Next-generation 9216-microwell cell arrays for high-content screening microscopy

BioTechniques

Volumn 47, Issue 4, 2009, Pages 877-878

  Reymann, Jürgen ^a   Beil, Nina ^a   Beneke, Jürgen ^a   Kaletta, Peter Paul ^b   Burkert, Klaus ^b   Erfle, Holger ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b Graffinity Pharmaceutical Design GmbH   (Germany)

Author keywords

Cell microarrays; High content screening microscopy; High throughput; Microwells; Reverse transfection

Indexed keywords

COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN; OLIGONUCLEOTIDE; POLO LIKE KINASE 1; SMALL INTERFERING RNA; BIOMATERIAL; GELATIN; GLASS; TITANIUM;

ARTICLE; BIOTECHNOLOGY; GENE CONTROL; GENETIC TRANSFECTION; HELA CELL; HIGH THROUGHPUT SCREENING; MAMMAL CELL; MICROARRAY ANALYSIS; MICROSCOPY; MICROWELL CELL ARRAY; CHEMISTRY; DNA MICROARRAY; ELECTRONICS; ENZYME SPECIFICITY; FEASIBILITY STUDY; GENETICS; HUMAN; METHODOLOGY; SCANNING PROBE MICROSCOPY;

MAMMALIA;

COATED MATERIALS, BIOCOMPATIBLE; FEASIBILITY STUDIES; GELATIN; GLASS; HELA CELLS; HUMANS; MICROSCOPY, SCANNING PROBE; MINIATURIZATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RNA, SMALL INTERFERING; SUBSTRATE SPECIFICITY; TITANIUM; TRANSFECTION;

EID: 75549085977     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113251     Document Type: Article

References (9)

1. Ziauddin, J. and D.M. Sabatini. 2001. Microarrays of cells expressing defined cDNAs. Nature 411:107-110.
2. Mousses, S., N.J. Caplen, R. Cornelison, D. Weaver, M. Basik, S. Hautaniemi, A.G. Elkahloun, R.A. Lotufo, et al. 2003. RNAi microarray analysis in cultured mammalian cells. Genome Res. 13:2341-2347.
3. Kumar, R., D.S. Conklin, and V. Mittal. 2003. High-throughput selection of effective RNAi probes for gene silencing. Genome Res. 13:2333-2340. (Pubitemid 37265999)
4. Erfle, H., J.C. Simpson, P.I.H. Bastiaens, and R. Pepperkok. 2004. siRNA cell arrays for high-content screening microscopy. BioTechniques 37:454-462. (Pubitemid 39180919)
5. Silva, J.M., H. Mizuno, A. Brady, R. Lucito, and G.J. Hannon. 2004. RNA interference microarrays: High-throughput loss-of-function genetics in mammalian cells. Proc. Natl. Acad. Sci. USA 101:6548-6552.
6. Baghdoyan, S., Y. Roupioz, A. Pitaval, D. Castel, E. Khomyakova, A. Papine, F. Soussaline, and X. Gidrol. 2004. Quantitative analysis of highly parallel transfection in cell microarrays. Nucleic Acids Res. 32:e77.
7. Neumann, B., M. Held, U. Liebel, H. Erfle, P. Rogers, R. Pepperkok, and J. Ellenberg. 2006. High-throughput RNAi screening by time-lapse imaging of live human cells. Nat. Methods 3:385-390.
8. Erfle, H., B. Neumann, U. Liebel, P. Rogers, M. Held, T. Walter, and J. Ellenberg. 2007. Reverse transfection on cell arrays for high content screening microscopy. Nat. Protocols 2:392-399.
9. Erfle, H., B. Neumann, P. Rogers, J. Bulkescher, J. Ellenberg, and R. Pepperkok. 2008. Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates. J. Biomol. Screen. 13:575-580.