Rapid detection and differentiation of Erysipelothrix spp. by a novel multiplex real-time PCR assay

Journal of Applied Microbiology

Volumn 108, Issue 3, 2010, Pages 1083-1093

  Pal, N ^a   Bender, J S ^a   Opriessnig, T ^a  

^a IOWA STATE UNIVERSITY   (United States)

Author keywords

Erysipelothrix spp; Multiplex PCR; Pigs

Indexed keywords

MAMMALS; POLYMERASE CHAIN REACTION; RNA;

DIRECT-DETECTION; ENRICHMENT ISOLATIONS; ERYSIPELOTHRIX SPP; MULTIPLEX PCR; PIG; PRIMER SETS; RAPID DETECTION; RAPID DIFFERENTIATION; REAL-TIME PCR ASSAY; SPECIES SPECIFICS;

PROBES;

BACTERIAL DNA; RNA 5S;

BACTERIUM; BIOASSAY; CULTIVATION; PIG; POLYMERASE CHAIN REACTION; REAL TIME; RNA;

ANIMAL EXPERIMENT; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; COLONY FORMING UNIT; CROSS REACTION; DNA EXTRACTION; ERYSIPELOTHRIX; ERYSIPELOTHRIX RHUSIOPATHIAE; ERYSIPELOTHRIX TONSILLARUM; GENOME; INOCULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEPTICEMIA; SEROTYPE; SPECIES DIFFERENCE; SUBSPECIES; SWINE;

ANIMALIA; BACTERIA (MICROORGANISMS); ERYSIPELOTHRIX; ERYSIPELOTHRIX RHUSIOPATHIAE; ERYSIPELOTHRIX SP.; SUIDAE;

EID: 76449114780     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2009.04560.x     Document Type: Article

References (29)

1. Ahrne, S., Stenstrom, I., Jensen, N.E., Pettersson, B., Uhlen, M. Molin, G. (1995) Classification of Erysipelothrix strains on the basis of restriction fragment length polymorphisms. Int J Syst Bacteriol 45, 382 385.
2. Bender, J.S., Kinyon, J.M., Kariyawasam, S., Halbur, P.G. Opriessnig, T. (2009) Comparison of conventional direct and enrichment culture methods for Erysipelothrix spp. from experimentally and naturally infected swine. J Vet Diagn Invest 21, 863 868.
3. Brooke, C.J. Riley, T.V. (1999) Erysipelothrix rhusiopathiae: bacteriology, epidemiology and clinical manifestations of an occupational pathogen. J Med Microbiol 48, 789 799.
4. Chooromoney, K.N., Hampson, D.J., Eamens, G.J. Turner, M.J. (1994) Analysis of E. rhusiopathiae and E. tonsillarum by multilocus enzyme electrophoresis. J Clin Microbiol 32, 371 376.
5. Hassanein, R., Sawada, T., Kataoka, Y., Ali, M., Itoh, K. Suzuki, Y. (2000) Prevalence of Erysipelothrix species in healthy, slaughtered cattle. J Vet Epidemiol 4, 71 79.
6. Hassanein, R., Sawada, T., Kataoka, Y., Itoh, K. Suzuki, Y. (2001) Serovars of Erysipelothrix species isolated from the tonsils of healthy cattle in Japan. Vet Microbiol 82, 97 100.
7. Hassanein, R., Sawada, T., Kataoka, Y., Gadallah, A. Suzuki, Y. (2003) Molecular identification of Erysipelothrix isolates from the tonsils of healthy cattle by PCR. Vet Microbiol 95, 239 245. (Pubitemid 37297602)
8. Jones, D. (1986) Genus Erysipelothrix. In Berger's Manual of Systematic Bacteriology ed. Sneath, P.H.A., Mair, N.S., Sharpe, M.E. Holt, J.G., Vol. 2, pp. 1245 1249. London : Williams & Wilkins.
9. Kucsera, G. (1979) Serological typing of E. rhusiopathiae strains and the epidemiological significance of the typing. Acta Acad Sci Hung 27, 19 28.
10. Landis, J. Koch, G. (1977) The measurement of observer agreement for categorical data. Biometrics 33, 159 174.
11. Makino, S., Okada, Y., Maruyama, T., Ishikawa, K., Takahashi, T., Nakumura, M., Ezaki, T. Morita, H. (1994) Direct and rapid detection of Erysipelothrix rhusiopathiae DNA in animals by PCR. J Clin Microbiol 32, 1526 1531.
12. Norrung, V. Molin, G. (1991) A new serotype of Erysipelothrux rhusiopathiae isolated from pig slurry. Acta Vet Hung 39, 137 138.
13. Okatani, A.T., Hayashidani, H., Takahashi, T., Taniguchi, T., Ogawa, M. Kaneko, K. (2000) Randomly amplified DNA analysis of Erysipelothrix spp. J Clin Microbiol 38, 4332 4336.
14. Opriessnig, T., Hoffman, L.J., Harris, D.L., Gaul, S.B. Halbur, P.G. (2004) Erysipelothrix rhusiopathiae: genetic characterization of Midwest US isolates and live commercial vaccines using pulse-field gel electrophoresis. J Vet Diagn Invest 16, 101 107.
15. Prickett, J., Simer, R., Christopher Hennings, J., Yoon, K.J., Evans, R.B. Zimmerman, J.J. (2008) Detection of Porcine Reproductive and Respiratory Syndrome virus infection in porcine oral fluid samples: a longitudinal study under experimental conditions. J Vet Diagn Invest 20, 156 163.
16. Shimoji, Y., Mori, Y., Hyakutake, K., Sekizaki, T. Yokomizo, Y. (1998) Use of an enrichment broth cultivation-PCR combination assay for rapid diagnosis of swine erysipelas. J Clin Microbiol 36, 86 89.
17. Takahashi, T., Fujisawa, T., Benno, Y., Tamura, Y., Sawada, T., Suzuki, S., Muramatsu, M. Mitsuoka, T. (1987a) Erysipelothrix tonsillarum sp. nov. isolated from tonsils of apparently healthy pigs. Int J Syst Bacteriol 37, 166 168.
18. Takahashi, T., Sawada, T., Muramatsu, M., Tamura, Y., Fujisawa, T., Benno, Y. Mitsuoka, T. (1987b) Serotype, antimicrobial susceptibility, and pathogenicity of Erysipelothrix rhusiopathiae isolates from tonsils of apparently healthy slaughter pigs. J Clin Microbiol 25, 536 553.
19. Takahashi, T., Fujisawa, T., Tamura, Y., Suzuki, S., Muramatsu, M., Sawada, T., Benno, Y. Mitsuoka, T. (1992) DNA relatedness among Erysipelothrix rhusiopathiae strains representing all twenty-three serovars and Erysipelothrix tonsillarum. Int J Syst Bacteriol 42, 469 473.
20. Takahashi, T., Nagamine, N., Kijima, M., Suzuki, S., Takagi, M., Tamura, Y., Nakamura, M., Muramatsu, M. et al. (1996) Serovars of Erysipelothrix strains isolated from pigs affected with erysipelas in Japan. J Vet Med Sci 58, 587 589.
21. Takahashi, T., Fujisawa, T., Umeno, A., Kozasa, T., Yamamoto, K. Sawada, T. (2008) A taxonomic study on erysipelothrix by DNA-DNA hybridization experiments with numerous strains isolated from extensive origins. Microbiol Immunol 52, 469 478.
22. Takeshi, K., Makino, S., Ikeda, T., Takada, N., Nakashiro, A., Nakanishi, K., Oguma, K., Katoh, Y. et al. (1999) Direct and rapid detection by PCR of Erysipelothrix sp. DNAs prepared from bacterial strains and animal tissues. J Clin Microbiol 37, 4093 4098.
23. Wood, R.L. (1965) A selective liquid medium utilizing antibiotics for isolation of Erysipelothrix insidiosa. Am J Vet Res 26, 1303 1308.
24. Wood, R.L. (1984) Swine erysipelas - a review of prevalence and research. J Am Vet Med Assoc 184, 944 949.
25. Wood, R.L. (1999) Erysipelas. In Diseases of Swine ed. Straw, B.E., D'Allaire, S., Mengeling, W.L. Taylor, D.J., 8th edn, pp. 419 430. Ames : Iowa State University Press.
26. Wood, R.L. Harrington, R. (1978) Serotypes of Erysipelothrix rhusiopathiae isolated from swine and soil and manure of swine pens in the United States. Am J Vet Res 39, 1833 1840.
27. Xu, K., Hu, X., Gao, C. Lu, Q. (1984) A new serotype of Erysipelothrix rhusiopathiae. Anim Infect Dis 4, 11 14.
28. Xu, K., Gao, C. Hu, X. (1986) Study on a new serotype of Erysipelothrix rhusiopathiae isolated from marine fishes. Anim Infect Dis 3, 6 7.
29. Yamazaki, Y. (2006) A multiplex polymerase chain reaction for discriminating Erysipelothrix rhusiopathiae from Erysipelothrix tonsillarum. J Vet Diagn Investig 18, 384 387.