Adsorption induced enzyme denaturation: The role of polymer hydrophobicity in adsorption and denaturation of α-chymotrypsin on allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymers

Langmuir

Volumn 26, Issue 2, 2010, Pages 1096-1106

  Lahari, Challa ^a   Jasti, Lakshmi S ^a   Fadnavis, Nitin W ^a   Sontakke, Kalpana ^b   Ingavle, Ganesh ^b   Deokar, Sarika ^b   Ponrathnam, Surendra ^b  

^a INDIAN INSTITUTE OF CHEMICAL TECHNOLOGY   (India)

^b NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ADSORBED ENZYME; ALLYL GLYCIDYL ETHERS; AMBERLITE XAD-2; AQUEOUS BUFFER; AQUEOUS SOLUTIONS; CROSS-LINK DENSITIES; ENZYME ADSORPTION; ENZYME DENATURATION; ETHYLENE GLYCOL DIMETHACRYLATE; HYDROPHOBIC INTERACTIONS; LANGMUIR ADSORPTION ISOTHERMS; MACROPOROUS; MOLAR RATIO; POLAR INTERACTIONS; POLYMERIC SUPPORTS; RAMAN SPECTRA; ROOM TEMPERATURE; SECONDARY STRUCTURES;

ADSORPTION; COPOLYMERIZATION; DENATURATION; ENZYMES; ESTERS; ETHERS; ETHYLENE; ETHYLENE GLYCOL; FUNCTIONAL GROUPS; HYDROPHOBICITY; NAPHTHALENE; PLASTIC PRODUCTS; RAMAN SPECTROSCOPY;

COPOLYMERS;

ALLYL GLYCIDYL ETHER; ALPHA-CHYMOTRYPSIN; CHYMOTRYPSIN; CHYMOTRYPSIN A; EPOXIDE; ETHYLENE GLYCOL DIMETHACRYLATE; METHACRYLIC ACID DERIVATIVE; POLYMER;

ADSORPTION; ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; HYDROPHOBICITY; PROTEIN DENATURATION; RAMAN SPECTROMETRY; THEORETICAL MODEL;

ADSORPTION; CHYMOTRYPSIN; CIRCULAR DICHROISM; EPOXY COMPOUNDS; HYDROPHOBICITY; METHACRYLATES; MODELS, THEORETICAL; POLYMERS; PROTEIN DENATURATION; SPECTRUM ANALYSIS, RAMAN;

EID: 74249084261     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la904114u     Document Type: Article

References (67)

1. Horbett, T. A.; Brash, J. L. Proteins at Interfaces II, ACS Symposium Series 602; American Chemical Society: Washington DC, 1995.
2. Lienqueo, M. E.; Mahn, A.; Salgado, J. C.; Asenjo, J. A. J. Chromatogr. B 2007, 549, 53-68.
3. Weinberger, S. R.; Morris, T. S.; Pawlak, M. Pharmacogenomics 2000, 1, 395-416.
4. (a) Mosbach, R., Ed; Methods Enzymology; Academic Press: New York; Vols. 134-136.
5. (b) Bickerstaff, G. K., Ed; Immobilization of Enzymes and Cells; Humana Press: N.J., 1997.
6. (c) Guisan, J. M., Ed.; Immobilization of Enzymes and Cells, 2nd ed.; Humana Press: N.J., 2006.
7. (d) Mateo, C.; Palomo, J. M.; Fernandez-Lorente, G.; Guisan, J. M.; Fernandez-Lafuente, R. Enzyme Microb. Technol. 2007, 40, 1451-1463.
8. (e) Cao, L. Curr. Opin. Chem. Biol. 2005, 9, 217-226.
9. Katchalski-Katzir, E.; Kraemer, D. M. J. Mol. Catal. B: Enzymatic 2000, 10, 157-176.
10. (a) Bayramoǧlu, G.; Aríca, M. Y. Mol. Catal. B: Enzymatic 2008, 55, 76-83.
11. (b) Pujari, N. S.; Vaidya, B. K.; Bagalkote, S.; Ponrathnam, S.; Nene, S. J. Membr. Sci. 2006, 253, 395-403.
12. (c) Vaidya, B. K.; Ingavle, G. C.; Ponrathnam, S.; Kulkarni, B. D.; Nene, S. N. Bioresour. Technol. 2008, 99, 3623-3629.
13. (d) Miletić, N.; Vukovie, Z.; Nastasović, A.; Loos, R. J. Mol. Catal. B: Enzymatic 2009, 56, 196-201.
14. (a) Fernandez-Lorente, G.; Cabrera, Z.; Godoy, C.; Fernandez-Lafuente, R.; Palomo, J. M.; Guisan, J. M. Process Biochem. 2008, 43, 1061-1067.
15. (b) He, J.; Xu, Y.; Ma, H.; Zhang, Q.; Evans, D. G.; Duan, X. J. Colloid Interface Sci. 2006, 298, 780-786.
16. (c) Macario, A.; Katovic, A.; Giordano, G.; Fomi, L.; Carloni, F.; Filippini, A.; Setti, L. Stud. Surf. Sci. Catal. 2005, 155, 381-394.
17. (a) Al-Duri, B.; Yong, Y. P. Biochem, Eng. J. 2000, 4, 207-215.
18. (b) Bryjak, J.; Rolarz, B. N. Process Biochem. 1998, 33, 409-417.
19. (c) Zoungrana, T.; Findenegg, G. H.; Norde, W. J. Colloid Interface. Sci. 1997, 190, 437-448.
20. Bryjak, J.; Andrzej, W.; Trochimczuk, A. W. Chem. Eng. J. 1997, 65, 249-256.
21. (a) Liese, K.; Seelbach, D.; Wandrey, C. Industrial Biotransformations, 2nd ed.; Wiley-VCH: Weinheim, Germany, 2006.
22. (b) Faber, K. Biotransformations in Organic Chemiury, 5th ed.; Springer: Heidelberg, Germany, 2004.
23. Fadnavis, N. W.; Luisi, P. L. Biotechnol. Bioeng. 1989, 33, 1277-1282.
24. (a) Choi, S. H.; Kim, G. T.; Nho, Y. C. J. Appl. Polym. Sci. 1999, 71, 999-1006.
25. (b) Choi, S. H.; Lee, K. P.; Lee, J. G. Microchem. J. 2001, 05, 205-213.
26. (c) Lee, K. P.; Kang, H. J.; Joo, D. L.; Choi, S. H. Radiat. Phys. Chem. 2001, 60, 473-482.
27. (a) Choi, S. H.; Lee, R. P.; Kang, H. D. J. Appl. Polym. Sci. 2003, 88, 1153-1161.
28. (b) Malmsten, M.; Larsson, A. Colloids Surf. B 2000, 18, 277-284.
29. (c) Yasuda, M.; Kasahara, H.; Kawahara, K.; Ogino, H.; Ishikawa, H. Macromol. Chem. Phys. 2001, 202, 3189-3197.
30. (d) Chellapandian, M.; Krishnan, R. V. Process Biochem. 1998, 33, 595-600.
31. Material Safety Data Sheet, Merck.
32. International Chemical Safety Card ICSC 1270 (http://actrav.itcilo.org/ actrav-english/telearn/osh/ic/97905.htm).
33. Cornette, J.; Cease, R. B.; Margalit, H.; Spouge, J. L.; Berzofsky, J. A.; De Lisi, C. J. Mol. Biol. 1987, 195, 659-685.
34. (a) Jennissen, H. P.; Heilmeyer, L. M. G. Biochemistry 1975, 14, 754-759.
35. (b) Lin, F. Y.; Chen, W. Y.; Sang, L. C. Chromatgr. A 2000, 572, 37-47.
36. (c) Hofstee, B. H. J. Biochem. Biophys. Res. Commun. 1973, 53, 1137-1144.
37. (d.) Hjerten, S.; Rosengren, J.; Pahlman, S. J. Chromatogr. 1974, 101, 281-288.
38. Taylor, M.; Urquhart, A. J.; Zelzer, M.; Davies, M. C.; Picoliter, M. R. Langmuir 2007, 23, 6875-6878.
39. (a) Hansch, C.; Leo, A. Subsitution constants for correlation analysis in chemistry and biology; Wiley: New York, 1979.
40. (b) Hansch, C. Acc. Chem. Res. 1993, 26, 147-153.
41. Kong, X. Q.; Shea, D.; Gebreyes, W. A.; Xia, X. R. Anal. Chem. 2005, 77, 1275-1281.
42. Rosell, C. M.; Vaidya, A. M.; Hailing, P. J. Biochim. Biophys. Acta 1995, 1252, 158-164.
43. Hjerten, S.; Yao, K.; Eriksson, K. O.; Johansson, B. J. Chromatogr. 1986, 359, 99-109.
44. Engel, M. F. M.; van Mierlo, C. P. M.; Visser, A. J. W. G. J. Biol. Chem. 2002, 277, 10922-10930.
45. Chen, J.; Cramer, S. M. J. Chromatogr. A 2007, 1165, 67-77.
46. (a) Jennissen, H. P.; Heilmeyer, L. M. G. Biochemistry 1975, 14, 754-759.
47. (b) Lin, F. Y.; Chen, W. Y.; Sang, L. C. J. Chromatgr. A 2000, 872, 37-47.
48. (c) Hofstee, B. H. J. Biochem. Biophys. Res. Commun. 1973, 53, 1137-1144.
49. (d) Hjerten, S.; Rosengren, J.; Pahlman, S. J. Chromatogr. 1974, 101, 281-288.
50. Lencki, R. W.; Arul, J.; Neufeld, R. Biotechnol. Bioeng. 1992, 40, 1421-1426.
51. (a) Morriset, J. D.; Broomfield, C. A. J. Am. Chem. Soc. 1971, 93, 7297-7304.
52. (b) Hennessey, J. P.; Johnson, W. C., Jr. Biochemistry 1981, 20, 1085-1094.
53. (c) Sasaki, T.; Kobayashi, M.; Kise, H. Biotechnol. Techniq. 1997, 11, 387-390.
54. Rezaei-Ghaleh, N.; Ramshini, H.; Ebrahim-Habibi, A.; Moosavi-Movahedi, A. A.; Nemat-Gorgani, M. Biophys. Chem. 2008, 132, 23-32.
55. Sackett, D. L.; Wolff, J. Anal. Biochem. 1987, 167, 228-234.
56. Sutter, M.; Oliviera, S.; Sanders, N. R.; Lukas, B.; van Hoek, A.; Hink, M. A.; Visser, A. J. W. G.; de Smedt, S. C.; Hennink, W. E.; Jiskoot, W. J. Fluorsc. 2007, 17, 181-192.
57. (a) Heremans, L.; Heremans, K. Biochim. Biophys. Acta 1989, 999, 192-197.
58. (b) Lord, R. C.; Yu, N. T. Mol. Biol. 1970, 51, 203-213.
59. Sigma-Aldrich Product Information Catalog, www.sigma-aldrich.com
60. (a) Dill, K. A. Biochemistry 1990, 29, 7133-7155.
61. (b) Pace, C. N.; Shirley, B. A.; Mcnutt, M.; Gajiwala, K. FASEB 1996, 10, 75-83.
62. (c) Makhatadze, G. I.; Privalov, P. L. Biophys. Chem. 1994, 51, 291-309.
63. (d) Cooper, A. Biophys. Chem. 2000, 53, 25-39.
64. (e) Otto, S.; Engberts, J. B. F. N. Org. Biomol. Chem. 2003, 7, 2809-2820.
65. (f) Jorgensen, W. L.; Gao, J.; Ravimohan, C. J. Phys. Chem. 1985, 89, 3470-3473.
66. Ben Nairn, A. Biophys. Chem. 2003, 103, 183-193.
67. Fadnavis, N. W.; Seshadri, R.; Sheelu, G.; Vasantha Madhuri, K. Arch. Biochem. Biophys. 2005, 433, 454-465.