Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry

Environmental Pollution

Volumn 158, Issue 3, 2010, Pages 948-952

  Neffling, Milla Riina ^a   Lance, Emilie ^b   Meriluoto, Jussi ^a  

^a ÅBO AKADEMI UNIVERSITY   (Finland)

^b UNIVERSITÉ DE RENNES 1   (France)

Author keywords

Microcystins; MMPB method; Total contamination levels

Indexed keywords

4-PHENYLBUTYRIC ACID; ANIMAL TISSUE; CONTAMINATION LEVELS; COVALENT INTERACTIONS; COVALENTLY BOUND; EXTRACTION EFFICIENCIES; HEPATOTOXINS; LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY; MATRIX; METHOD DEVELOPMENT; METHOXY; MICROCYSTINS; PROTEIN PHOSPHATASE; SIGNAL SUPPRESSION;

ANIMALS; CHROMATOGRAPHIC ANALYSIS; HISTOLOGY; LIQUID CHROMATOGRAPHY; MANAGEMENT; MASS SPECTROMETRY; ORGANIC ACIDS; PROTEINS; TOXIC MATERIALS;

EXTRACTION;

2 METHYL 3 METHOXY 4 PHENYLBUTYRIC ACID; ARYLBUTYRIC ACID DERIVATIVE; MICROCYSTIN; UNCLASSIFIED DRUG;

BIOACCUMULATION; CONCENTRATION (COMPOSITION); CYANOBACTERIUM; EFFICIENCY MEASUREMENT; INHIBITION; LABORATORY METHOD; OPTIMIZATION; PHOSPHATASE; PROTEIN; TOXIN;

ANIMAL TISSUE; ARTICLE; BIOACCUMULATION; BLOOD ANALYSIS; CONTAMINATED ORGANISM; CONTROLLED STUDY; COVALENT BOND; COW; EXTRACTION; LIQUID CHROMATOGRAPHY; NONHUMAN; OXIDATION; PROTEIN BINDING; SIGNAL PROCESSING; SNAIL; TANDEM MASS SPECTROMETRY;

ANIMALS; BACTERIAL TOXINS; CHROMATOGRAPHY, LIQUID; CYANOBACTERIA; MICROCYSTINS; MOLECULAR STRUCTURE; PROTEIN BINDING; SNAILS; TANDEM MASS SPECTROMETRY;

ANIMALIA; CYANOBACTERIA;

EID: 74149091840     PISSN: 02697491     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.envpol.2009.10.023     Document Type: Article

References (24)

1. van Apeldoorn M.E., van Egmond H.P., Speijers G.J., and Bakker G.J. Toxins of cyanobacteria. Mol. Nutr. Food Res. 51 (2007) 7-60
2. Codd G.A., Morrison L.F., and Metcalf J.S. Cyanobacterial toxins: risk management for health protection. Toxicol. Appl. Pharmacol. 203 (2005) 264-272
3. Harada K., Murata H., Qiang Z., Suzuki M., and Kondo F. Mass spectrometric screening method for microcystins in cyanobacteria. Toxicon 34 (1996) 701-710
4. Hastie C.J., Borthwick E.B., Morrison L.F., Codd G.A., and Cohen P.T.W. Inhibition of several protein phosphatases by a non-covalently interacting microcystin and a novel cyanobacterial peptide, nostocyclin. Biochim. Biophys. Acta 1726 (2005) 187-193
5. Honkanen R.E., Zwiller J., Moore R.E., Daily S.L., Khatra B.S., Dukelow M., and Boynton A.L. Characterization of microcystin-LR, a potent inhibitor of type 1 and type 2A protein phosphatases. J. Biol. Chem. 265 (1990) 19401-19404
6. Imanishi S., and Harada K. Proteomics approach on microcystin binding proteins in mouse liver for investigation of microcystin toxicity. Toxicon 43 (2004) 651-659
7. Karlsson K., Sipia V., Kankaanpaa H., and Meriluoto J. Mass spectrometric detection of nodularin and desmethylnodularin in mussels and flounders. J. Chromatogr. B 784 (2003) 243-253
8. Kaya K., and Sano T. Total microcystin determination using erythro-2-methyl-3-(methoxy-d(3))-4-phenylbutyric acid (MMPB-d(3)) as the internal standard. Anal. Chim. Acta 386 (1999) 107-112
9. Kondo F., Ikai Y., Oka H., Okumura M., Ishikawa N., Harada K., Matsuura K., Murata H., and Suzuki M. Formation, characterization, and toxicity of the glutathione and cysteine conjugates of toxic heptapeptide microcystins. Chem. Res. Toxicol. 5 (1992) 591-596
10. Lance, E., Neffling, M.-R, Gérard, C., Meriluoto, J., Bormans, M., Accumulation of free and covalently bound microcystins in tissues of Lymnaea stagnalis (Gastropoda) following toxic cyanobacteria or dissolved microcystin-LR exposure. Environ. Pollut., in press, doi:10.1016/j.envpol.2009.10.025.
11. MacKintosh C., Beattie K.A., Klumpp S., Cohen P., and Codd G.A. Cyanobacterial microcystin-LR is a potent and specific inhibitor of protein phosphatases 1 and 2A from both mammals and higher plants. FEBS Lett. 264 (1990) 187-192
12. Mikhailov A., Härmälä-Braskén A.S., Hellman J., Meriluoto J., and Eriksson J.E. Identification of ATP-synthase as a novel intracellular target for microcystin-LR. Chem. Biol. Interact 142 (2003) 223-237
13. Ott J.L., and Carmichael W.W. LC/ESI/MS method development for the analysis of hepatotoxic cyclic peptide microcystins in animal tissues. Toxicon 47 (2006) 734-741
14. Pelander A., Backström D., and Ojanperä I. Qualitative screening for basic drugs in autopsy liver samples by dual-plate overpressured layer chromatography. J. Chromatogr. B Analyt Technol. Biomed. Life Sci. 857 (2007) 337-340
15. Rinehart K.L., Harada K., Namikoshi M., Chen C., Harvis C.A., Munro M.H.G., Blunt J.W., Mulligan P.E., Beasley V.R., Dahlem A.M., and Carmichael W.W. Nodularin, microcystin, and the configuration of Adda. J. Am. Chem. Soc. 110 (1988) 8557-8558
16. Robinson N.A., Matson C.F., and Pace J.G. Association of microcystin-LR and its biotransformation product with a hepatic-cytosolic protein. J. Biochem. Toxicol. 6 (1991) 171-180
17. Sano T., Nohara K., Shiraishi F., and Kaya K. A method for microdetermination of total microcystin content in waterblooms of cyanobacteria (Blue-Green-Algae). Int. J. Environ. Ch 49 (1992) 163-170
18. Tsuji K., Masui H., Uemura H., Mori Y., and Harada K. Analysis of microcystins in sediments using MMPB method. Toxicon 39 (2001) 687-692
19. WHO. Guidelines for Drinking-water Quality (1998), World Health Organization, Geneva
20. Williams D.E., Craig M., McCready T.L., Dawe S.C., Kent M.L., Holmes C.F., and Andersen R.J. Evidence for a covalently bound form of microcystin-LR in salmon liver and dungeness crab larvae. Chem. Res. Toxicol. 10 (1997) 463-469
21. Williams D.E., Dawe S.C., Kent M.L., Andersen R.J., Craig M., and Holmes C.F. Bioaccumulation and clearance of microcystins from salt water mussels, Mytilus edulis, and in vivo evidence for covalently bound microcystins in mussel tissues. Toxicon 35 (1997) 1617-1625
22. Williams D.E., Craig M., Dawe S.C., Kent M.L., Andersen R.J., and Holmes C.F. 14C-labeled microcystin-LR administered to Atlantic salmon via intraperitoneal injection provides in vivo evidence for covalent binding of microcystin-LR in salmon livers. Toxicon 35 (1997) 985-989
23. Young F.M., Metcalf J.S., Meriluoto J.A.O., Spoof L., Morrison L.F., and Codd G.A. Production of antibodies against microcystin-RR for the assessment of purified microcystins and cyanobacterial environmental samples. TOXICON 48 (2006) 295-306
24. Yuan M., Carmichael W.W., and Hilborn E.D. Microcystin analysis in human sera and liver from human fatalities in Caruaru, Brazil 1996. Toxicon 48 (2006) 627-640