Biolistic transformation of Schistosoma mansoni: Studies with modified reporter-gene constructs containing regulatory regions of protease genes

Molecular and Biochemical Parasitology

Volumn 170, Issue 1, 2010, Pages 37-40

  Dvořák, Jan ^a,b   Beckmann, Svenja ^c   Lim, Kee Chong ^a   Engel, Juan C ^a   Grevelding, Christoph G ^c   McKerrow, James H ^a   Caffrey, Conor R ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b INSTITUTE OF PARASITOLOGY   (Czech Republic)

^c JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

Biolistics; Electroporation; Gene promoter; Protease; Schistosoma; Transgene

Indexed keywords

CATHEPSIN D; CATHEPSIN F;

ARTICLE; BIOLISTIC TRANSFORMATION; CONTROLLED STUDY; ELECTROPORATION; GENE CONSTRUCT; GENE EXPRESSION; GENOMIC FRAGMENT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN LOCALIZATION; REPORTER GENE; SCHISTOSOMA MANSONI; SIGNAL TRANSDUCTION; TRANSGENE;

ANIMALS; BIOLISTICS; CATHEPSIN D; CATHEPSIN F; GENE EXPRESSION REGULATION; GENES, REPORTER; HELMINTH PROTEINS; PEPTIDE HYDROLASES; REGULATORY SEQUENCES, NUCLEIC ACID; SCHISTOSOMA MANSONI; TRANSFORMATION, GENETIC;

PLATYHELMINTHES; SCHISTOSOMA; SCHISTOSOMA MANSONI;

EID: 74249121132     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2009.11.001     Document Type: Article

References (20)

1. Steinmann P., Keiser J., Bos R., Tanner M., and Utzinger J. Schistosomiasis and water resources development: systematic review, meta-analysis, and estimates of people at risk. Lancet Infect Dis 6 (2006) 411-425
2. Delcroix M., Sajid M., Caffrey C.R., et al. A multienzyme network functions in intestinal protein digestion by a platyhelminth parasite. J Biol Chem 281 (2006) 39316-39329
3. Caffrey C.R., McKerrow J.H., Salter J.P., and Sajid M. Blood 'n' guts: an update on schistosome digestive peptidases. Trends Parasitol 20 (2004) 241-248
4. Kines K.J., Morales M.E., Mann V.H., Gobert G.N., and Brindley P.J. Integration of reporter transgenes into Schistosoma mansoni chromosomes mediated by pseudotyped murine leukemia virus. FASEB J 22 (2008) 2936-2948
5. Morales M.E., Mann V.H., Kines K.J., et al. piggyBac transposon mediated transgenesis of the human blood fluke, Schistosoma mansoni. FASEB J 21 (2007) 3479-3489
6. Correnti J.M., and Pearce E.J. Transgene expression in Schistosoma mansoni: introduction of RNA into schistosomula by electroporation. Mol Biochem Parasitol 137 (2004) 75-79
7. Wippersteg V., Kapp K., Kunz W., Jackstadt W.P., Zahner H., and Grevelding C.G. HSP70-controlled GFP expression in transiently transformed schistosomes. Mol Biochem Parasitol 120 (2002) 141-150
8. Ndegwa D., Krautz-Peterson G., and Skelly P.J. Protocols for gene silencing in schistosomes. Exp Parasitol 117 (2007) 284-291
9. Grevelding C.G. Transgenic flatworms. In: Maule A.G., and Marks N.J. (Eds). Parasitic flatworms: molecular biology, biochemistry, immunology and physiology (2006), CAB International, Wallingford 149-173
10. Mann V.H., Morales M.E., Kines K.J., and Brindley P.J. Transgenesis of schistosomes: approaches employing mobile genetic elements. Parasitology 135 (2008) 141-153
11. Wippersteg V., Sajid M., Walshe D., et al. Biolistic transformation of Schistosoma mansoni with 5′ flanking regions of two peptidase genes promotes tissue-specific expression. Int J Parasitol 35 (2005) 583-589
12. Schulmeister A., Heyers O., Morales M.E., et al. Organization and functional analysis of the Schistosoma mansoni cathepsin D-like aspartic protease gene promoter. Biochim Biophys Acta 1727 (2005) 27-34
13. Newbury S.F. Control of mRNA stability in eukaryotes. Biochem Soc Trans 34 (2006) 30-34
14. Shaner N.C., Campbell R.E., Steinbach P.A., Giepmans B.N., Palmer A.E., and Tsien R.Y. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22 (2004) 1567-1572
15. Dresden M.H., Payne D.C., and Basch P.F. Acidic thiol proteinase activity of Schistosoma mansoni cultured in vitro. Mol Biochem Parasitol 6 (1982) 203-208
16. Basch P.F. Cultivation of Schistosoma mansoni in vitro I. Establishment of cultures from cercariae and development until pairing. J Parasitol 67 (1981) 179-185
17. Brindley P.J., Kalinna B.H., Wong J.Y., et al. Proteolysis of human hemoglobin by schistosome cathepsin D. Mol Biochem Parasitol 112 (2001) 103-112
18. Brady C.P., Dowd A.J., Brindley P.J., Ryan T., Day S.R., and Dalton J.P. Recombinant expression and localization of Schistosoma mansoni cathepsin L1 support its role in the degradation of host hemoglobin. Infect Immun 67 (1999) 368-374
19. Correnti J.M., Jung E., Freitas T.C., and Pearce E.J. Transfection of Schistosoma mansoni by electroporation and the description of a new promoter sequence for transgene expression. Int J Parasitol 37 (2007) 1107-1115
20. Yuan X.S., Shen J.L., Wang X.L., et al. Schistosoma japonicum: a method for transformation by electroporation. Exp Parasitol 111 (2005) 244-249