The role of hydrophobic interactions in catalysis of RNA cleavage by 1,4-diazabicyclo[2.2.2]-octane based artificial ribonucleases

Nucleosides, Nucleotides and Nucleic Acids

Volumn 23, Issue 6-7, 2004, Pages 907-913

  Kuznetsova, I ^a   Tuzikov, F ^b   Tuzikova, N ^b   Tamkovich, N ^a   Zenkova, M ^a   Vlassov, V ^a  

^a INSTITUTE OF CHEMICAL BIOLOGY AND FUNDAMENTAL MEDICINE   (Russian Federation)

^b STATE RESEARCH CENTER OF VIROLOGY AND BIOTECHNOLOGY VECTOR   (Russian Federation)

Author keywords

Artificial ribonuclease; Light scattering; Micelle formation; RNA cleavage; Small angle x ray scattering

Indexed keywords

1,4 DIAZABICYCLO[2.2.2]OCTANE; IMIDAZOLE; RIBONUCLEASE;

CATALYSIS; CONFERENCE PAPER; CONJUGATE; ELECTROPHORESIS; HYDROPHOBICITY; LIGHT SCATTERING; MICELLIZATION; MOLECULAR INTERACTION; PARTICLE SIZE; RNA CLEAVAGE; RNA STRUCTURE; X RAY CRYSTALLOGRAPHY;

CATALYSIS; HYDROLYSIS; MICELLES; PIPERAZINES; RNA; SCATTERING, RADIATION;

EID: 7644226186     PISSN: 15257770     EISSN: None     Source Type: Journal    
DOI: 10.1081/NCN-200026039     Document Type: Conference Paper

References (10)

1. Konevets, D.A.; Bekk, I.E.; Sil'nikov, V.N.; Zenkova, M.A.; Shishkin, G.V. Chemical ribonucleases. 3. Synthesis of organic catalysts of hydrolysis of phosphodiester bonds based on quaternary salts of 1,4-diazabicyclo(2.2.2)octane. Bioorg. Khim 2000, 26 (11), 852-861. (Russian).
2. Zenkova, M.; Beloglazova, N.; Sil'nikov, V.; Vlassov, V.; Giege, R. RNA cleavage by 1,4-diazabicyclo[2.2.2]octane-imidazole conjugates. Methods Enzymol. 2001, 347, 468-490.
3. Svergun, D.I.; Feigin, L.A. X-Ray and Neutron Small-Angle Scattering (Russian); Nauka: Moscow, 1986.
4. Phe. FEBS Lett. 1988, 232, 107-110.
5. Gill, P.E.; Murray, W.; Wright, M.H. Practical Optimization; Academic Press: London, 1981.
6. Tuzikov, F.V.; Tuzikova, N.A.; Naumochkin, A.N.; Zinoviev, V.V.; Malygin, E.G. Fluorescence quenching study of equilibrium binding of phage T4 dam DNA-[N-adenine]-methyltransferase with substrates and ligands. Russ. J. Mol. Biol. 1997, 37, 73-76.
7. Tyulkina, L.G.; Mankin, A.S. Inhibition of ribonuclease contaminations in preparation of T4 RNA ligase, polynucleotide kinase and bacterial alkaline phosphatase with bentonite. Anal. Biochem. 1984, 138, 285-290.
8. Giege, R.; Felden, B.; Zenkova, M.A.; Sil'nikov, V.N.; Vlassov, V.V. Cleavage of RNA with synthetic ribonuclease mimics. Methods Enzymol. 2000, 318, 147-165.
9. Podyminogin, M.A.; Vlassov, V.V.; Giege, R. Synthetic RNA-cleaving molecules mimicking ribonuclease A active center. Design and cleavage of tRNA transcripts. Nucleic Acids Res. 1993, 21, 5950-5956.
10. Tuzikov, F.V.; Zinoviev, V.V.; Vavilin, V.I.; Malygin, E.G. Application of the small-angle x-ray scattering technique for the study of two-step equilibrium enzyme-substrate interactions. Biopolymers 1996, 38, 131-139.