Silencing specific paramecium tetraurelia genes by feeding double-stranded RNA

Cold Spring Harbor Protocols

Volumn 5, Issue 1, 2010, Pages

  Beisson, Janine ^a   Bétermier, Mireille ^a   Bré, Marie Hélène ^a   Cohen, Jean ^a   Duharcourt, Sandra ^b   Duret, Laurent ^c   Kung, Ching ^d   Malinsky, Sophie ^b   Meyer, Eric ^b   Preer Jr , John R ^e   Sperling, Linda ^a  

^a CNRS   (France)

^b ECOLE NORMALE SUPÉRIEURE   (France)

^c UNIVERSITÉ LYON 1   (France)

^d UNIVERSITY OF WISCONSIN MADISON   (United States)

^e INDIANA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 74349113093     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5363     Document Type: Article

References (7)

1. Beisson J, Bétermier M, Bré M-H, Cohen J, Duharcourt S, Duret L, Kung C, Malinsky S, Meyer E, Preer JR Jr, et al. 2010a. Maintaining clonal Paramecium tetraurelia cell lines of controlled age through daily reisolation. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5361.
2. Beisson J, Bétermier M, Bré M-H, Cohen J, Duharcourt S, Duret L, Kung C, Malinsky S, Meyer E, Preer JR Jr, et al. 2010b. Mass culture of Paramecium tetraurelia. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5362.
3. Beisson J, Bétermier M, Bré M-H, Cohen J, Duharcourt S, Duret L, Kung C, Malinsky S, Meyer E, Preer JR Jr, et al. 2010c. Paramecium tetraurelia: The renaissance of an early unicellular model. Cold Spring Harb Protoc (this issue). doi: 10.1101/ pdb.emo140.
4. Galvani A, Sperling L. 2002. RNA interference by feeding in Paramecium. Trends Genet 18: 11-12.
5. Kamath RS, Martinez-Campos M, Zipperlen P, Fraser AG, Ahringer J. 2001. Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol 2: doi: research0002.1-0002.10.
6. Timmons L, Fire A. 1998. Specific interference by ingested dsRNA. Nature 395: 854.
7. Timmons L, Court DL, Fire A. 2001. Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene 263: 103-112.