Expression and amplification of glutamine synthetase gene endows HepG2 cells with ammonia-metabolizing activity for bioartificial liver support system

Enzyme and Microbial Technology

Volumn 35, Issue 6-7, 2004, Pages 519-524

  Omasa, Takeshi ^a   Yamanaka, Mitsugu ^a   Tanimura, Naoko ^a   Katakura, Yoshio ^a   Kishimoto, Michimasa ^a   Suga, Ken Ichi ^a   Enosawa, Shin ^b  

^a OSAKA UNIVERSITY   (Japan)

^b NATIONAL RESEARCH INSTITUTE FOR CHILD HEALTH AND DEVELOPMENT   (Japan)

Author keywords

Ammonia metabolizing activity; Bioartificial liver support system; CHO; Gene amplification; HepG2 cell

Indexed keywords

BIOARTIFICIAL LIVERS; GLUTAMINE SYNTHETASE (GS) GENES;

AMMONIA; CELLS; METABOLISM;

GENES;

AMMONIA; ENZYME INHIBITOR; GLUTAMATE AMMONIA LIGASE; METHIONINE SULFOXIMINE; VIRUS VECTOR;

ANIMAL CELL; BIOARTIFICIAL ORGAN; CELL LINE; CELL METABOLISM; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME MECHANISM; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC RECOMBINATION; HUMAN; HUMAN CELL; LIVER; NONHUMAN;

CUCUMBER MOSAIC VIRUS;

EID: 7544247636     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2004.08.027     Document Type: Conference Paper

References (35)

1. J. Rozga, and A.A. Demetriou Evolution and future perspectives ASAIO J 41 1995 831 837
2. I.M. Sauer, N. Obermeyer, D. Kardassis, T. Theruvath, and J.C. Gerlach Development of a hybrid liver support system Ann N Y Acad Sci 944 2001 308 319
3. W.S. Hu, J.R. Friend, F.J. Wu, T. Sielaff, M.V. Peshwa, and A. Lazar Development of a bioartificial liver employing xenogeneic hepatocytes Cytotechnology 23 1997 29 38
4. S.L. Nyberg, and S.P. Misra Hepatocyte liver-assist systems - a clinical update Mayo Clin Proc 73 1998 765 771
5. A.L. Friedman Why bioartificial liver support remains the Holy Grail ASAIO J 44 1998 241 243
6. J. Stange, and S. Mitzner Cell sources for bioartificial liver support Int J Artif Organs 19 1996 14 17
7. A.J. Strain, and J.M. Neuberger A bioartificial liver - state of the art Science 295 2002 1005 1009
8. Omasa T, Kishimoto M, Kawase M, Yagi K. An attempt at decision making in tissue engineering: reactor evaluation using the analytic hierarchy process (AHP). Biochemical Eng J 2004;20:173-9.
9. N.L. Sussman, and J.H. Killy Improved liver function following treatment with an extracorporeal liver assist device Artif Organs 17 1993 27 30
10. A.J. Ellis, R.D. Hughes, J.A. Wendon, J. Dunne, P.G. Langley, and J.H. Kelly Pilot-controlled trial of the extracorporeal liver assist device in acute liver failure Hepatology 24 1996 1446 1451
11. S. Enosawa, S. Suzuki, T. Kakefuda, and H. Amemiya Examination of 7-ethoxycoumarin deethylation and ammonia removal activities in 31 hepatocyte cell lines Cell Transplant 5 1996 S39 S40
12. L. Wang, J. Sun, L. Li, D. Mears, M. Horvat, and A.G. Sheil Comparison of porcine hepatocytes with human hepatoma (C3A) cells for use in a bioartificial liver support system Cell Transplant 7 1998 459 468
13. D.D. Mousseau, and R.F. Butterworth Current theories on the pathogenesis of hepatic encephalopathy Proc Soc Exp Biol Med 206 1994 329 344
14. H. Wakabayashi, Y. Kuwabara, H. Murata, K. Kobashi, and A. Watanabe Measurement of the expiratory ammonia concentration and its clinical significance Metab Brain Dis 12 1997 161 169
15. D. Haussinger Nitrogen metabolism in liver: structural and functional organization and physiological relevance Biochem J 267 1990 281 290
16. D. Haussinger Hepatocyte heterogeneity in glutamine and ammonia metabolism and the role of an intercellular glutamine cycle during ureogenesis in perfused rat liver Eur J Biochem 133 1983 269 275
17. M.I. Cockett, C.R. Bebbington, and G.T. Yarranton High level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplification Biotechnology 8 1990 662 667
18. T. Omasa Gene amplification and its application in cell and tissue engineering J Biosci Bioeng 94 2002 600 605
19. E.A. Conner, and P.J. Wirth Protein alterations associated with gene amplification in cultured human and rodent cells Electrophoresis 17 1996 1257 1264
20. T. Omasa, M. Ishimoto, K. Higashiyama, S. Shioya, and K. Suga The effects of glutamine concentration on growth and monoclonal antibody production in fed-batch operation Cytotechnology 8 1992 75 84
21. T. Omasa, K. Higashiyama, S. Shioya, and K. Suga Effect of lactate concentration on hybridoma culture in lactate-controlled fed-batch operation Biotechnol Bioeng 39 1992 556 564
22. W.B. Rowe, R.A. Ronzio, V.P. Wellener, and A. Meister Glutamine synthetase (sheep brain) Methods Enzymol 17a 1970 901 910
23. T. Yoshikawa, F. Nakanishi, Y. Ogura, D. Oi, T. Omasa, and Y. Katakura Amplified gene location in chromosomal DNA affected recombinant protein production and stability of amplified genes Biotechnol Prog 16 2000 710 715
24. N.E. Miller, and J. Halpert Analogues of chloramphenicol as mechanism-based inactivators of rat liver cytochrome P-450: modifications of the propanediol side chain, the p-nitro group, and the dichloromethyl moiety Mol Pharmacol 29 1986 391 398
25. P.B. Soeters, and J.E. Fischer Insulin, glucagon, amino acid imbalance, and hepatic encephalopathy Lancet 308 1976 880 882
26. Z. Chen, Y. Ding, Q. Xu, and D. Yu Bioartificial liver inoculated with porcine hepatocyte spheroids for treatment of canine acute liver failure model Artif Organs 27 2003 613 622
27. J. Denis, M.L. Delorme, M. Boschat, B. Nordlinger, and P. Opolon Respective roles of ammonia, amino acids, and medium-sized molecules in the pathogenesis of experimentally induced acute hepatic encephalopathy J Neurochem 40 1983 10 19
28. P. Reding, J. Duchateau, and C. Bataille Oral zinc supplementation improves hepatic encephalopathy Results of a randomised controlled trial Lancet 324 1984 493 495
29. Y. Yoshida, T. Higashi, K. Nouso, H. Nakatsukasa, S.I. Nakamura, and A. Watanabe Effects of zinc deficiency/zinc supplementation on ammonia metabolism in patients with decompensated liver cirrhosis Acta Med Okayama 55 2001 349 355
30. A.M. Edwards, M.L. Glistak, C.M. Lucas, and P.A. Wilson 7-Ethoxycoumarin deethylase activity as a convenient measure of liver drug metabolizing enzymes: regulation in cultured rat hepatocytes Biochem Pharmacol 33 1984 1537 1546
31. S. Enosawa, T. Miyashita, S. Suzuki, X.K. Li, M. Tsunoda, and H. Amemiya Long-term culture of glutamine synthetase-transfected HepG2 cells in circulatory flow bioreactor for development of a bioartificial liver Cell Transplant 9 2000 711 715
32. T. Miyashita, S. Enosawa, S. Suzuki, A. Tamura, H. Tanaka, and H. Amemiya Development of a bioartificial liver with glutamine synthetase-transduced recombinant human hepatoblastoma cell line HepG2 Transplant Proc 32 2000 2355 2358
33. S. Enosawa, T. Miyashita, Y. Fujita, S. Suzuki, H. Amemiya, and T. Omasa In vivo estimation of bioartificial liver with recombinant HepG2 cells using pigs with ischemic liver failure Cell Transplant 10 2001 429 433
34. S. Enosawa, T. Miyashita, H. Tanaka, X. Li, S. Suzuki, and H. Amemiya Prolongation of survival of pigs with ischemic liver failure by treatment with a bioartificial liver using glutamine synthetase transfected recombinant HepG2 Transplant Proc 33 2001 1945 1947
35. H. Ijima, T. Matsushita, and K. Funatsu Development of a hybrid type artificial liver using PUF/Sheroid culture system of adult hepatocytes Jpn J Artif Organs 22 1993 171 176