Rational introduction of disulfide bond to enhance optimal temperature of Lipomyces starkeyi α-dextranase expressed in Pichia pastoris

Journal of Microbiology and Biotechnology

Volumn 19, Issue 12, 2009, Pages 1506-1513

  Chen, Lin ^a   Yu, Chao ^a   Zhou, Xiangshan ^a   Zhang, Yuanxing ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Disulfide bond; Lipomyces starkeyi dextranase; Pichia pastoris; Site directed mutagenesis; Thermostability

Indexed keywords

DEXTRANASE; GLYCOSIDASE;

ARTICLE; ASCOMYCETES; CATALYSIS; CONTROLLED STUDY; DISULFIDE BOND; ENZYME KINETICS; LIPOMYCES STARKEYI; NONHUMAN; PENICILLIUM; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

AMINO ACID SUBSTITUTION; CYSTEINE; DEXTRANASE; DISULFIDES; ENZYME STABILITY; INDUSTRIAL MICROBIOLOGY; LIPOMYCES; MUTAGENESIS, SITE-DIRECTED; PICHIA; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TEMPERATURE;

LIPOMYCES STARKEYI; PENICILLIUM MINIOLUTEUM; PICHIA PASTORIS;

EID: 74749107692     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0902.0096     Document Type: Article

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