Coupled bioconversion for preparation of N-acetyl-d-neuraminic acid using immobilized N-acetyl-d-glucosamine-2-epimerase and N-acetyl-d-neuraminic acid lyase

Applied Microbiology and Biotechnology

Volumn 85, Issue 5, 2010, Pages 1383-1391

  Hu, Shiyuan ^a,b   Chen, Jun ^a   Yang, Zhongyi ^c   Shao, Lijun ^a,b   Bai, Hua ^c   Luo, Jiali ^c   Jiang, Weihong ^a   Yang, Yunliu ^a  

^a Chinese Academy of Sciences   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c Zhejiang Hisun Pharmaceutical Co Ltd   (China)

Author keywords

Coupled reaction; Immobilized enzyme; N acetyl d glucosamine 2 epimerase; N acetyl d neuraminic acid; N acetyl d neuraminic lyase

Indexed keywords

CONVERSION RATES; COUPLED REACTION; ENZYMATIC PROCESS; EPIMERIZATION; IMMOBILIZED ENZYME; N-ACETYL-D-GLUCOSAMINE; N-ACETYL-D-GLUCOSAMINE 2-EPIMERASE; OXIRANE RESINS; PYRUVATES; REACTION CYCLES; REACTION TIME; SPECIFIC ACTIVITY; TWO-STEP REACTIONS;

ACIDS; AFFINITY CHROMATOGRAPHY; BIOCONVERSION; CATALYSTS; CHROMATOGRAPHIC ANALYSIS; ESCHERICHIA COLI; RESINS;

ENZYMES;

EPIMERASE; ETHYLENE OXIDE DERIVATIVE; LYASE; N ACETYL DEXTRO GLUCOSAMINE 2 EPIMERASE; N ACETYL DEXTRO NEURAMINIC ACID LYASE; N ACETYLGLUCOSAMINE; N ACETYLNEURAMINIC ACID; RESIN; UNCLASSIFIED DRUG;

AMINO ACID; CATALYSIS; COLIFORM BACTERIUM; ENZYME ACTIVITY; REACTION KINETICS; REACTION RATE; RESIN; TRANSFORMATION;

ARTICLE; BACTERIUM CULTURE; BIOREACTOR; BIOTRANSFORMATION; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION;

ACETYLGLUCOSAMINE; BIOREACTORS; CARBOHYDRATE EPIMERASES; CARRIER PROTEINS; CATALYSIS; CLONING, MOLECULAR; DNA, BACTERIAL; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; HEXOSAMINES; HYDROGEN-ION CONCENTRATION; KINETICS; N-ACETYLNEURAMINIC ACID; OXO-ACID-LYASES; PYRUVIC ACID; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 76649137965     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2163-9     Document Type: Article

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