Tracqui P. The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure. Biomaterials 29 (2008) 1541-1551
6. Nakanishi J., Takarada T., Yamaguchi K., and Maeda M. Recent advances in cell micropatterning techniques for bioanalytical and biomedical sciences. Anal Sci 24 (2008) 67-72
7. Hahn M.S., Taite L.J., Moon J.J., Rowland M.C., Ruffino K.A., and West J.L. Photolithographic patterning of polyethylene glycol hydrogels. Biomaterials 27 (2006) 2519-2524
8. Revzin A., Russell R.J., Yadavalli V.K., Koh W.G., Deister C., Hile D.D., et al. Fabrication of poly(ethylene glycol) hydrogel microstructures using photolithography. Langmuir 4 (2001) 5440-5447
9. Krsko P., McCann T.E., Thach T.T., Laabs T.L., Geller H.M., and Libera M.R. Length-scale mediated adhesion and directed growth of neural cells by surface-patterned poly(ethylene glycol) hydrogels. Biomaterials 30 (2009) 721-729
10. Lee S.H., Moon J.J., and West J.L. Three-dimensional micropatterning of bioactive hydrogels via two-photon laser scanning photolithography for guided 3D cell migration. Biomaterials 29 (2008) 2962-2968
11. Almany L., and Seliktar D. Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures. Biomaterials 26 (2005) 2467-2477
12. Lee K.Y., and Mooney D.J. Hydrogels fortissue engineering. Chem Rev 101 (2001) 1869-1880
13. Fedorovich N.E., Oudshoorn M.H., van Geemen D., Hennink W.E., Alblas J., and Dhert W.J.A. The effect of photopolymerization on stem cells embedded in hydrogels. Biomaterials 30 (2009) 344-353
14. Cheng S.Y., Heilman S., Wasserman M., Archer S., Shuler M., and Wu M. A hydrogel-based microfluidic device for the studies of directed cell migration. Lab Chip 7 (2007) 763-769
15. Flaim C.J., Chien S., and Bhatia S.N. An extracellular matrix microarray for probing cellular differentiation. Nat Meth 2 (2005) 119-125
16. Beningo K.A., Dembo M., and Yl W. Responses of fibroblasts to anchorage of dorsal extracellular matrix receptors. Proc Natl Acad Sci U S A 101 (2004) 18024-18029
17. Tourniaire G., Collins J., Campbell S., Mizomoto H., Ogawa S., Thaburet J., et al. Polymer microarrays for cellular adhesion. Chem Commun (Camb) 28 (2006) 2118-2120
18. Luo Y., and Shoichet M. A photolabile hydrogel for guided three-dimensional cell growth and migration. Nat Mater 3 (2004) 249-253
19. Nelson C.M., Raghavan S., Tan J.L., and Chen C.S. Degradation ofmicropatterned surfaces by cell-dependent and -independent processes. Langmuir 19 (2003) 1493-1499
20. Collins A.R., Oscoz A.A., Brunborg G., Gaivao I., Giovannelli L., Kruszewski M., et al. The comet assay: topical issues. Mutagenesis 23 (2008) 143-151
21. Tice R., and Strauss G. The single cell gel electrophoresis/comet assay: a potential tool for detecting radiation-induced DNA damage in humans. Stem Cells 13 (1995) 207-214
22. Hill H., and Hill G. Irradiation of cells attached or suspended by rubber policeman or by trypsin influences the extent of DNA strand breaks induced by ionizing radiation. Radiat Res 125 (1991) 343-345
23. Wischermann K., Boukamp P., and Schmezer P. Improved alkaline comet assay protocol for adherent HaCaT keratinocytes to study UVA-induced DNA damage. Mutat Res 630 (2007) 122-128
24. Aninat C., Piton A., Glaise D., Le Charpentier T., Langouet S., Morel F., et al. Expression of cytochromes P450, conjugating enzymes and nuclear receptors in human hepatoma HepaRG cells. Drug Metab Dispos 34 (2006) 75-83
25. Gripon P., Rumin S., Urban S., Le Seyec J., Glaise D., Cannie I., et al. Infection of a human hepatoma cell line by hepatitis B virus. Proc Natl Acad Sci U S A 99 (2002) 15655-15660
26. Cerec V., Glaise D., Garnier D., Morosan S., Turlin B., Drenou B., et al. Transdifferentiation of hepatocyte-like cells from the human hepatoma HepaRG cell line through bipotent progenitor. Hepatology 45 (2007) 957-967
27. Guillouzo A., Corlu A., Aninat C., Glaise D., Morel F., and Guguen-Guillouzo C. The human hepatoma HepaRG cells:a highly differentiated model for studies of liver metabolism and toxicity of xenobiotics. Chemico-Biological Interactions 168 (2007) 66-73
28. Afanassiev V., Hanemann V., and Wolfl S. Preparation of DNA and protein micro arrays on glass slides coated with an agarose film. Nucl Acids Res 28 (2000) e66
29. Azioune A., Storch M., Bornens M., Théry M., and Piel M. Simple and rapid process for single cell micro-patterning. Lab Chip 9 (2009) 1640-1642
30. Guguen-Guillouzo C. In: Freshney RI editor. Culture of animal cells: a manual of basic techniques, 6th edition. Wiley-Liss, in press.
31. Hazane F., Sauvaigo S., Douki T., Favier A., and Beani J.C. Age-dependent DNA repair and cell cycle distribution of human skin fibroblasts in response to UVA irradiation. J Photochem Photobiol B Biol 82 (2006) 214-223
32. Fink J., Théry M., Azioune A., Dupont R., Chatelain F., Bornens M., et al. Comparative study and improvement of current cell micro-patterning techniques. Lab Chip 7 (2007) 672-680
33. Takano H., Sul J.Y., Mazzanti M.L., Doyle R.T., Haydon P.G., and Porter M.D. Micropatterned substrates: approach to probing intercellular communication pathways. Anal Chem 74 (2002) 4640-4646
34. Sapsford K.E., and Ligler F.S. Real-time analysis of protein adsorption to a variety of thin films. Biosens Bioelectron 19 (2004) 1045-1055
35. Sakai S., Hashimoto I., and Kawakami K. Agarose-gelatin conjugate for adherent cell-enclosing capsules. Biotechnol Lett 29 (2007) 731-735
36. Lee L., Heimark R., Guzman R., Baygents J., and Zohar Y. Low melting point agarose as a protection layer in photolithographic patterning of aligned binary proteins. Lab Chip 6 (2006) 1080-1085
37. Collins A. The comet assay for DNA damage and repair. Mol Biotechnol 26 (2004) 249-261
38. Qureshi M., Bloom S.E., Hamilton J.W., and Dietert R.R. Toxic effects of methyl methanesulfonate (MMS) on activated macrophages from chickens. Environ Mol Mutagen 13 (1989) 253-262