Purification and partial characterization of a 36-kDa chitinase from Bacillus thuringiensis subsp. colmeri, and its biocontrol potential

Enzyme and Microbial Technology

Volumn 46, Issue 3-4, 2010, Pages 252-256

  Liu, Dong ^a   Cai, Jun ^a,b,c   Xie, Chi chu ^a   Liu, Chuan ^a   Chen, Yue hua ^a,b,c  

^a NANKAI UNIVERSITY   (China)

^b BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^c Tianjin Key Laboratory of Microbial Functional Genomics   (China)

Author keywords

Antagonism; Bacillus thuringiensis; Characterization; ChiA; Dimmer; Synergism

Indexed keywords

ANTAGONISM; BACILLUS THURINGIENSIS; CHITINASES; CRYSTAL PROTEINS; GLYCOL CHITIN; HELICOVERPA ARMIGERA; INHIBITORY CONCENTRATION; LETHAL CONCENTRATION; OPTIMAL ACTIVITY; OPTIMUM PH; PARTIAL CHARACTERIZATION; PROTEIN BANDS; RENATURATION; SDS-PAGE; SEPHADEX; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; SPODOPTERA EXIGUA; SPORE GERMINATION; SURFACE CONTAMINATIONS;

BACTERIOLOGY; BIOASSAY; BIOCONTROL; DIFFUSERS (OPTICAL); ELECTROPHORESIS; ENZYMES; ESCHERICHIA COLI; GLYCOLS; ION EXCHANGE; MOLECULAR MASS; PURIFICATION; SILVER; SODIUM; SODIUM SULFATE; ZINC;

ENZYME ACTIVITY;

CHITIN; CHITINASE; GLYCOL; SEPHADEX; SILVER; ZINC ION;

ARTHROPOD LARVA; ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL STRAIN; BIOASSAY; BIOLOGICAL PEST CONTROL; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBSTRATE; ESCHERICHIA COLI; FUNGUS; FUNGUS SPORE; HELICOVERPA ARMIGERA; IC 50; LC 50; LEPIDOPTERA; MOLECULAR WEIGHT; NONHUMAN; PENICILLIUM; PENICILLIUM GLAUCUM; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN RENATURATION; SCLEROTINIA FUCKELIAN; SPODOPTERA EXIGUA; SPOROGENESIS; SUBSPECIES; TEMPERATURE;

BACILLUS THURINGIENSIS; BACILLUS THURINGIENSIS SEROVAR COLMERI; ESCHERICHIA COLI; FUNGI; HELICOVERPA ARMIGERA; PENICILLIUM; SCLEROTINIA; SPODOPTERA EXIGUA;

EID: 74049139078     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2009.10.007     Document Type: Article

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