Expression, purification and antibody preparation of flagellin FlaA from Vibrio alginolyticus strain HY9901

Letters in Applied Microbiology

Volumn 50, Issue 2, 2010, Pages 181-186

  Liang, H Y ^a,b,c,d,e   Xia, L Q ^c   Wu, Z H ^c,d,e   Jian, J C ^c,d,e   Lu, Y S ^c,d,e  

^a SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c GUANGDONG OCEAN UNIVERSITY   (China)

^d Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals and Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes   (China)

^e Key Lab of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Insts   (China)

Author keywords

Antibody preparation; Expression; Flagellin flaA gene; Immunogold electron microscopy; Purification; Vibrio alginolyticus

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; ANTIBODIES; CHROMATOGRAPHIC ANALYSIS; CLONING; ELECTRON MICROSCOPY; ESCHERICHIA COLI; ORGANIC ACIDS;

ALIGNMENT ANALYSIS; AMINO ACID RESIDUES; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION; FLAGELLIN FLAA GENE; FUSION PROTEINS; IMMUNOLOGICAL METHODS; OPEN READING FRAME; POLYCLONAL ANTIBODY; PUTATIVE PROTEINS; SDS-PAGE; STRUCTURAL STUDIES; VIBRIO ALGINOLYTICUS; VIBRIO SPECIES; WESTERN BLOTTING; WESTERN-BLOT ANALYSIS;

PURIFICATION;

ANTISERUM; FLAA ANTIBODY; FLAA PROTEIN; FLAGELLIN; HYBRID PROTEIN; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANTIBODY; BACTERIUM; CHROMATOGRAPHY; CLONE; ELECTRON MICROSCOPY; GENE EXPRESSION; IMMUNOASSAY; PATHOGEN; PROTEIN; VACCINE;

AFFINITY CHROMATOGRAPHY; ANTIBODY PRODUCTION; ANTIBODY TITER; ANTIGEN ANTIBODY REACTION; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE EXPRESSION; GENE IDENTIFICATION; IMMUNOGOLD ELECTRON MICROSCOPY; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE ALIGNMENT; VIBRIO ALGINOLYTICUS; WESTERN BLOTTING;

ESCHERICHIA COLI; VIBRIO; VIBRIO ALGINOLYTICUS;

EID: 74049101584     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2009.02768.x     Document Type: Article

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