Molecular characterization of serogrouping and virulence genes of Malaysian Vibrio cholerae isolated from different sources

Journal of General and Applied Microbiology

Volumn 55, Issue 6, 2009, Pages 419-425

  Teh, Cindy Shuan Ju ^a   Thong, Kwai Lin ^a   Ngoi, Soo Tein ^a   Ahmad, Norazah ^b   Nair, Gopinath Balakrish ^c   Ramamurthy, Thandavarayan ^c  

^a UNIVERSITY OF MALAYA   (Malaysia)

^b INSTITUTE FOR MEDICAL RESEARCH   (Malaysia)

^c NATIONAL INSTITUTE OF CHOLERA AND ENTERIC DISEASES   (India)

Author keywords

Multiplex PCR; Serogroup; Vibrio cholerae; Virulence gene

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM DETECTION; BACTERIUM ISOLATION; BLOOD GROUP TYPING; CLINICAL EXAMINATION; CONTROLLED STUDY; CTXA GENE; FECES ANALYSIS; GENE ISOLATION; GENE TARGETING; GENETIC TRAIT; HLYA GENE; INTERMETHOD COMPARISON; MALAYSIA; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; OMPW GENE; RELIABILITY; REPRODUCIBILITY; SEA FOOD; STRAIN IDENTIFICATION; TCPI GENE; TOXR GENE; VIBRIO CHOLERAE; WATER SAMPLING;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; CHOLERA; DNA PRIMERS; HEMOLYSIN PROTEINS; HUMANS; MALAYSIA; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEAFOOD; SEAWATER; SENSITIVITY AND SPECIFICITY; SEROTYPING; VIBRIO CHOLERAE; VIRULENCE;

VIBRIO CHOLERAE;

EID: 76149112916     PISSN: 00221260     EISSN: 13498037     Source Type: Journal    
DOI: 10.2323/jgam.55.419     Document Type: Article

References (22)

1. Alam, M., Sadique, A., Nur-A-Hasan, Bhuiyan, N. A., Nair, G. B., Siddique, A. K., Sack, D. A., Ahsan, S., Huq, A., Sack, R. B., and Colwell, R. R. (2006) Effect of transport at ambient temperature on detection and isolation of Vibrio cholerae from environmental samples. Appl. Environ. Microbiol., 72, 2185-2190.
2. Chakraborty, S., Mukhopadhyay, A. K., Bhadra, R. K., Ghosh, A. N., Mitra, R., Shimada, T., Yamasaki, S., Faruque, S. M., Takeda, Y., Colwell, R. R., and Nair, G. B. (2000) Virulence genes in environmental strains of Vibrio cholerae. Appl. Environ. Microbiol., 66, 4022-4028.
3. + strains of Vibrio cholerae isolated from seafood in Malaysia. Appl. Environ. Microbiol., 70, 1964-1972.
4. Dalsgaard, A., Serichantalergs, O., Forslund, A., Lin, W., Mekalanos, J., Mintz, E., Shimada, T., and Wells, J. G. (2001) Clinical and environmental isolates of Vibrio cholerae serogroup O141 carry CTX phage and genes encoding the toxin-coregulated pili. J. Clin. Microbiol., 39, 4086-4092.
5. Danin-Poleg, Y., Cohen, L. A., Gancz, H., Broza, Y. Y., Goldshmidt, H., Mallul, E., Valinsky, L., Lerner, L., Broza, M., and Kashi, Y. (2007) Vibrio cholerae strain typing and phylogeny study based on simple sequence repeats. J. Clin. Microbiol., 45, 736-746.
6. Faruque, S. M., Albert, M. J., and Mekalanos, J. J. (1998) Epidemiology, genetics and ecology of toxigenic Vibrio cholera. Microbiol. Mol. Biol. Rev., 62, 1301-1314.
7. Faruque, S. M., Chowdhury, N., Kamruzzaman, M., Dziejman, M., Rahman, M. H., Nair, G. B., and Mekalanos, J. J. (2004) Genetic diversity and virulence potential of environmental Vibrio cholerae population in a cholera-endemic area. Proc. Natl. Acad. Sci. USA, 101, 2123-2128.
8. Fields, P. I., Popovic, T., Wachsmuth, K., and Olsvik, O. (1992) Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemics. J. Clin. Microbiol., 30, 2128-2121.
9. Hoshino, K., Yamasaki, S., Mukhopadhyay, A. K., Chakraborty, S., Basu, A., Bhattacharya, S. K., Nair, G. B., Shimada, T., and Takeda, Y. (1998) Development and evaluation of multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139. FEMS Immunol. Med. Microbiol., 20, 201-207.
10. Keymer, D. P., Miller, M. C., Schoolnik, G. K., and Boehm, A. (2007) Genomic and phenotypic diversity of coastal Vibrio cholerae strains is linked to environmental factors. Appl. Environ. Microbiol., 73, 3705-3714.
11. Mantri, C. K., Mohapatra, S. S., Ramamurthy, T., Ghosh, R., Colwell, R. R., and Singh, D. V. (2006) Septaplex PCR assay for rapid identification of Vibrio cholerae including detection of virulence and int SXT genes. FEMS Microbiol. Lett., 265, 208-214.
12. Nandi, B., Nandy, R. K., Mukhopadhyay, S., Nair, G. B., Shimada, T., and Ghose, A. C. (2000) Rapid method for species-specific identification of Vibrio cholerae using primers targeted to the gene of outer membrane protein OmpW. J. Clin. Microbiol., 38, 4145-4151.
13. Ottaviani, D., Masini, L., and Bachiocchi, S. (2003) A biochemical protocol for the isolation and identification of current species of Vibrio in seafood. J. Appl. Microbiol., 95, 1277-1284.
14. Peterson, K. M. (2002) Expression of Vibrio cholerae virulence genes in response to environmental signals. Curr. Issues Intest. Microbiol., 3, 29-38.
15. Rader, A. E. and Murphy, J. R. (1988) Nucleotide sequences and comparison of the hemolysin determinants of Vibrio cholerae El Tor RV79 (Hly+) and RV79 (Hly-) and Classical 569B (Hly-). Infect. Immun., 56, 1414-1419.
16. Rivera, I. N. G., Chun, J. S., Huq, A., Sack, R. B., and Colwell, R. R. (2001) Genotypes associated with virulence in environmental isolates of Vibrio cholerae. Appl. Environ. Microbiol., 67, 2424-2429.
17. Sack, D. A., Lyke, C., Mc Laughlin, C., and Suwanvanichkij, V. (2001) Antimicrobial resistance in shigellosis, cholera and campylobacteriosis. World Health Organ., 8, 8-20.
18. Salim, A., Lan, R., and Reeves, P. R. (2005) Vibrio cholerae pathogenic clones. Emerg. Infect. Dis., 11, 1758-1760.
19. Singh, D. V., Matte, M. H., Matte, G. R., Jiang, S., Sabeena, F., Shukla, B. N., Sanyal, S. C., Huq, A., and Cowell, R. R. (2001) Molecular analysis of Vibrio cholerae O1, O139, non-O1, and non-O139 strains: Clonal relationships between clinical and environmental isolates. Appl. Environ. Microbiol., 67, 910-921.
20. Teh, C. S. J., Chua, K. H., Puthucheary, S. D., and Thong, K. L. (2008) Further evaluation of a multiplex PCR for differentiation of Salmonella Parayphi A from other Salmonellae. Jpn. J. Infect. Dis., 61, 313-314.
21. Uma, G., Chandrasekaran, M., Takeda, Y., and Nair, G. B. (2003) Recent advances in cholera genetics. Curr. Sci., 85, 1538-1545.
22. World Health Organization. (2009) Outbreak News - Cholera Zimbabwe. Wkly. Epidemiol. Rec., 84(97), 50-52.