EOS lentiviral vector selection system for human induced pluripotent stem cells

Nature Protocols

Volumn 4, Issue 12, 2009, Pages 1828-1844

  Hotta, Akitsu ^a,b   Cheung, Aaron Y L ^a,b,c   Farra, Natalie ^a,c   Garcha, Kamal ^b,d   Chang, Wing Y ^b,d   Pasceri, Peter ^a   Stanford, William L ^b,d   Ellis, James ^a,b,c  

^a Developmental and Stem Cell Biology Program   (Canada)

^b SickKids   (Canada)

^c UNIVERSITY OF TORONTO   (Canada)

^d UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGENASE; ENHANCED GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; OCTAMER TRANSCRIPTION FACTOR 4; PUROMYCIN; RETROVIRUS VECTOR; TRANSCRIPTION FACTOR SOX2;

ARTICLE; BIOSAFETY; CELL ISOLATION; CULTURE TECHNIQUE; FIBROBLAST; FREEZE THAWING; HUMAN; INTERNAL RIBOSOME ENTRY SITE; LONG TERMINAL REPEAT; NONVIRAL GENE DELIVERY SYSTEM; NUCLEAR REPROGRAMMING; PLASMID; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROMOTER REGION; TRANSPOSON; ULTRACENTRIFUGATION;

ANIMALS; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; DRUG RESISTANCE; FIBROBLASTS; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; LENTIVIRUS; MICE; TRANSFECTION;

EID: 74949128892     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.201     Document Type: Article

References (37)

1. Takahashi, K. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872 (2007).
2. Yu, J. et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917-1920 (2007).
3. Park, I.H. et al. Reprogramming of human somatic cells to pluripotency with defined factors. Nature 451, 141-146 (2008).
4. Takahashi, K., Okita, K., Nakagawa, M. & Yamanaka, S. Induction of pluripotent stem cells from fibroblast cultures. Nat. Protoc. 2, 3081-3089 (2007).
5. Maherali, N. & Hochedlinger, K. Guidelines and techniques for the generation of induced pluripotent stem cells. Cell Stem Cell 3, 595-605 (2008).
6. Ellis, J. et al. Alternative induced pluripotent stem cell characterization criteria for in vitro applications. Cell Stem Cell 4, 198-199 (2009).
7. Daley, G.Q. et al. Broader implications of defining standards for the pluripotency of iPSCs. Cell Stem Cell 4, 200-201 (2009).
8. Yamanaka, S. A fresh look at iPS cells. Cell 137, 13-17 (2009).
9. Lowry, W.E. et al. Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc. Natl. Acad. Sci. USA 105, 2883-2888 (2008).
10. Huangfu, D. et al. Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nat. Biotechnol. 26, 1269-1275 (2008).
11. Tiscornia, G., Singer, O. & Verma, I.M. Production and purification of lentiviral vectors. Nat. Protoc. 1, 241-245 (2006).
12. Hotta, A. et al. Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. Nat. Methods 6, 370-376 (2009).
13. Maksakova, I.A. & Mager, D.L. Transcriptional regulation of early transposon elements, an active family of mouse long terminal repeat retrotransposons. J. Virol. 79, 13865-13874 (2005).
14. Okumura-Nakanishi, S., Saito, M., Niwa, H. & Ishikawa, F. Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cells. J. Biol. Chem. 280, 5307-5317 (2005).
15. Tomioka, M. et al. Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex. Nucleic Acids Res. 30, 3202-3213 (2002).
16. Buzina, A. et al. Beta-globin LCR and intron elements cooperate and direct spatial reorganization for gene therapy. PLoS Genet. 4, e1000051 (2008).
17. Zufferey, R. et al. Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J. Virol. 72, 9873-9880 (1998).
18. Osborne, C.S. et al. Amelioration of retroviral vector silencing in locus control region beta-globin-transgenic mice and transduced F9 embryonic cells. J. Virol. 73, 5490-5496 (1999).
19. Nakagawa, M. et al. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat. Biotechnol. 26, 101-106 (2008).
20. Hotta, A. & Ellis, J. Retroviral vector silencing during iPS cell induction: an epigenetic beacon that signals distinct pluripotent states. J. Cell. Biochem. 105, 940-948 (2008).
21. Park, I.H., Lerou, P.H., Zhao, R., Huo, H. & Daley, G.Q. Generation of human-induced pluripotent stem cells. Nat. Protoc. 3, 1180-1186 (2008).
22. Ohnuki, M., Takahashi, K. & Yamanaka, S. Generation and characterization of human induced pluripotent stem cells. Curr. Protoc. Stem. Cell Biol. Chapter 4, Unit 4A.2 (2009).
23. Soldner, F. et al. Parkinson's disease patient-derived induced pluripotent stem cells free of viral reprogramming factors. Cell 136, 964-977 (2009).
24. Woltjen, K. et al. piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature 458, 766-770 (2009).
25. Kaji, K. et al. Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature 458, 771-775 (2009).
26. Yu, J. et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science 324, 797-801 (2009).
27. Kim, D. et al. Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell 4, 472-476 (2009).
28. Ding, S. et al. Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell 122, 473-483 (2005).
29. Hotta, A. et al. Characterization of transient expression system for retroviral vector production. J. Biosci. Bioeng. 101, 361-368 (2006).
30. Burns, J.C., Friedmann, T., Driever, W., Burrascano, M. & Yee, J.K. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc. Natl. Acad. Sci. USA 90, 8033-8037 (1993).
31. Sastry, L., Johnson, T., Hobson, M.J., Smucker, B. & Cornetta, K. Titering lentiviral vectors: comparison of DNA, RNA and marker expression methods. Gene Ther. 9, 1155-1162 (2002).
32. Villegas, J. & McPhaul, M. Establishment and culture of human skin fibroblasts. Curr. Protoc. Mol. Biol. Chapter 28, Unit 28.23 (2005).
33. Morita, S., Kojima, T. & Kitamura, T. Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther. 7, 1063-1066 (2000).
34. Campbell, R.E. et al. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. USA 99, 7877-7882 (2002).
35. Bryja, V., Bonilla, S. & Arenas, E. Derivation of mouse embryonic stem cells. Nat. Protoc. 1, 2082-2087 (2006).
36. Michalska, A. Isolation and propagation of mouse embryonic fibroblasts and preparation of mouse embryonic feeder layer cells. Curr. Protoc. Stem. Cell. Biol. Chapter 1, Unit 1C 3 (2007).
37. Lifson, J.D., Sasaki, D.T. & Engleman, E.G. Utility of formaldehyde fixation for flow cytometry and inactivation of the AIDS associated retrovirus. J. Immunol. Methods 86, 143-149 (1986).