Flow cytometric analysis of the cadmium-exposed green alga Chlamydomonas reinhardtii (chlorophyceae)

European Journal of Phycology

Volumn 44, Issue 4, 2009, Pages 541-550

  Jamers, An ^a   Lenjou, Marc ^a   Deraedt, Pieter ^a   van Bockstaele, Dirk ^a   Blust, Ronny ^a   de Coen, Wim ^a  

^a UNIVERSITY OF ANTWERP   (Belgium)

Author keywords

Algae; Cadmium; Esterase activity; Flow cytometry; Oxidative stress; Toxicity

Indexed keywords

AMMONIUM COMPOUND; BIOACCUMULATION; CADMIUM; ECOTOXICOLOGY; ENZYME ACTIVITY; ESTER; FLOW CYTOMETRY; GREEN ALGA; MICROALGA; OXIDATION; PHYSIOLOGICAL RESPONSE; POLLUTION EXPOSURE; ULTRASTRUCTURE;

ALGAE; CHLAMYDOMONAS REINHARDTII; CHLOROPHYCEAE; CHLOROPHYTA;

EID: 74749088089     PISSN: 09670262     EISSN: 14694433     Source Type: Journal    
DOI: 10.1080/09670260903118214     Document Type: Article

References (50)

1. ADLER, N.E., SCHMITT-JANSEN, M. & ALTENBURGER, R. (2007). Flow cytometry as a tool to study phytotoxic modes of action. Environ. Toxicol. Chem., 26: 297-306.
2. BERTIN, G. & AVERBECK, D. (2006). Cadmium: cellular effects, modifications of biomolecules, modulation of DNA repair and genotoxic consequences. Biochimie, 88: 1549-1559.
3. BROWN, M. & WITTWER, C. (2000). Flow cytometry: principles and clinical applications in hematology. Clin. Chem., 46: 1221-1229.
4. CID, A., FIDALGO, P., HERRERO, C. & ABALDE, J. (1996). Toxic action of copper on the membrane system of a marine diatom measured by flow cytometry. Cytometry, 25: 32-36.
5. DORSEY, J., YENTSCH, C.M., MAYO, S. & MCKENNA, C. (1989). Rapid analytical technique for the assessment of cell metabolic activity in marine microalgae. Cytometry, Sep: 10: 622-628.
6. EL-SHEEKH, M.M., EL-NAGGAR, A.H., OSMAN, M.E.H. & EL-MAZALY, E. (2007). Effect of cobalt on growth, pigments and photosynthetic electron transport on Monoraphidium minutum and Nitzchia perminuta. Braz. J. Plant Physiol., 15: 159-166.
7. EMMENDORFFER, A., HECHT, M., LOHMANN-MATTHES, M.L. & ROESLER, J. (1991). A fast and easy method to determine the production of reactive oxygen intermediates by human and muring phagocytes using dihydrorhodamine 123. J. Immunol. Methods, 138: 133-135.
8. FLORYK, D. & HOUSTEK, J. (1999). Tetramethyl rhodamine methyl ester (TMRM) is suitable for cytofluorometric measurements of mitochondrial membrane potential in cells treated with digitonin. Bioscience Rep., 19: 27-34.
9. FRANKLIN, N.M., ADAMS, M.S., STAUBER, J.L. & LIM, R.P. (2001a). Development of an improved rapid enzyme inhibition bioassay with marine and freshwater microalgae using flow cytometry. Arch. Environ. Con. Tox., 40: 469-480.
10. FRANKLIN, N.M., STAUBER, J.L. & LIM, R.P. (2001b). Development of flow-cytometry based algal bioassays for assessing toxicity of copper in natural waters. Environ. Toxicol. Chem., 20: 160-170.
11. GASPAR, M.L., CABELLO, M.N., POLLERO, R. & AON, M.A. (2001). Fluorescein diacetate hydrolysis as a measure of fungal biomass in soil. Curr. Microbiol., 42: 339-344.
12. GUSTAFSSON, J.P. (2005). Visual Minteq v2.32. Royal Institute of Technology, Stockholm, Sweden.
13. HARRIS, E.H. (1989). The Chlamydomonas Sourcebook: a Comprehensive Guide. New York, USA: Academic Press.
14. HASSOUN, E.A. & STOHS, S.J. (1996). Cadmium-induced production of superoxide anion and nitric oxide, DNA single strand breaks and lactate dehydrogenase leakage in J774A.1 cell cultures. Toxicology, 112: 219-226.
15. HEALEY, F.P. & MEYERS, J. (1971). The Kok effect in Chlamydomonas reinhardtii. Plant Physiol., 47: 373-379.
16. JONKER, R.R., MEULEMANS, J.T., DUBELAAR, G.B.J., WILKINS, M.F. & RINGELBERG, J. (1995). Flow cytometry: a powerful tool in analysis of biomass distributions in phytoplankton. Water Sci. Technol., 32: 177-182.
17. KUPPER, H., KUPPER, F. & SPILLER, M. (1996). Environmental relevance of heavy metal-substituted chlorophylls using the example of water plants. J. Exp. Bot., 47: 259-266.
18. LEGENDRE, L., COURTIES, C. & TROUSSELLIER, M. (2001). Flow cytometry in oceanography 1989-1999: environmental challenges and research trends. Cytometry, 44: 164-172.
19. MARTIN-GONZALES, A., DIAZ, S., BORNIQUEL, S., GALLEGO, A. & GUTIERREZ, J.C. (2006). Cytotoxicity and bioaccumulation of heavy metals by ciliated protozoa isolated from urban wastewater treatment plants. Res. Microbiol., 157: 108-118.
20. MEI, L., HU, C., ZHU, Q., CHEN, L., KONG, Z. & LIU, Z. (2005). Copper and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in the microalga Pavlova viridis (Prymnesiophyceae). Chemosphere, 62: 565-572.
21. MODEL, M.A., KUKURUGA, M.A. & TODD, R.F. (1997). A sensitive flow cytometric method for measuring the oxidative burst. J. Immunol. Methods, 202: 105-111.
22. NASSIRI, Y., MANSOT, J.L., WERY, J., GINSBURGER-VOGEL, T. & AMIARD, J.C. (1997). Ultrastructural and electron energy loss spectroscopy studies of sequestration mechanisms of Cd and Cu in the marine diatom Skeletonema costatum. Arch. Environ. Con. Tox., 33: 147-155.
23. OLSON, R.J., FRANKEL, S.L., CHISHOLM, S.W. & SHAPIRO, H. (1983). An inexpensive flow cytometer for the analysis of fluorescence signals in phytoplankton: chlorophyll and DNA distributions. J. Exp. Mar. Biol. Ecol., 68: 129-144.
24. OLSON, R.J., VAULOT, D. & CHISHOLM, S.W. (1985). Marine phytoplankton distributions measured using shipboard flow cytometry. Deep-Sea Res. A, 32: 1273-1280.
25. OLSON, R.J., ZETTLER, E.R. & ANDERSON, O.K. (1989). Discrimination of eukaryotic phytoplankton cell types from light scatter and autofluorescence properties measured by flow cytometry. Cytometry, 10: 636-643.
26. OVERNELL, J. (1975). The effects of heavy metals on photosynthesis and loss of cell potassium in two species of marine algae, Dunaliella tertiolecta and Phaeodactylum tricornutum. Mar. Biol., 29: 99-103.
27. PERALES-VELA, H.V., GONZALEZ-MORENO, S., MONTES-HORCASITAS, C. & CANIZARES-VILLANUEVA, R.O. (2007). Growth, photosynthetic and respiratory responses to sub-lethal copper concentrations in Scenedesmus incrassatulus (Chlorophyceae). Chemosphere, 67: 2274-2281.
28. PLASEK, J. & SIGLER, K. (1996). Slow fluorescent indicators of membrane potential: a survey of different approaches to probe response analysis. J. Photochem. Photobiol. B: Biol., 33: 101-124.
29. PRASAD, M.N.V., DREJ, K., SKAWINSKA, A. & STRATKA, K. (1998). Toxicity of cadmium and copper in Chlamydomonas reinhardtii Wild-Type (WT 2137) and cell wall deficient mutant strain (CW 15). B. Environ. Contam. Tox., 60: 306-311.
30. REIRIZ, S., CID, A., TORRES, E., ABALDE, J. & HERRERO, C. (1994). Different responses of the marine diatom Phaeodactylum tricornutum to copper toxicity. Microbiologia, 10: 263-272.
31. RIOBOO GONZALEZ, O., HERRERO, C. & CID, A (2002). Physiological response of freshwater microalga (Chlorella vulgaris) to triazine and phenylurea herbicides. Aquatic Tox., 59: 225-235.
32. RITCHIE, R.J. (1982). Comparison of a lipophilic cation and microelectrodes to measure membrane potentials of the giant-celled algae, Chara australis (Charophyta) and Griffithsia monilis (Rhodophyta). Membrane Biol., 69: 57-63.
33. RITCHIE, R.J. (1999). Tetraphenylphosphonium cation (TPP{thorn}) is not a reliable membrane potential probe in the cyanobacterium Synechococcus R-2 PCC 7942. New Phytol., 141: 387-399.
34. 0-dichlorofluorescin and dihydrorhodamine 123 as fluorescent probes for intracellular H2O2 in cultured endothelial cells. Arch. Biochem. Biophys., 302: 348-355.
35. SCADUTO, JR., GROTYOHANN, R.S. (1999). Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives. Biophys. J., 76: 469-477.
36. SCHNURER, J. & ROSWALL, T. (1982). Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter. Appl. Environ. Microbiol., 43: 1256-1261.
37. SMITH, R.M., MARTELL, A.E. & MOTEKAITIS, R.J. (2005). NIST Critically Selected Stability Constants of Metal Complexes Database, NIST Standard Reference Database.
38. SZAKACS, G., JAKAB, K., ANTAL, F. & SARKADI, B. (1998). Diagnostics of multidrug resistance in cancer. Pathol. Oncol. Res., 4: 251-257.
39. TRASK, B.J., VAN DEN ENGH, G.J. & ELGERSHUIZEN, J.H. (1982). Analysis of phytoplankton by flow cytometry. Cytometry, 2: 258-264.
40. VEAL, D.A., DEERE, D., FERRARI, B., PIPER, J. & ATTFIELD, P.V. (2000). Fluorescence staining and flow cytometry for monitoring microbiol cells. J. Immunol. Methods, 243: 191-210.
41. VISVIKI, I. & RACHLIN, J.W. (1992). Ultrastructural changes in Dunaliella minuta following acute and chronic exposure to copper and cadmium. Arch. Environ. Con. Tox., 23: 420-425.
42. VISVIKI, I. & RACHLIN, R.W. (1994). Acute and chronic exposure of Dunaliella salina and Chlamydomonas bullosa to copper and cadmium: effects of ultrastructure. Arch. Environ. Con. Tox., 26: 154-162.
43. VOWELLS, S.J., SEKHSARIA, S., MALECH, H.L., SHALIT, M. & FLEISHER, T.A. (1995). Flow cytometric analysis of the granulocyte respiratory burst: a comparison study of fluorescent probes. J. Immunol. Methods, 178: 89-97.
44. WALRAND, S., VALEIX, S., RODRIGUEZ, C., LIGOT, P., CHASSAGNE, J. & VASSON, M.P. (2003). Flow cytometry study of polymorphonuclear neutrophil oxidative burst: a comparison of three fluorescent probes. Clin. Chim. Acta, 331: 103-110.
45. WATANABE, M., HENMI, K., OGAWA, K. & SUZUKI, T. (2003). Cadmium-dependent generation of reactive oxygen species and mitochondrial DNA breaks in photosynthetic and non-photosynthetic strains of Euglena gracilis. Comp. Biochem. Phys. Part C, 134: 227-234.
46. WATANABE, M. & SUZUKI, T. (2001). Cadmium-induced abnormality in strains of Euglena gracilis: morphological alteration and its prevention by zinc and cobalamin. Comp. Biochem. Phys. Part C, 130: 29-39.
47. WONG, A. & CORTOPASSI, G.A. (2002). High-throughput measurement of mitochondrial membrane potential in a neural cell line using a fluorescence plate reader. Biochem. Biophys. Res. Comm., 298: 750-754.
48. YENTSCH, C.M., HORAN, P.K., MUIRHEAD, K., DORTCH, Q., HAUGEN, E.M., LEGENDRE, L., MURPHY, L.S., PHINNEY, D., POMPONI, S.A., SPINRAD, R.W., WOOD, A.M., YENTSCH, C.S. & ZAHURENCE, B.J. (1983). Flow cytometry and sorting: a powerful technique with potential applications in aquatic sciences. Limnol. Oceanogr., 28: 1275-1280.
49. YU, Y., KONG, F., WANG, M., QIAN, L. & SHI, X. (2007). Determination of short-term copper toxicity in a multispecies microalgal population using flow cytometry. Ecotox. Environ. Safe., 66: 49-56.
50. ZHANG, H., HUANG, H.M., CARSON, R.C., MAHMOOD, J., THOMAS, H.M. & GIBSON, G.E. (2001). Assessment of membrane potentials of mitochondrial populations in living cells. Anal. Biochem., 298: 170-180.