Quantitative determination of spatial protein-protein correlations in fluorescence confocal microscopy

Biophysical Journal

Volumn 98, Issue 3, 2010, Pages 493-504

  Wu, Yong ^a   Eghbali, Mansoureh ^a   Ou, Jimmy ^a   Lu, Rong ^a   Toro, Ligia ^a,b   Stefani, Enrico ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 76249109684     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2009.10.037     Document Type: Article

References (22)

1. Fox, M. H., D. J. Arndt-Jovin, ..., M. Robert-Nicoud. 1991. Spatial and temporal distribution of DNA replication sites localized by immunofluorescence and confocal microscopy in mouse fibroblasts. J. Cell Sci. 99:247-253.
2. 2. Dutartre, H., J. Davoust, ..., P. Chavrier. 1996. Cytokinesis arrest and redistribution of acrin-cytoskeleton regulatory components in cells expressing the pGTPase CDC42Hs. J. Cell Sci. 109:367-377. (Pubitemid 26057119)
3. Manders, E. M., J. Stap, ..., J. A. Aten. 1992. Dynamics of three-dimensional replication patterns during the S-phase, analyzed by double labeling of DNA and confocal microscopy. J. Cell Sci. 103:857-862.
4. 4. Demandolx, D., and J. Davoust. 1997. Multicolor analysis and local image correlation in confocal microscopy. J. Microsc 185:21-36. (Pubitemid 27060523)
5. Manders, E. M., F. J. Verbeek, and J. A. Aten. 1993. Measurement of colocalization of objects in dual-color confocal images. J. Microsc. 169:375-382.
6. 6. Li, Q., A. Lau, ..., E. F. Stanley. 2004. A syntaxin 1, Gα(o), and N-type calcium channel complex at a presynaptic nerve terminal: analysis by quantitative immunocolocalization. J. Neurosci. 24:4070-4081. (Pubitemid 38544233)
7. Costes, S. V., D. Daelemans, ..., S. Lockert. 2004. Automatic and quantitative measurement of protein-protein, colocalization in live cells. Biophys. J. 86:3993-4003.
8. Wiseman, P. W., J. A. Squier, ..., K. R. Wilson. 2000. Two-photon image correlation spectroscopy and image cross-correlation spectroscopy. J. Microsc. 200:14-25.
9. 9. Costantino, S., J. W. Comeau, ..., P. W. Wiseman. 2005. Accuracy and dynamic range of spatial image correlation and cross-correlation spectroscopy. Biophys. J. 89:1251-1260. (Pubitemid 41099006)
10. Comeau, J. W., S. Costantino, and P. W. Wiseman. 2006. A guide to accurate fluorescence microscopy colocalization measurements. Biophys, J. 91:4611-4622.
11. Comeau, J. W., D. L. Kolin, and P. W. Wiseman. 2008. Accurate measurements of protein interactions in cells via improved spatial image cross-correlation spectroscopy. Mol. Biosyst. 4:672-685.
12. Wiseman, P. W., and N. O. Petersen. 1999. Image correlation spectroscopy. II. Optimization for ultrasensitive detection of preexisting platelet-derived growth factor-β receptor oligomers on intact cells. Biophys. J. 76:963-977.
13. Reference deleted in proof.
14. 14. Scriven, D. R. L., P. Dan, and E. D. W. Moore. 2000. Distribution of proteins implicated in excitation-contraction coupling in rat ventricular myocytes. Biophys. J. 79:2682-2691. (Pubitemid 30847865)
15. Ropero, A. B., M. Eghbali, ..., E. Stefani. 2006. Heart estrogen receptor alpha: distinct membrane and nuclear distribution patterns and regulation by estrogen, J. Mol. Cell. Cardiol. 41:496-510.
16. 2+- and thromboxane-dependent distribution of MaxiK channels in cultured astrocytes: from microtubules to the plasma membrane. Glia. 57:1280-1295.
17. Lu, R., A. Alloua, ..., L. Toro. 2008. c-Src tyrosine kinase, a critical component for 5-HT2A receptor-mediated contraction in rat aorta. J. Physiol. 586:3855-3869.
18. 18. Bolte, S., and F. P. Cordelières. 2006. A guided tour into subcellular colocalization analysis in light microscopy. J. Microsc. 224:213-232. (Pubitemid 46010930)
19. Petersen, N. O., P. L. Höddelius, ..., K. E. Magnusson. 1993. Quantitation of membrane receptor distributions by image correlation spectroscopy: concept and application. Biophys. J. 65:1135-1146.
20. Nohe, A., and N. O. Petersen. 2007. Image correlation spectroscopy. Sci. STKE. 2007:p17.
21. 21. Kenworthy, A. K. 2001. Imaging protein-protein interactions using fluorescence resonance energy transfer microscopy. Methods. 24:289-296. (Pubitemid 32846434)
22. 22. König, P., G. Krasteva, ..., W. Rummer. 2006. FRET-CLSM and double-labeling indirect immunofluorescence to detect close association of proteins in tissue sections. Lab. Invest. 86:853-864. (Pubitemid 44100483)