A new method of quantifying glutathione levels in freshly isolated single superfused rat cardiomyocytes

Journal of Pharmacological and Toxicological Methods

Volumn 50, Issue 3, 2004, Pages 215-222

  King, N ^a   Korolchuk, S ^a   McGivan, J D ^a   Suleiman, M S ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

CellTracker Blue CMAC; Fluorescence microscopy; Ischaemia and reperfusion injury; New method of quantifying GSH; Rat; Single isolated and superfused cardiomyocytes

Indexed keywords

BROMOBIMANE; CARBON; CYANIDE; GLUTATHIONE; GLUTATHIONE TRANSFERASE; HYDROGEN PEROXIDE; MONOCHLOROBIMANE; SUCROSE; THIOL DERIVATIVE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANIMAL CELL; AQUEOUS SOLUTION; ARTICLE; BIOLOGICAL MONITORING; CALCIUM CELL LEVEL; CELL ISOLATION; CELL LEVEL; CELL VOLUME; CONCENTRATION RESPONSE; CONTROLLED STUDY; CORRELATION ANALYSIS; DARKNESS; DEVICE; EXPOSURE; FLUORESCENCE ANALYSIS; FLUORESCENCE MICROSCOPE; HEART MUSCLE CELL; INCUBATION TIME; MATHEMATICAL COMPUTING; MOLECULAR PROBE; MOUSE; NONHUMAN; OXIDATIVE STRESS; PH MEASUREMENT; PHOTOCHEMICAL QUENCHING; ROOM TEMPERATURE; SENSITIVITY ANALYSIS; STANDARD; STATISTICAL SIGNIFICANCE;

ANIMALS; CELL FUSION; CELL SEPARATION; CELL SIZE; CYANIDES; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME INHIBITORS; GLUTATHIONE; HYDROGEN PEROXIDE; MALE; MICROSCOPY, FLUORESCENCE; MYOCYTES, CARDIAC; OXIDANTS; OXIDATIVE STRESS; RATS; RATS, WISTAR; STAINING AND LABELING;

EID: 7444220127     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vascn.2004.05.003     Document Type: Article

References (28)

1. Blaustein A., Deneke S.M., Stolz R.I., Baxter D., Healey N., Fanburg B.L. Myocardial glutathione depletion impairs recovery after short periods of ischaemia. Circulation. 80:1989;1449-1457
2. Bond J.M., Herman B., Lemasters J.J. Recovery of cultured rat neonatal myocytes from hypercontracture after chemical hypoxia. Research Communications in Chemical Pathology and Pharmacology. 71:1991;195-208
3. Boyett M.R., Frampton J.E., Kirby M.S. The length, width, and volume of isolated rat and ferret ventricular myocytes during twitch contractions and changes in osmotic strength. Experimental Physiology. 76:1991;259-270
4. Briviba K., Fraser G., Sies H., Ketterer B. Distribution of the monochlorobimane conjugate between nucleus and cytosol in isolated hepatocytes. Biochemical Journal. 294:1993;631-633
5. Ceconi C., Bernocchi P., Boraso A., Cargnoni A., Pepi P., Curello S., Ferrari R. New insights on myocardial pyridine nucleotides and thiol redox state in ischemia and reperfusion damage. Cardiovascular Research. 47:2000;586-594
6. Cook J.A., Iype S.N., Mitchell J.B. Differential specificity of monochlorobimane for isozymes of human and rodent glutathione S-transferases. Cancer Research. 51:1991;1606-1612
7. Griffiths E.J., Lin H., Suleiman M.-S. NADH fluorescence in isolated guinea-pig and rat cardiomyocytes exposed to low or high stimulation rates and effect of metabolic inhibition with cyanide. Biochemical Pharmacology. 56:1998;173-179
8. Halestrap A.P., McGivan J.D. Measurement of membrane transport phenomena. Kornberg H.L., Metcalfe J.C., Northcote D.H., Pogson C.I., Tipton K.F. Techniques in Metabolic Research. 1979;1-23 Elsevier, Amsterdam
9. 2+ concentration of guinea pig heart. Cardiovascular Research. 23:1989;767-773
10. Huster D., Reichenbach A., Reichelt W. The glutathione content of retinal Müller (glial) cells: Effect of pathological conditions. Neurochemistry International. 36:2000;461-469
11. Josephson R.A., Silverman H.S., Lakatta E.G., Stern M.D., Zweier J.L. Study of the mechanisms of hydrogen peroxide and hydroxyl free radical-induced cellular injury and calcium overload in cardiac myocytes. Journal of Biological Chemistry. 266:1991;2354-2361
12. King N., Suleiman M.-S. Characteristics of L-alanine transport in cardiac sarcolemmal vesicles and into isolated cardiac myocytes. Pflügers Archiv. 436:1998;384-390
13. King N., McGivan J.D., Griffiths E.J., Halestrap A.P., Suleiman M.-S. Glutamate loading protects freshly isolated and perfused adult cardiomyocytes against intracellular ROS generation. Journal of Molecular and Cellular Cardiology. 35:2003;975-984
14. Kruh G.D., Zeng H., Rea P.A., Liu G., Chen Z.-S., Lee K., Belinsky M.G. MRP subfamily transporters and resistance to anticancer agents. Journal of Bioenergetics and Biomembranes. 33:2001;493-501
15. Kurtz J., Van der Veen I.T., Christen M. Fluorescent vital labelling to track cestaodes in a Copepod intermediate host. Experimental Parasitology. 100:2002;36-43
16. Le C.T., Hollaar L., Van der Valk E.J.M., Van der Laarse A. Buthionine sulphoximine reduces the protective capacity of myocytes to withstand peroxide-derived free radical attack. Journal of Molecular and Cellular Cardiology. 25:1993;519-528
17. Li S., Li X., Rozanski G.J. Regulation of glutathione in cardiac myocytes. Journal of Molecular and Cellular Cardiology. 35:2003;1145-1152
18. Lu S.C. Regulation of hepatic glutathione synthesis: Current concepts and controversies. FASEB Journal. 13:1999;1169-1183
19. Meister A., Anderson M.E. Glutathione. Annual Reviews of Biochemistry. 52:1983;711-760
20. Okada H., Tsuboi H., Esato K. The addition of glutathione (YM737) to a crystalloid cardioplegic solution enhances myocardial protection. Surgery Today. 24:1994;241-246
21. Rosser B.G., Powers S.P., Gores G.J. Calpain activity increases in hepatocytes following addition of ATP. Demonstration by a novel fluorescent approach. Journal of Biological Chemistry. 268:1993;23593-23600
22. Sebastia J., Cristófol R., Martín M., Rodríguez- Farré E., Sanfeliu C. Evaluation of fluorescent dyes for measuring intracellular glutathione content in primary cultures of human neurons and neuroblastoma SH-SY5Y. Cytometry. 51A:2003;16-25
23. Seiler K.S., Kehrer J.P., Starnes J.W. Exogenous glutathione attenuates stunning following intermittent hypoxia in isolated rat hearts. Free Radicals Research. 24:1996;115-122
24. Singh A., Lee K.J., Lee C.Y., Goldfarb R.D., Tsan M.-F. Relation between myocardial glutathione content and extent of ischaemia-reperfusion injury. Circulation. 80:1989;1795-1804
25. Suleiman M.-S., Halestrap A.P., Griffiths E.J. Mitochondria: A target for myocardial protection. Pharmacology and Therapeutics. 89:2001;29-46
26. Tauskela J.S., Hewitt K., Kang L.-P., Comas T., Gendron T., Hakim A., Hogan M., Durkin J., Morley P. Evaluation of glutathione-sensitive fluorescent dyes in cortical culture. Glia. 30:2000;329-341
27. Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidised glutathione: Applications to mammalian blood and other tissues. Analytical Biochemistry. 27:1969;502-522
28. Zweier J.L. Measurement of superoxide-derived free radicals in the reperfused heart. Journal of Biological Chemistry. 263:1988;1353-1357