Campylobacter jejuni and Campylobacter coli genotyping by high-resolution melting analysis of a flaA fragment

Applied and Environmental Microbiology

Volumn 76, Issue 2, 2010, Pages 493-499

  Merchant Patel, Shreema ^a   Blackall, Patrick J ^b   Templeton, Jillian ^b   Price, Erin P ^a,d   Tong, Steven Y C ^c   Huygens, Flavia ^a   Giffard, Philip M ^a,c  

^a QUEENSLAND UNIVERSITY OF TECHNOLOGY   (Australia)

^b DEPARTMENT OF AGRICULTURE AND FISHERIES   (Australia)

^c MENZIES SCHOOL OF HEALTH RESEARCH   (Australia)

^d NORTHERN ARIZONA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; GENOTYPING; HIGH RESOLUTION; PCR PRIMERS; PCR PRODUCTS; RESOLVING POWER; RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS; ROBUST METHODS; SEQUENCE VARIANTS; SINGLE NUCLEOTIDE POLYMORPHISMS;

GENE ENCODING; POLYMORPHISM;

AMPLIFICATION;

FLAA PROTEIN, BACTERIA; FLAGELLIN;

BACTERIUM; DNA; GENE; GENETIC ANALYSIS; GENOTYPE; POLYMERASE CHAIN REACTION; POLYMORPHISM;

ARTICLE; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; GENETICS; GENOTYPE; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SINGLE NUCLEOTIDE POLYMORPHISM;

CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; FLAGELLIN; GENOTYPE; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; POLYMORPHISM, SINGLE NUCLEOTIDE;

CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI;

EID: 75249105299     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01164-09     Document Type: Article

References (34)

1. Alm, R. A., P. Guerry, and T. J. Trust. 1993. Significance of duplicated flagellin genes in Campylobacter. J. Mol. Biol. 230:359-363.
2. SHV codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis. Antimicrob. Agents Chemother. 53:2679-2683. doi: 10.1128/AAC.00063-09.
3. Erali, M., K. V. Voelkerding, and C. T. Wittwer. 2008. High resolution melting applications for clinical laboratory medicine. Exp. Mol. Pathol. 85: 50-58.
4. Ertas, H. B., B. Cetinkaya, A. Muz, and H. Ongor. 2004. Genotyping of broiler-originated Campylobacter jejuni and Campylobacter coli isolates using fla typing and random amplified polymorphic DNA methods. Int. J. Food Microbiol. 94:203-209.
5. Fearnhead, P., N. G. Smith, M. Barrigas, A. Fox, and N. French. 2005. Analysis of recombination in Campylobacter jejuni from MLST population data. J. Mol. Evol. 61:333-340.
6. Fitzgerald, C., L. O. Helsel, M. A. Nicholson, S. J. Olsen, D. L. Swerdlow, R. Flahart, J. Sexton, and P. I. Fields. 2001. Evaluation of methods for subtyping Campylobacter jejuni during an outbreak involving a food handler. J. Clin. Microbiol. 39:2386-2390.
7. Guerry, P. 2007. Campylobacter flagella: not just for motility. Trends Microbiol. 15:456-461.
8. Guerry, P. 2007. The role of flagella in Campylobacter virulence, p. 197-207. In K. A. Brogden, F. C. Minion, N. Cornick, T. B. Stanton, Q. Zhang, L. K. Nolan, and M. J'. Wannemuehler (ed.), Virulence mechanisms of bacterial pathogens, 4th ed. ASM Press, Washington, DC.
9. Guerry, P., S. M. Logan, S. Thornton, and T. J. Trust. 1990. Genomic organization and expression, of Campylobacter flagellin genes. J. Bacteriol. 172:1853-1860.
10. Jolley, K. A., and M. C. Maiden. 2006. AgdbNet - antigen sequence database software for bacterial typing. BMC Bioinformatics 7:314.
11. Keim, P., T. Pearson, and R. Okinaka. 2008. Microbial forensics: DNA fingerprinting of Bacillus anthracis (anthrax). Anal. Chem. 80:4791-4799.
12. Keim, P., M. N. Van Ert, T. Pearson, A. J. Vogler, L. Y. Huynh, and D. M. Wagner. 2004. Anthrax molecular epidemiology and forensics: using the appropriate marker for different evolutionary scales. Infect. Genet. Evol. 4:205-213.
13. Krypuy, M., G. M. Newnham, D. M. Thomas, M. Conron, and A. Dobrovic. 2006. High resolution melting analysis for the rapid and sensitive detection of mutations in clinical samples: KRAS codon 12 and 13 mutations in non-small cell lung cancer. BMC Cancer 6:295.
14. Lehmensiek, A., M. W. Sutherland, and R. B. McNamara. 2008. The use of high resolution melting (HRM) to map single nucleotide polymorphism markers linked to a covered smut resistance gene in barley. Theor. Appl. Genet. 11.7:721-728.
15. Maltese, P., E. Canestrari, L. Palma, A. Ruzzo, F. Corini, M. Menotta, F. Andreoni, A. Latiano, V. Annese, and M. Magnani. 2009. High resolution melting (HRM) analysis for the detection of ER22/23EK, Bell, and N363S polymorphisms of the glucocorticoid receptor gene. J. Steroid Biochem. Mol. Biol. 113:269-274.
16. Meinersmann, R. J., L. O. Helsel, P. I. Fields, and K. L. Hiett. 1997. Discrimination of Campylobacter jejuni isolates by fia gene sequencing. J. Clin. Microbiol. 35:2810-2814.
17. Merchant-Patel, S., P. J. Blackall, J. Templeton, E. P. Price, J. K. Miflin, F. Huygens, and P. M. Giffard. 2008. Characterisation of chicken Campylobacter jejuni isolates using resolution optimised single nucleotide polymorphisms and binary gene markers. Int. J. Food Microbiol. 128:304-308.
18. Miflin, J. K., J. M. Templeton, and P. J. Blackall. 2007. Antibiotic resistance in Campylobacter jejuni and Campylobacter coll isolated from poultry in the South-East Queensland region. J. Antimicrob. Chemother. 59:775-778.
19. Nachamkin, I., K. Bohachick, and C. M. Patton. 1993. Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J. Clin. Microbiol. 31:1531-1536.
20. Nuijten, P. J., F. J. van Asten, W. Gaastra, and B. A. van der Zeijst. 1990. Structural and functional analysis of two Campylobacter jejuni flagellin genes. J. Biol. Chem. 265:17798-17804.
21. Park, S. F. 2002. The physiology of Campylobacter species and its relevance to their role as foodborne pathogens. Int. J. Food Microbiol. 74:177-188.
22. Pittenger, L. G., M. D. Englen, C. T. Parker, J. G. Frye, B. Quinones, S. T. Horn, I. Son, P. J. Fedorka-Cray, and M. A. Harrison. 2009. Genotyping Campylobacter jejuni by comparative genome indexing: an evaluation with pulsed-field gel electrophoresis and flaA SVR sequencing. Foodborne Pathog. Dis. 6:337-649.
23. Price, E. P., F. Huygens, and P. M. Giffard. 2006. Fingerprinting of Campylobacter jejuni by using resolution-optimized binary gene targets derived from comparative genome hybridization studies. Appl. Environ. Microbiol. 72: 7793-7803.
24. Price, E. P., H. Smith, F. Huygens, and P. M. Giffard. 2007. High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni. Appl. Environ. Microbiol. 73: 3431-3436.
25. Price, E. P., V. Thiruvenkataswamy, L. Mickan, L. Unicomb, R. E. Rios, F. Huygens, and P. M. Giffard. 2006. Genotyping of Campylobacter jejuni using seven single-nucleotide polymorphisms in combination with flaA short variable region sequencing. J. Med. Microbiol. 55:1061-1070.
26. 26. Reed, G. H., J. O. Kent, and C. T. Wittwer. 2007. High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics 8:597-608. (Pubitemid 46939585)
27. Stephens, A. J., J. Inman-Bamber, P. M. Giffard, and F. Huygens. 2008. High-resolution melting analysis of the spa repeat region of Staphylococcus aureus, Clin. Chem. 54:432-436.
28. Suerbaum, S., M. Lohrengel, A. Sonnevend, F. Ruberg, and M. Kist. 2001. Allelic diversity and recombination in Campylobacter jejuni. J. Bacteriol. 183:2553-2559.
29. Szvetko, A. L., A. Jones, J. Mackenzie, L Tajouri, P. A. Csurhes, J. M. Greer, M. P. Pender, and L. R. Griffiths. 2009. An investigation of the C77G and C772T variations within the human protein tyrosine phosphatase receptor type C gene for association with multiple sclerosis in an Australian population. Brain Res. 1255:148-152.
30. Taylor, C. F. 2009. Mutation scanning using high-resolution melting. Biochem. Soc. Trans. 37:433-437.
31. Tong, S. Y., R. A. Lilliebridge, D. C. Holt, M. I. McDonald, B. J. Currie, and P. M. Giffard. 2009. High-resolution melting analysis of the spa locus reveals significant diversity within sequence type 93 methicillin- resistant Staphylococcus aureus from northern Australia. Clin. Microbiol. Infect. [Epub ahead of print.] doi:10.1111/j.1469-0691.2009.02732x.
32. Unicomb, L E., L. C. O'Reilly, M. D. Kirk, R. J. Stafford, H. V. Smith, N. G. Becker, M. S. Patel, and G. L. Gilbert. 2008. Risk factors for infection with Campylobacter jejuni flaA genotypes. Epidemiol. Infect. 136:1480-1491.
33. Wassenaar, T. M., and D. G. Newell. 2000. Genotyping of Campylobacter spp. Appl. Environ. Microbiol. 66:1-9.
34. Wilson, D. J., E. Gabriel, A. J. Leatherbarrow, J. Cheesbrough, S. Gee, E. Bolton, A. Fox, C. A. Hart, P. J. Diggle, and P. Fearnhead. 2009. Rapid evolution and the importance of recombination to the gastroenteric pathogen Campylobacter jejuni. Mol. Biol. Evol. 26:385-397.