Bone morphogenetic protein-6-loaded chitosan scaffolds enhance the osteoblastic characteristics of MC3T3-E1 cells

Artificial Organs

Volumn 34, Issue 1, 2010, Pages 65-74

  Akman, Abdullah C ^a   Seda Tigli R ^b   Gumusderelioglu, Menemşe ^b   Nohutcu, Rahime M ^a  

^a Faculty of Dentistry   (Turkey)

^b HACETTEPE UNIVERSITY   (Turkey)

Author keywords

Bone morphogenetic protein 6; Bone tissue engineering; Chitosan; MC3T3 E1 cells; Scaffold

Indexed keywords

ASSAYS; BONE; CELL ENGINEERING; CELLS; CYTOLOGY; MINERALOGY; PHOSPHATASES; SCAFFOLDS (BIOLOGY); SCANNING ELECTRON MICROSCOPY;

BONE MORPHOGENETIC PROTEIN 6; BONE MORPHOGENETIC PROTEINS; BONE TISSUE ENGINEERING; CELL-BE; CELL/B.E; CHITOSAN SCAFFOLD; MC3T3-E1 CELL; MINERALIZATION ASSAYS; OSTEOCALCIN; PREOSTEOBLASTIC CELLS;

CHITOSAN;

ALKALINE PHOSPHATASE; BONE MORPHOGENETIC PROTEIN 6; CHITOSAN; OSTEOCALCIN; SCAFFOLD PROTEIN;

ANIMAL CELL; ARTICLE; ASSAY; BONE MINERALIZATION; BONE TISSUE; CELL PROLIFERATION; CELL STRAIN; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; EXTRACELLULAR MATRIX; MOUSE; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; TISSUE ENGINEERING;

EID: 75649142108     PISSN: 0160564X     EISSN: 15251594     Source Type: Journal    
DOI: 10.1111/j.1525-1594.2009.00798.x     Document Type: Article

References (46)

1. Salgado AJ, Coutinho OP, Reis RL. Bone tissue engineering: state of the art and future trends. Macromol Biosci 2004 4 : 743 765.
2. Mastrogiacomo M, Muraglia A, Komlev V, et al. Tissue engineering of bone: search for a better scaffold. Orthod Craniofac Res 2005 8 : 277 284.
3. Franceschi RT. Biological approaches to bone regeneration by gene therapy. J Dent Res 2005 84 : 1093 1103.
4. Logeart-Avramoglou D, Anagnostou F, Bizios R, Petite H. Engineering bone: challenges and obstacles. J Cell Mol Med 2005 9 : 72 84.
5. Huang KK, Shen C, Chiang CY, Hsieh YD, Fu E. Effects of bone morphogenetic protein-6 on periodontal wound healing in a fenestration defect of rats. J Periodontal Res 2005 40 : 1 10.
6. Meyer U, Joos U, Wiesmann HP. Biological and biophysical principles in extracorporal bone tissue engineering. Part III. Int J Oral Maxillofac Surg 2004 33 : 635 641. (Pubitemid 39186152)
7. Saito N, Takaoka K. New synthetic biodegradable polymers as BMP carriers for bone tissue engineering. Biomaterials 2003 24 : 2287 2293.
8. Weng Y, Wang M, Liu W, et al. Repair of experimental alveolar bone defects by tissue-engineered bone. Tissue Eng 2006 12 : 1503 1513.
9. Dunn CA, Jin Q, Taba M Jr., Franceschi RT, Bruce Rutherford R, Giannobile WV. BMP gene delivery for alveolar bone engineering at dental implant defects. Mol Ther 2005 11 : 294 299.
10. Yamada Y, Ueda M, Hibi H, Baba S. A novel approach to periodontal tissue regeneration with mesenchymal stem cells and platelet-rich plasma using tissue engineering technology: a clinical case report. Int J Periodontics Restorative Dent 2006 26 : 363 369.
11. Vacanti CA, Vacanti JP. The science of tissue engineering. Orthop Clin North Am 2000 31 : 351 356.
12. Dai KR, Xu XL, Tang TT, et al. Repairing of goat tibial bone defects with BMP-2 gene-modified tissue-engineered bone. Calcif Tissue Int 2005 77 : 55 61.
13. Ripamonti U, Renton L. Bone morphogenetic proteins and the induction of periodontal tissue regeneration. Periodontol 2000 2006 41 : 73 87.
14. Friedman MS, Long MW, Hankenson KD. Osteogenic differentiation of human mesenchymal stem cells is regulated by bone morphogenetic protein-6. J Cell Biochem 2006 98 : 538 554.
15. Seto I, Marukawa E, Asahina I. Mandibular reconstruction using a combination graft of rhBMP-2 with bone marrow cells expanded in vitro. Plast Reconstr Surg 2006 117 : 902 908.
16. Wiesmann HP, Joos U, Meyer U. Biological and biophysical principles in extracorporal bone tissue engineering. Part II. Int J Oral Maxillofac Surg 2004 33 : 523 530. (Pubitemid 39145174)
17. Simic P, Culej JB, Orlic I, et al. Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption. J Biol Chem 2006 281 : 25509 25521.
18. Bertone AL, Pittman DD, Bouxsein ML, Li J, Clancy B, Seeherman HJ. Adenoviral-mediated transfer of human BMP-6 gene accelerates healing in a rabbit ulnar osteotomy model. J Orthop Res 2004 22 : 1261 1270.
19. Di Martino A, Sittinger M, Risbud MV. Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. Biomaterials 2005 26 : 5983 5990.
20. Klokkevold PR, Vandemark L, Kenney EB, Bernard GW. Osteogenesis enhanced by chitosan (poly-N-acetyl glucosaminoglycan) in vitro. J Periodontol 1996 67 : 1170 1175.
21. Abarrategi A, Civantos A, Ramos V, Sanz Casado JV, Lopez-Lacomba JL. Chitosan film as rhBMP2 carrier: delivery properties for bone tissue application. Biomacromolecules 2008 9 : 711 718.
22. Lee JE, Kim SE, Kwon IC, et al. Effects of a chitosan scaffold containing TGF-beta1 encapsulated chitosan microspheres on in vitro chondrocyte culture. Artif Organs 2004 28 : 829 839.
23. Lee JY, Nam SH, Im SY, et al. Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials. J Control Release 2002 78 : 187 197.
24. Lee YM, Park YJ, Lee SJ, et al. The bone regenerative effect of platelet-derived growth factor-BB delivered with a chitosan/tricalcium phosphate sponge carrier. J Periodontol 2000 71 : 418 424.
25. Tiǧli RS, Karakecili A, Gümüşderelioǧlu M. In vitro characterization of chitosan scaffolds: influence of composition and deacetylation degree. J Mater Sci Mater Med 2007 18 : 1665 1674.
26. Grasser WA, Orlic I, Borovecki F, Riccardi KA, Simic P, Vukicevic S, et al. BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways. Int Orthop 2007 31 : 759 765.
27. Xu WP, Shiba H, Mizuno N, et al. Effect of bone morphogenetic proteins-4, -5 and -6 on DNA synthesis and expression of bone-related proteins in cultured human periodontal ligament cells. Cell Biol Int 2004 28 : 675 682.
28. Akman AC, Tiǧli RS, Gümüşderelioǧlu M, Nohutcu RM. bFGF loaded HA-chitosan: a promising scaffold for periodontal tissue engineering. J Biomed Mater Res A 2009 (in press).
29. Gerçek I, Tiǧli RS, Gümüşderelioǧlu M. A novel scaffold based on formation and agglomeration of PCL microbeads by freeze-drying. J Biomed Mater Res A 2008 86 : 1012 1022.
30. Ikada Y. Challenges in tissue engineering. J R Soc Interface 2006 3 : 589 601.
31. Sendemir-Urkmez A, Jamison RD. The addition of biphasic calcium phosphate to porous chitosan scaffolds enhances bone tissue development in vitro. J Biomed Mater Res A 2007 81 : 624 633.
32. Seol YJ, Lee JY, Park YJ, et al. Chitosan sponges as tissue engineering scaffolds for bone formation. Biotechnol Lett 2004 26 : 1037 1041.
33. VandeVord PJ, Matthew HW, DeSilva SP, Mayton L, Wu B, Wooley PH. Evaluation of the biocompatibility of a chitosan scaffold in mice. J Biomed Mater Res 2002 59 : 585 590.
34. Sudo H, Kodama HA, Amagai Y, Yamamoto S, Kasai S. In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol 1983 96 : 191 198.
35. Tiǧli RS, Gümüşderelioǧlu M. Chondrogenesis on BMP-6 loaded chitosan scaffolds in stationary and dynamic cultures. Project Report, Turkish Scientific and Research Council, Project no: 105M097, 2008.
36. Franceschi RT. The developmental control of osteoblast-specific gene expression: role of specific transcription factors and the extracellular matrix environment. Crit Rev Oral Biol Med 1999 10 : 40 57.
37. Ott SM. Histomorphometric analysis of bone remodeling. In : Bilezikian JP, Raisz LG, Rodan GA, eds. Principles of Bone Biology, 2nd Edition. San Diego : Academic Press, 2002 303 320.
38. Wilson TGJ, Kornman KS, eds. Fundamentals of Periodontics, 2nd Edition. Chicago : Quintessence Publishing, 1996.
39. Lian JB, Stein GS. Concepts of osteoblast growth and differentiation: basis for modulation of bone cell development and tissue formation. Crit Rev Oral Biol Med 1992 3 : 269 305.
40. Moursi AM, Damsky CH, Lull J, et al. Fibronectin regulates calvarial osteoblast differentiation. J Cell Sci 1996 109 : 1369 1380.
41. Takeuchi Y, Nakayama K, Matsumoto T. Differentiation and cell surface expression of transforming growth factor-beta receptors are regulated by interaction with matrix collagen in murine osteoblastic cells. J Biol Chem 1996 271 : 3938 3944.
42. Li JZ, Li H, Sasaki T, et al. Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat. Gene Ther 2003 10 : 1735 1743.
43. Murakami Y, Kojima T, Nagasawa T, Kobayashi H, Ishikawa I. Novel isolation of alkaline phosphatase-positive subpopulation from periodontal ligament fibroblasts. J Periodontol 2003 74 : 780 786.
44. McCauley LK, Koh AJ, Beecher CA, Cui Y, Decker JD, Franceschi RT. Effects of differentiation and transforming growth factor beta 1 on PTH/PTHrP receptor mRNA levels in MC3T3-E1 cells. J Bone Miner Res 1995 10 : 1243 1255.
45. Riminucci M, Bianco P. Building bone tissue: matrices and scaffolds in physiology and biotechnology. Braz J Med Biol Res 2003 36 : 1027 1036.
46. Ducy P, Schinke T, Karsenty G. The osteoblast: a sophisticated fibroblast under central surveillance. Science 2000 289 : 1501 1504.