Characterization of a new Cu/Zn-superoxide dismutase from Beauveria bassiana and two site-directed mutations crucial to its antioxidation activity without chaperon

Enzyme and Microbial Technology

Volumn 46, Issue 3-4, 2010, Pages 217-222

  Xie, Xue Qin ^a   Ying, Sheng Hua ^a   Feng, Ming Guang ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Antioxidation activity enhancement; Beauveria bassiana; Copper chaperon; Site directed mutation; Superoxide dismutase

Indexed keywords

ANTIOXIDATION ACTIVITIES; BEAUVERIA BASSIANA; CRUDE EXTRACT; CU/ZN-SOD; CU/ZN-SUPEROXIDE DISMUTASE; E. COLI; FUNCTION ANALYSIS; GENE CLONING; HETEROGENEOUS EXPRESSIONS; LURIA-BERTANI MEDIUMS; SEQUENCE IDENTITY; SITE-DIRECTED MUTATION; SUPER OXIDE DISMUTASE;

CELL CULTURE; CLONING; COPPER; ESCHERICHIA COLI; OXYGEN; ZINC;

METAL REFINING;

CHAPERONE; COPPER ZINC SUPEROXIDE DISMUTASE; GENOMIC DNA; ISOPROPYL THIOGALACTOSIDE; LYSINE; RNA;

ANTIOXIDANT ACTIVITY; ARTICLE; BEAUVERIA; DNA EXTRACTION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ENGINEERING; ENZYME PURIFICATION; ESCHERICHIA COLI; FUNGAL STRAIN; FUNGUS GROWTH; FUNGUS MUTATION; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; RNA EXTRACTION; SEQUENCE ANALYSIS;

CORDYCEPS BASSIANA; ESCHERICHIA COLI; FUNGI; LURIA;

EID: 74149090314     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2009.09.005     Document Type: Article

References (38)

1. Fridovich I. Superoxide radical: an endogenous toxicant. Annu Rev Pharmacol Toxicol 23 (1983) 239-257
2. Fridovich I. Superoxide radical and superoxide dismutases. Annu Rev Biochem 64 (1995) 97-112
3. Yue W.F., Yao M.L., Liu J.M., Li G.L., Li X.H., Wu X.F., et al. Manganese superoxide dismutase expressed in silkworm larvae, Bombyx mori L enhances the NK activity and splenocyte proliferation against Sarcoma 180 tumor cells in vivo. Mol Biol Rep 36 (2009) 187-192
4. Gao F., Koenitzer J.R., Tobolewski J.M., Jiang D.H., Liang J.R., Noble P.W., et al. Extracellular superoxide dismutase inhibits inflammation by preventing oxidative fragmentation of hyaluronan. J Biol Chem 283 (2008) 6058-6066
5. van Deel E.D., Lu Z.B., Xu X., Zhu G.S., Hu X.L., Oury T.D., et al. Extracellular superoxide dismutase protects the heart against oxidative stress and hypertrophy after myocardial infarction. Free Rad Biol Med 44 (2008) 1305-1313
6. Ishihara T., Tanaka K.I., Tasaka Y., Namba T., Suzuki J., Ishihara T., et al. Therapeutic effect of lecithinized superoxide dismutase against colitis. J Pharmacol Exp Ther 328 (2009) 152-164
7. Kumar S, Sahoo R, Ahuja PS. Novel purified isozyme of autoclavable superoxide dismutase extracted from the plant Potentilla atrosanguinea Lodd. var. argyrophylla, useful in cosmetic, pharmaceutical and food compositions. US Patent 6485950-B1 (2003).
8. Bhardwaj PK, Sahoo R, Kumar S, Ahuja PS, Bhardwaj P, Ahuja P. New superoxide dismutase (SOD) obtained from Potentilla atrosanguinea, useful in pharmaceuticals and medical fields, and in cosmetic and food industry. World Patent 2007113615-A1 (2007).
9. Casareno R.L.B., Waggoner D., and Gitlin J.D. The copper chaperone CCS directly interacts with copper/zinc superoxide dismutase. J Biol Chem 273 (1998) 23625-23628
10. Schmidt P.J., Kunst C., and Culotta V.C. Copper activation of superoxide dismutase 1 (SOD1) in vivo: role for protein-protein interactions with the copper chaperone for SOD1. J Biol Chem 275 (2000) 33771-33776
11. Furukawa Y., Torres A.S., and Halloran T.V. Oxygen induced maturation of SOD1: a key role for disulfide formation by the copper chaperone CCS. EMBO J 23 (2004) 2872-2881
12. Horecka J., Kinsey P.T., and Sprague G.F. Cloning and characterization of the Saccharomyces cerevisiae lys7 gene - evidence for function outside of lysine biosynthesis. Gene 162 (1995) 87-92
13. Culotta V.C., Klomp L.W.J., Strain J., Casareno R.L.B., Krems B., and Gitlin J.D. The copper chaperone for superoxide dismutase. J Biol Chem 272 (1997) 23469-23472
14. Wintz H., and Vulpe C. Plant copper chaperones. Biochem Soc Trans 30 (2002) 732-735
15. Hiromura M., Chino H., Sonoda T., and Sakurai H. Molecular cloning and characterization of a copper chaperone for copper/zinc superoxide dismutase from the rat. Biochem Biophys Res Commun 275 (2000) 394-400
16. Moore S.D., Chen M.M., and Cox D.W. Cloning and mapping of murine CCS and mapping of the human orthology. Cytogenet Cell Genet 88 (2000) 35-37
17. Carroll M.C., Girouard J.B., Ulloa J.L., Subramaniam J.R., Wong P.C., Valentine J.S., et al. Mechanisms for activating Cu and Zn-containing superoxide dismutase in the absence of the CCS Cu chaperone. Proc Natl Acad Sci USA 101 (2004) 5964-5969
18. Beckman J.S., Estvez A.G., Barbeito L., and Crow J.P. CCS knockout mice establish an alternative source for copper for SOD in ALS. Free Rad Biol Med 33 (2002) 1433-1435
19. Jensen L.T., and Culotta V.C. Activation of CuZn superoxide dismutases from Caenorhabditis elegans does not require the copper chaperone CCS. J Biol Chem 280 (2005) 41373-41379
20. De Faria M.R., and Wraight S.P. Mycoinsecticides and mycoacaricides: a comprehensive list with worldwide coverage and international classification of formulation types. Biol Control 43 (2007) 237-256
21. Huang B.F., and Feng M.G. Comparative tolerances of various Beauveria bassiana isolates to UV-B irradiation with a description of a modeling method to assess lethal dose. Mycopathol 168 (2009) 145-215
22. Griffiths H.R., Mistry P., Herbert K.E., and Lunec J. Molecular and cellular effects of ultraviolet light-induced genotoxicity. Crit Rev Clin Lab Sci 35 (1998) 189-237
23. Reader U., and Broda P. Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1 (1985) 17-20
24. Kumar S., Nei M., Dudley J., and Tamura K. MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9 (2008) 299-306
25. Marklund S., and Marklund G. Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47 (1974) 248-254
26. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 (1976) 248-254
27. Mount S.M. A catalogue of splice junction sequences. Nucleic Acids Res 10 (1982) 459-472
28. Bordo D., Djinovic K., and Bolognesi M. Conserved patterns in the Cu,Zn superoxide dismutase family. J Mol Biol 238 (1994) 366-386
29. Borders C.J., Saunders J.E., Blech D.M., and Fridovich I. Essentially of the active site arginine residue for the normal catalytic activity of Cu,Zn superoxide dismutase. Biochem J 230 (1985) 771-776
30. Wong P.C., Waggoner D., Subramaniam J.R., Tessarollo L., Bartnikas T.B., Culotta V.C., et al. Copper chaperone for superoxide dismutase is essential to activate mammalian Cu/Zn superoxide dismutase. Proc Natl Acad Sci USA 97 (2000) 2886-2891
31. Angelova M., Dolashka-Angelova P., Ivanova E., Serkedjieva J., Slokoska L., Pashova S., et al. A novel glycosylated Cu/Zn-containing superoxide dismutase: production and potential therapeutic effect. Microbiol-SGM 147 (2001) 1641-1650
32. Seo H.S., Koo Y.J., Lim J.Y., Song J.T., Kim C.H., Kim J.K., et al. Characterization of a bifunctional enzyme fusion of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Escherichia coli. Appl Environ Microbiol 66 (2000) 2484-2490
33. Volkel T., Korn T., Bach M., Muller R., and Kontermann R.E. Optimized linker sequences for the expression of monomeric and dimeric bispecific single-chain diabodies. Protein Eng 14 (2001) 815-823
34. Angelova M., Genova L., Slokoska L., and Pashova S. Effect of glucose on the superoxide dismutase production in fungal strain Humicola lutea. Can J Microbiol 41 (1995) 978-983
35. Angelova M., Genova L., Pashova S., Slokoska L., and Dolashka P. Effect of cultural conditions on the synthesis of superoxide dismutase by Humicola lutea 110. J Ferment Bioeng 82 (1996) 464-468
36. Krumova E., Dolashka-Angelova P., Pashova S., Stefanova L., Van Beeumen J., Vassilev S., et al. Improved production by fed-batch cultivation and some properties of Cu/Zn-superoxide dismutase from the fungal strain Humicola lutea 103. Enzyme Micro Technol 40 (2007) 524-532
37. Wang Z.S., Gu Y.X., and Yuan Q.S. Effect of nutrition factors on the synthesis of superoxide dismutase, catalase, and membrane lipid peroxide levels in Cordyceps militaris mycelium. Curr Microbiol 52 (2006) 74-79
38. Raimondi S., Uccelletti D., Matteuzzi D., Pagnoni U.M., Rossi M., and Palleschi C. Characterization of the superoxide dismutase SOD1 gene of Kluyveromyces marxianus L3 and improved production of SOD activity. Appl Microbiol Biotechnol 77 (2008) 1269-1277