Unexpected oligomeric structure of the FocA formate channel of Escherichia coli: A paradigm for the formate-nitrite transporter family of integral membrane proteins

FEMS Microbiology Letters

Volumn 303, Issue 1, 2010, Pages 69-75

  Falke, Dörte ^a   Schulz, Kristin ^a   Doberenz, Claudia ^a   Beyer, Lydia ^a   Lilie, Hauke ^a   Thiemer, Barbara ^a   Sawers, Robert Gary ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Fermentation; FNT family; Formate; Membrane protein; Pyruvate formate lyase

Indexed keywords

BETA GALACTOSIDASE; FORMIC ACID; GENOMIC DNA; LYASE; MEMBRANE PROTEIN; NITRITE; PYRUVIC ACID; CARRIER PROTEIN; ESCHERICHIA COLI PROTEIN; FOCA PROTEIN, E COLI; FORMIC ACID DERIVATIVE;

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIUM CULTURE; CIRCULAR DICHROISM; ENTEROBACTERIACEAE; ENZYME ACTIVITY; ENZYME ASSAY; ESCHERICHIA COLI; FERMENTATION; FOCA GENE; IMMUNOBLOTTING; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN STRUCTURE; ULTRAVIOLET SPECTROSCOPY; CHEMISTRY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR WEIGHT; PROCEDURES; PROTEIN MULTIMERIZATION; PROTEIN QUATERNARY STRUCTURE; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE;

ESCHERICHIA COLI;

CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FORMATES; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR WEIGHT; NITRITES; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY;

EID: 74349124142     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2009.01862.x     Document Type: Article

References (30)

1. Begg YA, Whyte JN Haddock BA (1977) The identification of mutants of Escherichia coli deficient in formate dehydrogenase and nitrate reductase activities using dye indicator plates. FEMS Microbiol Lett 2 : 47 50.
2. Böck A, King PW, Blokesch M Posewitz MC (2006) Maturation of hydrogenases. Adv Microb Physiol 51 : 1 71.
3. Böhm G, Muhr R Jaenicke R (1992) Quantitative analysis of protein far UV circular dichroism spectra by neural networks. Protein Eng 5 : 191 195.
4. Casadaban MJ Cohen SN (1979) Lactose genes fused to exogenous promoters in one step using Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. P Natl Acad Sci USA 76 : 4530 4533.
5. Clegg S, Yu F, Griffiths L Cole JA (2002) The roles of the polytopic membrane proteins NarK, NarU and NirC in Escherichia coli K-12: two nitrate and three nitrite transporters. Mol Microbiol 44 : 143 155.
6. Delomenie C, Foti E, Floch E, Diderot V, Porquet D, Dupuy C Bonaly J (2007) A new homolog of FocA transporters identified in cadmium-resistant Euglena gracilis. Biochem Bioph Res Co 358 : 455 461.
7. Forzi L Sawers RG (2007) Maturation of [NiFe]-hydrogenases in Escherichia coli. BioMetals 20 : 567 578.
8. Hormann K Andreesen JR (1994) Purification and characterization of a pyrrole-2-carboxylate oxygenase from Arthrobacter strain Py1. Biol Chem H-S 375 : 211 218.
9. Ito K (1984) Identification of secY (prlA) gene product involved in protein export in Escherichia coli. Mol Gen Genet 197 : 204 208.
10. Jia W Cole JA (2005) Nitrate and nitrite transport in Escherichia coli. Biochem Soc T 33 : 159 161.
11. Jia W, Tovell N, Clegg S, Trimmer M Cole J (2009) A single channel for nitrate uptake, nitrite export and nitrite uptake by Escherichia coli NarU and a role for NirC in nitrite export and uptake. Biochem J 417 : 297 304.
12. Kessler D Knappe J (1996) Anaerobic dissimilation of pyruvate. Escherichia coli and Salmonella: cellular and Molecular Biology (Neidhardt FC et al., eds), pp. 199 205. ASM Press, Washington, DC.
13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 : 680 685.
14. Lowry OH, Rosebrough NJ, Farr AL Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193 : 265 275.
15. Miller JH (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
16. Neidhardt FC Umbarger HE (1996) Chemical composition of Escherichia coli. Escherichia coli and Salmonella: Cellular and Molecular Biology (Neidhardt FC et al., eds), pp. 13 16. ASM Press, Washington, DC.
17. Peakman T, Crouzet J, Mayaux J, Busby S, Mohan S, Harborne N, Wooton J, Nicolson R Cole J (1990) Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli chromosome. Eur J Biochem 191 : 315 323.
18. Rossmann R, Sawers G Böck A (1991) Mechanism of regulation of the formate-hydrogenlyase pathway by oxygen, nitrate, and pH: definition of the formate regulon. Mol Microbiol 5 : 2807 2814.
19. Sawers G (1994) The hydrogenases and formate dehydrogenases of Escherichia coli. Antonie van Leeuwenhoek 66 : 57 88.
20. Sawers G Böck A (1989) Novel transcriptional control of the pyruvate formate-lyase gene: upstream regulatory sequences and multiple promoters regulate anaerobic expression. J Bacteriol 171 : 2485 2498.
21. Sawers RG (2005a) Formate and its role in hydrogen production in Escherchia coli. Biochem Soc T 33 : 42 46.
22. Sawers RG (2005b) Evidence for novel processing of the anaerobically inducible dicistronic focA-pfl mRNA transcript in Escherichia coli. Mol Microbiol 58 : 1441 1453.
23. Sawers RG Clark DP (2004) Fermentative pyruvate and acetyl CoA metabolism. Chapter 3.5.3. EcoSal - Escherichia coli and Salmonella: Cellular and Molecular Biology (Curtiss R III., (Editor in Chief) ASM Press, Washington, DC. Available at.
24. Sawers RG, Blokesch M Böck A (2004) Anaerobic formate and hydrogen metabolism. Chapter 3.5.4. EcoSal - Escherichia coli and Salmonella: Cellular and Molecular Biology (Curtiss R III., (Editor in Chief) ASM Press, Washington, DC. Available at.
25. Schägger H von Jagow G (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal Biochem 199 : 223 231.
26. Schmidt TG Skerra A (2007) The Strp-tag system for one-step purification and high-affinity detection or capturing of proteins. Nat Protoc 2 : 1528 1535.
27. Simons RW, Houman F Kleckner N (1987) Improved single and multicopy lac-based protein and operon fusion cloning tools. Gene 53 : 85 96.
28. Suppmann B Sawers G (1994) Isolation and characterization of hypophosphite-resistant mutants of Escherichia coli: identification of the FocA protein, encoded by the pfl operon, as a putative formate transporter. Mol Microbiol 11 : 965 982.
29. Towbin H, Staehlin T Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide sheets: procedure and some applications. P Natl Acad Sci USA 76 : 4350 4354.
30. White WB Ferry JG (1992) Identification of formate dehydrogenase-specific mRNA species and nucleotide sequence of the fdhC gene of Methanobacterium formicicum. J Bacteriol 174 : 4997 5004.