Preparation and culture of limb blastema stem cells from regenerating larval and adult salamanders

Cold Spring Harbor Protocols

Volumn 5, Issue 1, 2010, Pages

  Kumar, Anoop ^a   Godwin, James W ^a,b  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b MONASH UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 74349130352     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5367     Document Type: Article

References (9)

1. Brown R, Brockes JP. 1991. Identification and expression of a regeneration-specific homeobox gene in the newt limb blastema. Development 111: 489-496.
2. Carlson MR, Bryant SV, Gardiner DM. 1998. Expression of Msx-2 during development, regeneration, and wound healing in axolotl limbs. J Exp Zool 282: 715-723.
3. Ferretti P, Brockes JP. 1991. Cell origin and identity in limb regeneration and development. Glia 4: 214-224.
4. Iten LE, Bryant SV. 1973. Forelimb regeneration from different levels of amputation in the newt N. viridescens. Length, rate and stages. Wilhem Roux Arch Dev Biol 173: 263-282.
5. Koshiba K, Kuroiwa A, Yamamoto H, Tamura K, Ide H. 1998. Expression of Msx genes in regenerating and developing limbs of axolotl. J Exp Zool 282: 703-714.
6. Kumar A, Velloso CP, Imokawa Y, Brockes JP. 2004. The regenerative plasticity of isolated urodele myofibers and its dependence on Msx1. PLoS Biol 2: e218. doi: 10.1371/journal.pbio.0020218.
7. Kumar A, Godwin JW, Gates PB, Garza-Garcia AA, Brockes JP. 2007. Molecular basis for the nerve dependence of limb regeneration in an adult vertebrate. Science 318: 772-777.
8. Morais da Silva S, Gates PB, Brockes JP. 2002. The newt ortholog of CD59 is implicated in proximodistal identity during amphibian limb regeneration. Dev Cell 3: 547-555.
9. Voss SR, Epperlein HH, Tanaka EM. 2009. Ambystoma mexicanum, the axolotl: A versatile amphibian model for regeneration, development, and evolution studies. Cold Spring Harb Protoc doi: 10.1101/ pdb.emo128.