Electrostatic focusing of unlabelled DNA into nanoscale pores using a salt gradient

Nature Nanotechnology

Volumn 5, Issue 2, 2010, Pages 160-165

  Wanunu, Meni ^a   Morrison, Will ^a   Rabin, Yitzhak ^b   Grosberg, Alexander Y ^c   Meller, Amit ^a  

^a Boston University   (United States)

^b BAR ILAN UNIVERSITY   (Israel)

^c POLYTECHNIC UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ELECTRIC FIELDS; SILICON NITRIDE; SOLID-STATE SENSORS;

ANALYTE CONCENTRATION; DNA CONCENTRATION; DNA TRANSLOCATION; ELECTRO-STATIC FOCUSING; HIGH THROUGHPUT; MOLECULAR DIMENSIONS; NANO-SCALE PORES; SOLID-STATE NANOPORE;

NANOPORES;

DOUBLE STRANDED DNA; NANOMATERIAL; SILICON NITRIDE; SODIUM CHLORIDE;

ARTICLE; DNA CONTENT; DNA DETERMINATION; DNA SEQUENCE; ELECTRIC FIELD; ELECTRICITY; HYDRODYNAMICS; IONIC STRENGTH; ISOELECTRIC FOCUSING; NANOPORE; PRIORITY JOURNAL;

EID: 76649116514     PISSN: 17483387     EISSN: 17483395     Source Type: Journal    
DOI: 10.1038/nnano.2009.379     Document Type: Article

References (27)

1. Li, J., Gershow, M., Stein, D., Brandin, E. & Golovchenko, J. A. DNA molecules and configurations in a solid-state nanopore microscope. Nature Mater. 2, 611-615 (2003).
2. Chen, P. et al. Probing single DNA molecule transport using fabricated nanopores. Nano Lett. 4, 2293-2298 (2004).
3. Storm, A. J. et al. Fast DNA translocation through a solid-state nanopore. Nano Lett. 5, 1193-1197 (2005).
4. Smeets, R. M. et al. Salt dependence of ion transport and DNA translocation through solid-state nanopores. Nano Lett. 6, 89-95 (2006).
5. Heng, J. B. et al. The electromechanics of DNA in a synthetic nanopore. Biophys. J. 90, 1098-1106 (2006).
6. McNally, B., Wanunu, M. & Meller, A. Electro-mechanical unzipping of individual DNA molecules using synthetic sub-2-nm pores. Nano Lett. 8, 3418-3422 (2008).
7. Wanunu, M., Sutin, J., McNally, B., Chow, A. & Meller, A. DNA translocation governed by interactions with solid state nanopores. Biophys. J. 95, 4716-4725 (2008).
8. Smeets, R. M. M., Kowalczyk, S. W., Hall, A. R., Dekker, N. H. & Dekker, C. Translocation of recA-coated double-stranded DNA through solid-state nanopores. Nano Lett. 9, 3089-3095 (2009).
9. Wanunu, M., Sutin, J. & Meller, A. DNA profiling using solid-state nanopores: detection of DNA-binding molecules. Nano Lett. 9, 3498-3502 (2009).
10. Branton, D. et al. The potential and challenges of nanopore sequencing. Nature Biotechnol. 26, 1146-1153 (2008).
11. Clarke, J. et al. Continuous base identification for single-molecule nanopore DNA sequencing. Nature Nanotech. 4, 265-270 (2009).
12. Henrickson, S. E., Misakian, M., Robertson, B. & Kasianowicz, J. J. Driven DNA transport into an asymmetric nanometer scale pore. Phys. Rev. Lett. 85, 3057-3060 (2000).
13. Meller, A. & Branton, D. Single molecule measurements of DNA transport through a nanopore. Electrophoresis 23, 2583-2591 (2002).
14. Meller, A. Dynamics of polynucleotide transport through nanometre-scale pores. J. Phys. Condens. Matter 15, R581-R607 (2003).
15. Zhang, J. & Shklovskii, B. I. Effective charge and free energy of DNA inside an ion channel. Phys. Rev. E 75, 021906 (2007).
16. Gershow, M. & Golovchenko, J. A. Recapturing and trapping single molecules with a solid-state nanopore. Nature Nanotech. 2, 775-779 (2007).
17. Kim, M. J., Wanunu, M., Bell, D. C. & Meller, A. Rapid fabrication of uniformly sized nanopores and nanopore arrays for parallel DNA analysis. Adv. Mater. 18, 3149-3153 (2006).
18. Schmidt, C., Mayer, M. & Vogel, H. A chip-based biosensor for the functional analysis of single ion channels. Angew Chem. Int. Ed. 39, 3137-3140 (2000).
19. Long, D., Viovy, J.-L. & Ajdari, A. Simultaneous action of electric fields and nonelectric forces on a polyelectrolyte: motion and deformation. Phys. Rev. Lett. 76, 3858-3861 (1996).
20. Nkodo, A. E. et al. Diffusion coefficient of DNA molecules during free solution electrophoresis. Electrophoresis 22, 2424-2432 (2001).
21. Olivera, B. M., Baine, P. & Davidson, N. Electrophoresis of the nucleic acids. Biopolymers 2, 245-257 (1964).
22. Wong, C. T. A. & Muthukumar, M. Polymer capture by electro-osmotic flow of oppositely charged nanopores. J. Chem. Phys. 126, 164903 (2007).
23. Chou, T. Enhancement of charged macromolecule capture by nanopores in a salt gradient. J. Chem. Phys. 131, 034703 (2009).
24. Luan, B. & Aksimentiev, A. Electro-osmotic screening of the DNA charge in a nanopore. Phys. Rev. E 78, 021912 (2008).
25. Dorp, S. V., Keyser, U. F., Dekker, N. H., Dekker, C. & Lemay, S. G. Origin of the electrophoretic force on DNA in solid-state nanopores. Nature Phys. 5, 347-351 (2009).
26. Wanunu, M. & Meller, A. Chemically-modified solid-state nanopores. Nano Lett. 7, 1580-1585 (2007).
27. Wanunu, M. & Meller, A. Single Molecule Analysis of Nucleic Acids and DNA-Protein Interactions using Nanopores (Cold Spring Harbor Press, 2008).