Isolation of functional single cells from environments using a micromanipulator: Application to study denitrifying bacteria

Applied Microbiology and Biotechnology

Volumn 85, Issue 4, 2010, Pages 1211-1217

  Ashida, Naoaki ^a   Ishii, Satoshi ^a   Hayano, Sadakazu ^a   Tago, Kanako ^a   Tsuji, Takashi ^b   Yoshimura, Yoshitaka ^b   Otsuka, Shigeto ^a   Senoo, Keishi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b TAMAGAWA UNIVERSITY   (Japan)

Author keywords

Denitrification; Direct viable counts; Live cell staining; Micromanipulation; Rice paddy soil; Single cell isolation

Indexed keywords

CELL ISOLATION; DIRECT VIABLE COUNTS; MICRO MANIPULATION; RICE PADDY; SINGLE-CELL ISOLATION;

BACTERIA; BACTERIOLOGY; DENITRIFICATION; ESTERS; MICROMANIPULATORS; RNA; SOILS; SOLAR CONCENTRATORS;

CELLS;

6 CARBOXYFLUORESCEIN; 6 CARBOXYFLUORESCEIN DIACETATE ACETOXYMETHYL ESTER; COPPER; NITRITE REDUCTASE; RNA 16S; UNCLASSIFIED DRUG;

AGRICULTURAL SOIL; BACTERIUM; BIOCHEMISTRY; DENITRIFICATION; ESTER; GENE EXPRESSION; PADDY FIELD; SUBSTRATE;

ARTICLE; AZOSPIRILLUM; BACTERIAL CELL; BACTERIAL GENE; BACTERIUM ISOLATION; CONTROLLED STUDY; DENITRIFYING BACTERIUM; GAS CHROMATOGRAPHY; ION PAIR CHROMATOGRAPHY; MICROMANIPULATION; MICROMANIPULATOR; NONHUMAN; NUCLEOTIDE SEQUENCE; OCHROBACTRUM; RICE; SOIL MICROFLORA; SPECIES CULTIVATION; STAINING;

AZOSPIRILLUM; BACTERIA; BACTERIOLOGICAL TECHNIQUES; DNA, BACTERIAL; DNA, RIBOSOMAL; FLUORESCEINS; NITRATES; OCHROBACTRUM; ORYZA SATIVA; PHYLOGENY; RNA, RIBOSOMAL, 16S; SOIL MICROBIOLOGY;

AZOSPIRILLUM; OCHROBACTRUM;

EID: 76649114651     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2330-z     Document Type: Article

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