Quantitative analysis of the cutaneous Malassezia microbiota in 770 healthy Japanese by age and gender using a real-time PCR assay

Medical Mycology

Volumn 48, Issue 2, 2010, Pages 229-233

  Sugita, Takashi ^a   Suzuki, Miho ^a   Goto, Seiko ^a   Nishikawa, Akemi ^a   Hiruma, Masataro ^b   Yamazaki, Takashi ^c   Makimura, Koichi ^d  

^a MEIJI PHARMACEUTICAL UNIVERSITY   (Japan)

^b JUNTENDO UNIVERSITY NERIMA HOSPITAL   (Japan)

^c JAPAN AEROSPACE EXPLORATION AGENCY   (Japan)

^d TEIKYO UNIVERSITY   (Japan)

Author keywords

Age; Healthy subject; Malassezia microbiota; Quantitative analysis; Real time polymerase chain reaction

Indexed keywords

ACCURACY; ADOLESCENT; ADULT; AGED; ARTICLE; CELL COUNT; CHILD; CONTROLLED STUDY; DNA PROBE; FEMALE; FUNGAL CELL; FUNGAL COLONIZATION; FUNGUS; HUMAN; HUMAN EXPERIMENT; INFANT; JAPANESE; MALASSEZIA; MALASSEZIA GLOBOSA; MALASSEZIA RESTRICTA; MALE; MICROFLORA; NEWBORN; NORMAL HUMAN; PRESCHOOL CHILD; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SCHOOL CHILD; SENESCENCE; SEX DIFFERENCE; SKIN FLORA; AGE; CHEEK; CHEMISTRY; EPIDEMIOLOGY; GENETICS; ISOLATION AND PURIFICATION; JAPAN; METAGENOME; MICROBIOLOGY; MIDDLE AGED; POLYMERASE CHAIN REACTION; PROCEDURES; SKIN; SPECIES DIFFERENCE; VERY ELDERLY;

ADOLESCENT; ADULT; AGE FACTORS; AGED; AGED, 80 AND OVER; CHEEK; CHILD; CHILD, PRESCHOOL; DNA, FUNGAL; FEMALE; HUMANS; INFANT; JAPAN; MALASSEZIA; MALE; METAGENOME; MIDDLE AGED; POLYMERASE CHAIN REACTION; SEX FACTORS; SKIN; SPECIES SPECIFICITY;

MALASSEZIA; MALASSEZIA GLOBOSA; MALASSEZIA RESTRICTA;

FUNGAL DNA;

EID: 76649107370     PISSN: 13693786     EISSN: 14602709     Source Type: Journal    
DOI: 10.3109/13693780902977976     Document Type: Article

References (20)

1. Guého E, Midgley G, Guillot J. The genus Malassezia with description of four new species. Antonie Van Leeuwenhoek 1996; 69: 337-355.
2. Sugita T, Takashima M, Shinoda T, e t al. New yeast species, M alassezia dermatis, isolated from patients with atopic dermatitis. J Clin Microbiol. 2002; 40: 1363-1367.
3. Sugita T, Takashima M, Kodama M, e t al. Description of a new yeast species, Malassezia japonica, and its detection in patients with atopic dermatitis and healthy subjects. J Clin Microbiol 2003; 41: 4695-4699.
4. Sugita T, Tajima M, Takashima M, e t al. A new yeast, M alassezia yamatoensis, isolated from a patient with seborrheic dermatitis, and its distribution in patients and healthy subjects. Microbiol Immunol. 2004; 48: 579-583.
5. Hirai A, Kano R, Makimura K, e t al. M alassezia nana sp. nov., a novel lipid-dependent yeast species isolated from animals. Int J Syst Evol Microbiol 2004; 54: 623-627.
6. Cabañes FJ, Theelen B, Castellá G, Boekhout T. Two new lipid-dependent Malassezia species from domestic animals. FEMS Yeast Res. 2007; 7: 1064-1076.
7. Gupta AK, Batra R, Bluhm R, et al. Skin diseases associated with Malassezia species. J Am Acad Dermatol 2004; 51: 785-798.
8. Batra R, Boekhout T, Guého E, et al. Malassezia Baillon, emerging clinical yeasts. FEMS Yeast Res 2005; 5: 1101-1113.
9. Ashbee HR. Update on the genus Malassezia. Med Mycol 2007; 45: 287-303.
10. Takahata Y, Sugita T, Hiruma M, Muto M. Quantitative analysis of Malassezia in the scale of patients with psoriasis using a real-time polymerase chain reaction assay. Br J Dermatol 2007; 157: 670-673.
11. Amaya M, Tajima M, Okubo Y, et al. Molecular analysis of M alassezia microflora in the lesional skin of psoriasis patients. J Dermatol 2007; 34: 619-624.
12. Nakabayashi A, Sei Y, Guillot J. Identification of Malassezia species isolated from patients with seborrhoeic dermatitis, atopic dermatitis, pityriasis versicolor and normal subjects. Med Mycol 2000; 38: 337-341.
13. Gupta AK, Kohli Y, Summerbell RC, Faergemann J. Quantitative culture of Malassezia species from different body sites of individuals with or without dermatoses. Med Mycol 2001; 39: 243-251.
14. Sugita T, Suto H, Unno T, et al. Molecular analysis of M alassezia microflora on the skin of atopic dermatitis patients and healthy subjects. J Clin Microbiol 2001; 39: 3486-3490.
15. Sugita T, Tajima M, Tsubuku H, et al. Quantitative analysis of cutaneous Malassezia in atopic dermatitis patients using real-time PCR. Microbiol Immunol 2006; 50: 549-552.
16. Gupta AK, Kohli Y. Prevalence of Malassezia species on various body sites in clinically healthy subjects representing different age groups. Med Mycol 2004; 42: 35-42.
17. Pochi PE, Strauss JS, Downing DT. Age-related changes in sebaceous gland activity. J Invest Dermatol 1979; 73: 108-111.
18. Tajima M, Sugita T, Nishikawa A, Tsuboi R. Molecular analysis of Malassezia microflora in seborrheic dermatitis patients: comparison with other diseases and healthy subjects. J Invest Dermatol 2008; 128: 345-351.
19. Sugita T. Mycological study on M alassezia. Nippon Ishinkin Gakkai Zasshi 2007; 48: 179-182.
20. Yamamoto A, Serizawa S, Ito M, Sato Y. Effect of aging on sebaceous gland activity and on the fatty acid composition of wax esters. J Invest Dermatol 1987; 89: 507-512