Synergistic effects of iron and temperature on Antarctic phytoplankton and microzooplankton assemblages

Biogeosciences

Volumn 6, Issue 12, 2009, Pages 3131-3147

  Rose, J M ^a   Feng, Y ^b   Ditullio, G R ^c   Dunbar, R B ^d   Hare, C E ^e   Lee, P A ^c   Lohan, M ^f   Long, M ^d   Smith Jr , W O ^g   Sohst, B ^h   Tozzi, S ^g   Zhang, Y ^e   Hutchins, D A ^b  

^a WOODS HOLE OCEANOGRAPHIC INSTITUTION   (United States)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^c School of Business   (United States)

^d STANFORD UNIVERSITY   (United States)

^e UNIVERSITY OF DELAWARE   (United States)

^f UNIVERSITY OF PLYMOUTH   (United Kingdom)

^g VIRGINIA INSTITUTE OF MARINE SCIENCE   (United States)

^h Old Dominion University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABUNDANCE; ALGAL BLOOM; ANTAGONISM; CLIMATE CHANGE; IRON; MICROBIAL COMMUNITY; MULTIVARIATE ANALYSIS; NUTRIENT AVAILABILITY; PHOTOSYNTHESIS; PHYTOPLANKTON; POLAR REGION; SYNERGISM; TEMPERATURE EFFECT; ZOOPLANKTON;

ROSS SEA; SOUTHERN OCEAN;

ALGAE;

EID: 74049095072     PISSN: 17264170     EISSN: 17264189     Source Type: Journal    
DOI: 10.5194/bg-6-3131-2009     Document Type: Article

References (96)

1. Allen, A. E., LaRoche, J., Maheswari, U., Lommer, M., Schauer, N., Lopez, P. J., Finazzi, G., Fernie, A. R., and Bowler, C.: Whole-cell response of the pennate diatom Phaeodactylum tricornutum to iron starvation, Proc Natl Acad Sci USA, 105, 10438-10443, 2008.
2. Alley, R., Berntsen, T., Bindoff, N. L., Chen, Z., Chidthaisong, A., Friedlingstein, P., Gregory, J., Hegerl, G., Heimann, M., Hewitson, B., Hoskins, B., Joos, F., Jouzel, J., Kattsov, V., Lohmann, U., Manning, M., Matsuno, T., Molina, M., Nicholls, N., Overpeck, J., Qin, D., Raga, G., Ramaswamy, V., Ren, J., Rusticucci, M., Solomon, S., Somerville, R., Stocker, T. F., Stott, P., Stouffer, R. J., Whetton, P., Wood, R. A., and Wratt, D.: Climate change 2007: The physical science basis. Summary for policymakers, Intergovernmental Panel on Climate Change, Geneva, 2007.
3. Arrigo, K. R., Robinson, D. H., Worthen, D. L., Dunbar, R. B., DiTullio, G. R., VanWoert, M., and Lizotte, M. P.: Phytoplankton community structure and the drawdown of nutrients and CO2 in the Southern Ocean, Science, 283, 365-367, 1999.
4. Arrigo, K. R., Ditullio, G. R., Dunbar, R. B., Robinson, D. H., Van-Woert, M., Worthen, D. L., and Lizotte, M. P.: Phytoplankton taxonomic variability in nutrient utilization and primary production in the Ross Sea, J. Geophys Res., 105, 8827-8846, 2000.
5. Bertrand, E. M., Saito, M. A., Rose, J. M., Riesselman, C. R., Lohan, M. C., Noble, A. E., Lee, P. A., and DiTullio, G. R.: Vitamin B12 and iron colimitation of phytoplankton growth in the Ross Sea, Limnol. Oceanogr., 52, 1079-1093, 2007.
6. Blank, G. S., Robinson, D. H., and Sullivan, C. W.: Diatom mineralization of silicic acid VIII. metabolic requirements and the timing of protein synthesis, J. Phycol., 22, 382-389, 1986.
7. Boyd, P. W., LaRoche, J., Gall, M., Frew, R. D., and McKay, R.: Role of iron, light and silicate in controlling algal biomass in subantarctic waters SE of New Zealand, J. Geophys Res., 104, 13391-13404, 1999.
8. Boyd, P. W., Watson, A. J., Law, C. S., Abraham, E. R., Trull, T., Murdoch, R., Bakker, D. C. E., Bowie, A. R., Buesseler, K. O., Chang, H., Charette, M., Croot, P., Downing, K., Frew, R., Gall, M., Hadfield, M., Hall, J., Harvey, M., Jameson, G., LaRoche, J., Liddicoat, M., Ling, R., Maldonado, M. T., McDay, R. M., Nodder, S., Pickmere, S., Pridmore, R., Rintoul, S., Safi, K., Sutton, P., Strzepek, R., Tanneberger, K., Turner, S., Waite, A., and Zeldis, J.: A mesoscale phytoplankton bloom in the polar Southern Ocean stimulated by iron fertilization, Nature, 407, 695-702, 2000.
9. Boyd, P.W., Crossley, C., DiTullio, G. R., Griffiths, F. B., Hutchins, D. A., Queguiner, B., Sedwick, P. N., and Trull, T.W.: Control of phytoplankton growth by iron supply and irradiance in the subantarctic Southern Ocean: experimental results from the SAZ project, J. Geophys Res., 106, 31573-31583, 2001.
10. Boyd, P. W. and Doney, S. C.: Modelling regional responses by marine pelagic ecosystems to global climate change, Geophys. Res. Lett., 29, 1806, doi:10.1029/2001GL014130, 2002.
11. Bruland, K. W., Rue, E. L., Smith, G. J., and DiTullio, G. R.: Iron, macronutrients and diatom blooms in the Peru upwelling regime: brown and blue waters of Peru, Mar. Chem., 93, 81-103, 2005.
12. Brzezinski, M. A. and Nelson, D. M.: The annual silica cycle in the Sargasso Sea near Bermuda, Deep-Sea Res. I, 42, 1215-1237, 1995.
13. Bucciarelli, E., Pondaven, P., and Sarthou, G.: Effects of an ironlight co-limitation on the elemental composition (Si, C, N) of the marine diatoms Thalassiosira oceanica and Ditylum brightwellii, Biogeosciences Discuss., 6, 7175-7205, 2009, http://www.biogeosciences-discuss.net/6/7175/2009/.
14. Buck, K. N., Lohan, M. C., Berger, C. J. M., and Bruland, K. W.: Dissolved iron speciation in two distinct river plumes and an estuary: Implications for riverine iron supply, Limnol. Oceanogr., 52, 843-855, 2007.
15. Campbell, L.: Flow cytometric analysis of autotrophic picoplankton, in: Methods in Microbiology, Academic Press, 317-341, 2001.
16. Caron, D. A., Dennett, M. R., Lonsdale, D. J., Moran, D. M., and Shalapyonok, L.: Microzooplankton herbivory in the Ross Sea, Antarctica, Deep-Sea Res. II, 47, 3249-3272, 2000.
17. Clarke, K. R. and Green, R. H.: Statistical design and analysis for a 'biological effects' study, Mar. Ecol-Prog. Ser., 46, 213-226, 1988.
18. Clarke, K. R.: Non-parametric multivariate analyses of changes in community structure, Aust. J. Ecol., 18, 117-143, 1993.
19. Clarke, K. R. andWarwick, R. M.: Change in marine communities: an approach to statistical analysis and interpretation, 2nd edition, Plymouth Marine Laboratory, Plymouth, UK, 2001.
20. Clarke, K. R. and Gorley, R. N.: Primer v6: user manual/tutorial, Primer-E Ltd, Plymouth, 190 pp., 2006.
21. Coale, K. H., Wang, X., Tanner, S. J., and Johnson, K. S.: Phytoplankton growth and biological response to iron and zinc addition in the Ross Sea and Antarctic Circumpolar Current along 170°W, Deep-Sea Res. II, 50, 635-653, 2003.
22. Coale, K. H., Johnson, K. S., Chavez, F. P., Buesseler, K. O., Barber, R. T., Brzezinski, M. A., Cochlan, W. P., Millero, F. J., Falkowski, P. G., Bauer, J. E., Wanninkhof, R. H., Kudela, R. M., Altabet, M. A., Hales, B. E., Takahashi, T., Landry, M. R., Bidigare, R. R., Wang, X., Chase, Z., Strutton, P. G., Friederich, G. E., Gorbunov, M. Y., Lance, V. P., Hilting, A. K., Hiscock, M. R., Demarest, M., Hiscock, W. T., Sullivan, K. F., Tanner, S. J., Gordon, R. M., Hunter, C. N., Elrod, V. A., Fitzwater, S. E., Jones, J. L., Tozzi, S., Koblizek, M., Roberts, A. E., Herndon, J., Brewster, J., Ladizinsky, N., Smith, G., Cooper, D., Timothy, D., Brown, S. L., Selph, K. E., Sheridan, C. C., Twining, B. S., and Johnson, Z. I.: Southern Ocean iron enrichment experiment: carbon cycling in high- and low-Si waters, Science, 304, 408-414, 2004.
23. De Baar, H. J. W., Buma, A. G. J., Nolting, R. F., Cadee, G. C., Jacques, G., and Treguer, P. J.: On iron limitation of the Southern Ocean: experimental observations in the Weddell and Scotia Seas, Mar. Ecol-Prog. Ser., 65, 105-122, 1990.
24. del Valle, D. A., Kieber, D. J., Toole, D. A., Bisgrove, J., and Kiene, R. P.: Dissolved DMSO production via biological and photochemical oxidation of dissolved DMS in the Ross Sea, Antarctica, Deep-Sea Res. I, 56, 166-177, 2009.
25. Dennett, M. R., Mathot, S., Caron, D. A., Smith Jr., W. O., and Lonsdale, D. J.: Abundance and distribution of phototrophic and heterotrophic nano- and microplankton in the southern Ross Sea, Deep-Sea Res. II, 48, 4019-4037, 2001.
26. DiTullio, G. R. and Smith Jr., W. O.: Relationship between dimethylsulfide and phytoplankton pigment concentrations in the Ross Sea, Antarctica, Deep-Sea Res. I, 42, 873-892, 1995.
27. DiTullio, G. R. and Smith Jr., W. O.: Spatial patterns in phytoplankton biomass and pigment distributions in the Ross Sea, J. Geophys. Res., 101, 18467-18478, 1996.
28. Donat, J. R. and Bruland, K. W.: Direct determination of dissolved cobalt and nickel in seawater by differential pulse cathodic stripping voltammetry preceded by adsorptive collection of cyclohexane-1, 2-dione dioxime complexes, Anal. Chem., 60, 240-244, 1988.
29. Ellwood, M. and Van den Berg, C. M. G.: Zinc speciation in the Northeastern Atlantic Ocean, Mar. Chem., 68, 295-306, 2000.
30. Eppley, R. W.: Temperature and phytoplankton growth in the sea, Fish. Bull., 70, 1063-1085, 1972.
31. Feng, Y., Hare, C. E., Leblanc, K., DiTullio, G. R., Lee, P. A., Wilhelm, S. W., Sun, J., Rose, J. M., Nemcek, N., Benner, I., and Hutchins, D. A.: The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response, Mar. Ecol-Prog. Ser., 388, 13-25, 2009.
32. Firme, G. F., Rue, E. L., Weeks, D. A., Bruland, K. W., and Hutchins, D. A.: Spatial and temporal variability in phytoplankton iron limitation along the California coast and consequences for Si, N, and C biogeochemistry, Global Biogeochem. Cy., 17, 1016, doi:10.1029/2001GB001824, 2003.
33. Fitzwater, S. E., Johnson, K. S., Gordon, R. M., Coale, K. M., and Smith, W. O., Jr: Trace metal concentrations in the Ross Sea and their relationship with nutrients and phytoplankton growth, Deep-Sea Res. II, 47, 3159-3179, 2000.
34. Garrison, D. L.: An overview of the abundance and role of protozooplankton in Antarctic waters, J. Marine Syst., 2, 317-331, 1991.
35. Garrison, D. L. and Gowing, M. M.: Protozooplankton, in: Antarctic Microbiology, edited by: Friedmann, E. I., Wiley-Liss, New York, 123-166, 1993.
36. Garrison, D. L., Gibson, A., Kunze, H., Gowing, M. M., Vickers, C. L., Mathot, S., and Bayre, R. C.: The Ross Sea Polynya Project: diatom- and Phaeocystis-dominated phytoplankton assemblages in the Ross Sea, Antarctica, 1994 and 1995, in: Biogeochemistry of the Ross Sea, edited by: DiTullio, G. R. and Dunbar, R. B., American Geophysical Union, Washington, DC, 53-76, 2003.
37. Genty, B., Briantais, J. M., and Baker, N. R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence, Biochim. Biophys. Acta, 990, 87-92, 1989.
38. Gervais, F., Riebesell, U., and Gorbunov,M. Y.: Changes in primary productivity and chlorophyll a in response to iron fertilization in the Southern Polar Frontal Zone, Limnol. Oceanogr., 47, 1324-1335, 2002.
39. Goldman, J. C. and Carpenter, E. J.: A kinetic approach to the effect of temperature on algal growth, Limnol. Oceanogr., 19, 756-766, 1974.
40. Goldman, J. C.: Temperature effects on steady-state growth, phosphorus uptake, and the chemical composition of a marine phytoplankter, Microb. Ecol., 5, 153-166, 1979.
41. Green, R. M., Kolber, Z. S., Swift, D. G., Tindale, N. W., and Falkowski, P. G.: Physiological limitation of phytoplankton photosynthesis in the Eastern Equatorial Pacific determined from variability in the quantum yield of fluorescence, Limnol. Oceanogr., 39, 1061-1074, 1994.
42. Haberman, K. L., Quetin, L. B., and Ross, R. M.: Diet of the Antarctic krill (Euphausia superba, Dana): I. Comparisons of grazing on Phaeocystis antarctica (Karsten) and Thalassiosira antarctica (Comber), J. Exp. Mar. Biol. Ecol., 283, 79-95, 2003.
43. Haberman, K. L., Ross, R. M., and Quetin, L. B.: Diet of the Antarctic krill (Euphausia superba Dana): II. Selective grazing in mixed phytoplankton assemblages, J. Exp. Mar. Biol. Ecol., 283, 97-113, 2003.
44. Hare, C. E., DiTullio, G. R., Trick, C. G., Wilhelm, S. W., Bruland, K. W., Rue, E. L., and Hutchins, D. A.: Phytoplankton community structure changes following simulated upwelled iron inputs in the Peru upwelling region, Aquat. Microb. Ecol., 38, 269-282, 2005.
45. Hare, C. E., Leblanc, K., DiTullio, G. R., Kudela, R. M., Zhang, Y., Lee, P. A., Riseman, S. F., and Hutchins, D. A.: Consequences of increased temperature and CO2 for phytoplankton community structure in the Bering Sea, Mar. Ecol-Prog. Ser., 352, 9-16, doi:10.3354/meps07182, 2007. (Pubitemid 351211770)
46. Henjes, J., Assmy, P., Klaas, C., Verity, P. G., and Smetacek, V.: Response of microzooplankton (protists and small copepods) to an iron-induced phytoplankton bloom in the Southern Ocean (EisenEx), Deep-Sea Res. I, 54, 363-384, 2007. (Pubitemid 46320260)
47. Hutchins, D. A. and Bruland, K. W.: Iron-limited diatom growth and Si:N uptake ratios in a coastal upwelling regime, Nature, 393, 561-564, 1998.
48. Hutchins, D. A., Campbell, B. J., Cottrell, M. T., Takeda, S., and Cary, S. C.: Response of marine bacterial community composition to iron additions in three iron-limited regimes, Limnol. Oceanogr., 46, 1535-1545, 2001.
49. Hutchins, D. A., Hare, C. E., Weaver, R. S., Zhang, Y., Firme, G. F., DiTullio, G. R., Alm, M. B., Riseman, S. F., Maucher, J. M., Geesey, M. E., Trick, C. G., Smith, G. J., Rue, E. L., Conn, J., and Bruland, K. W.: Phytoplankton iron limitation in the Humboldt Current and Peru Upwelling, Limnol. Oceanogr., 47, 997-1011, 2002.
50. Jacobs, S. S., Giulivi, C. F., and Mele, P. A.: Freshening of the Ross Sea during the late 20th century, Science, 297, 386-389, 2002.
51. Jacobson, D. M. and Anderson, D. M.: Thecate heterotrophic dinoflagellates: feeding behaviors and mechanisms, J. Phycol., 22, 249-258, 1986.
52. Kiene, R. P. and Slezak, D.: Low dissolved DMSP concentrations in seawater revealed by small-volume gravity filtration and dialysis sampling, Limnol. Oceanogr-Meth., 4, 80-95, 2006.
53. Kiene, R. P., Kieber, D. J., Slezak, D., Toole, D. A., del Valle, D. A., Bisgrove, J., Brinkley, J., and Rellinger, A.: Distribution of cycling of dimethylsulfide, dimethylsulfoniopropionate, and dimethylsulfoxide during spring and early summer in the Southern Ocean south of New Zealand, Aquat. Sci., 69, 305-319, 2007.
54. Kolber, Z. S., Barber, R. T., Coale, K. H., Fitzwater, S. E., Greene, R. M., Johnson, K. S., Lindley, S., and Falkowski, G. P.: Iron limitation of the phytoplankton photosynthesis in the equatorial Pacific Ocean, Nature, 371, 145-149, 1994. (Pubitemid 24284011)
55. Leblanc, K., Hare, C. E., Boyd, P. W., Bruland, K. W., Sohst, B., Pickmere, S., Lohan, M. C., Buck, K., Ellwood, M., and Hutchins, D. A.: Fe and Zn effects on the Si cycle and diatom community structure in two contrasting high and low-silicate HNLC areas, Deep-Sea Res. I, 52, 1842-1864, 2005.
56. Leventer, A. R. and Dunbar, R. B.: Factors affecting the distribution of diatoms and other algae in the Ross Sea, J. Geophys. Res., 101, 18489-18500, 1996.
57. - 3 uptake and assimilation: comparison of diatoms and dinoflagellates at several growth temperatures, Mar. Biol., 133, 541-551, 1999.
58. Martin-Jezequel, V., Hildebrand, M., and Brzezinski, M. A.: Silicon metabolism in diatoms: implications for growth, J. Phycol., 36, 821-840, 2000.
59. Martin, J. H., Gordon, R. M., and Fitzwater, S. E.: Iron in Antarctic waters, Nature, 345, 156-158, 1990.
60. Maucher, J. M. and DiTullio, G. R.: Chronic iron limitation in the Ross Sea: Flavodoxin:Ferredoxin ratios in the Ross Sea, in: Biogeochemistry of the Ross Sea, edited by: DiTullio, G. R., and Dunbar, R. B., Antarctic Research Series, AGU Antarctic Research Series, Washington DC, 209-220, 2003.
61. Montes-Hugo, M. A., Doney, S. C., Ducklow, H. W., Fraser, W., Martinson, D., Stammerjohn, S. E., and Schofiield, O.: Recent changes in phytoplankton communities associated with rapid regional climate change along the Western Antarctic Peninsula, Science, 323, 1470-1473, 2009.
62. Noiri, Y., Kudo, I., Kiyosawa, H., Nishioka, J., and Tsuda, A.: Influence of iron and temperature on growth, nutrient utilization ratios and phytoplankton species composition in the western subarctic Pacific Ocean during the SEEDS experiment, Prog. Oceanogr., 64, 149-166, 2005.
63. Olson, R. J., Sosik, H. M., Chekalyuk, A. M., and Shalapyonok, A.: Effects of iron enrichment on phytoplankon in the Southern Ocean during late summer: active fluorescence and flow cytometric analyses, Deep-Sea Res. II, 47, 3181-3200, 2000.
64. Overpeck, J. T., Otto-Bliesner, B. L., and Miller, G. H.: Paleoclimatic evidence for future ice-sheet instability and rapid sea-level rise, Science, 311, 1747-1750, 2006.
65. Paasche, E.: Silicon, in: The physiological ecology of phytoplankton, edited by: Morris, I., Studies in Ecology, University of California Press, Berkeley, CA, 259-284, 1980.
66. Pondaven, P., Gallinari, M., Chollet, S., Bucciarelli, E., Sarthou, G., Schultes, S., and Jean, F.: Grazing-induced changes in cell wall silicification in a marine diatom, Protist, 158, 21-28, 2007.
67. Raiswell, R., Tranter, M., Benning, L. G., Siegert, M., De'ath, R., Huybrechts, P., and Payne, T.: Contributions from glacially derived sediment to the global iron (oxyhydr)oxide cycle: implications for iron delivery to the oceans, Geochim. Cosmochim. Ac., 70, 2765-2780, 2006.
68. Raiswell, R., Benning, L., Tranter, M., and Tulaczyk, S.: Bioavailable iron in the Southern Ocean: the significance of the iceberg conveyor belt, Geochem. T., 9, 7, doi:10.1186/1467-4866-9-7, 2008.
69. Raven, J. A. and Geider, R. J.: Temperature and algal growth, New Phytol., 110, 441-461, 1988.
70. Rellinger, A. N., Kiene, R. P., del Valle, D. A., Kieber, D. J., Slezak, D., Harada, H., Bisgrove, J., and Brinkley, J.: Occurrence and turnover of DMSP and DMS in deep waters of the Ross Sea, Antarctica, Deep-Sea Res. I, 56, 686-702, 2009.
71. Revel-Rolland, M., De Deckker, P., Delmonte, B., Hesse, P. P., Magee, J. W., Basile-Doelsch, I., Grousset, F., and Bosch, D.: Eastern Australia: a possible source of dust in East Antarctica interglacial ice, Earth Planet. Sc. Lett., 249, 1-13, 2006.
72. Rose, J. M. and Caron, D. A.: Does low temperature constrain the growth rates of heterotrophic protists? Evidence and implications for algal blooms in cold waters, Limnol. Oceanogr., 52, 886-895, 2007.
73. Rose, J. M., Feng, Y., Gobler, C. J., Gutierrez, R., Hare, C. E., Leblanc, K., and Hutchins, D. A.: The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom. II. Microzooplankton abundance and grazing, Mar. Ecol-Prog. Ser., 388, 27-40, 2009.
74. Sarmiento, J. L., Hughes, T. M. C., Stouffer, R. J., and Manabe, S.: Simulated response of the ocean carbon cycle to anthropogenic climate warming, Nature, 393, 245-249, 1998.
75. Scharek, R., van Leeuwe, M. A., and de Baar, H. J. W.: Responses of Southern Ocean phytoplankton to the addition of trace metals, Deep-Sea Res. II, 44, 209-227, 1997.
76. Sedwick, P. N. and DiTullio, G. R.: Regulation of algal blooms in Antarctic shelf waters by the release of iron from melting sea ice, Geophys. Res. Lett., 24, 2515-2518, 1997.
77. Sedwick, P. N., DiTullio, G. R., and Mackey, D. J.: Iron and manganese in the Ross Sea, Antarctica: seasonal iron limitation in Antarctic shelf waters, J. Geophys. Res., 105, 11321-11332, 2000.
78. Smith Jr., W. O., Marra, J., Hiscock, M. R., and Barber, R. T.: The seasonal cycle of phytoplankton biomass and primary productivity in the Ross Sea, Antarctica, Deep-Sea Res. II, 47, 3119-3140, 2000.
79. Smith Jr., W. O., Dennett, M. R., Mathot, S., and Caron, D. A.: The temporal dynamics of the flagellated and colonial stages of Phaeocystis antarctica in the Ross Sea, Deep-Sea Res. II, 50, 605-617, 2003.
80. Solorzano, L. and Sharp, J. H.: Determination of total dissolved phosphorus and particulate phosphorus in natural waters, Limnol. Oceanogr., 25, 756-760, 1980.
81. Strom, S., Wolfe, G., Holmes, J., Stecher, H., Shimeneck, C., Lambert, S., and Moreno, E.: Chemical defense in the microplankton I. Feeding and growth rates of heterotrophic protists on the DMSproducing phytoplankter Emiliana huxleyi, Limnol. Oceanogr., 48, 217-229, 2003.
82. Sunda, W. G. and Huntsman, S. A.: Interrelated influence of iron, light, and cell size on growth of marine phytoplankton, Nature, 390, 389-392, 1997.
83. Sunda, W. G., Kieber, D. J., Kiene, R. P., and Huntsman, S. A.: An antioxidant function for DMSP and DMS in marine algae, Nature, 418, 317-320, 2002.
84. Takeda, S.: Influence of iron availability on nutrient consumption ratio of diatoms in oceanic waters, Nature, 393, 774-777, 1998.
85. Thamatrakoln, K. and Hildebrand, M.: Silicon uptake in diatoms revisited: a model for saturable and nonsaturable uptake kinetics and the role of silicon transporters, Plant Physiol., 146, 1397-1407, 2008.
86. Throndsen, J.: Preservation and storage, in: Phytoplankton manual, edited by: Sournia, A., UNESCO, Paris, 69-74, 1978.
87. Twining, B. S., Baines, S. B., Fisher, N. S., and Landry, M. R.: Cellular iron contents of plankton during the Southern Ocean Iron Experiment (SOFeX), Deep-Sea Res. I, 51, 1827-1850, 2004.
88. Twining, B. S., Baines, S. B., Vogt, S., and De Jonge, M.: Exploring ocean biogeochemistry by single-cell microprobe analysis of protist elemental composition, J. Eukaryot. Microbiol., 55, 151-162, 2008.
89. Utermöhl, H.: Zur Vervollkommung der quantitativen phytoplankton-methodik, Mitt. Int. Ver. Limnol., 9, 1-38, 1958.
90. Vaughan, D. G., Marshall, G. J., Connolley, W. M., Parkinson, C., Mulvaney, R., Hodgson, D. A., King, J. C., Pudsey, C. J., and Turner, J.: Recent rapid regional climate warming on the Antarctic Peninsula, Clim. Change, 60, 243-274, 2003.
91. Vogt, M., Steinke, M., Turner, S., Paulino, A., Meyerhöfer, M., Riebesell, U., LeQúeŕe, C., and Liss, P.: Dynamics of dimethylsulphoniopropionate and dimethylsulphide under different CO2 concentrations during a mesocosm experiment, Biogeosciences, 5, 407-419, 2008, http://www.biogeosciences.net/5/407/2008/.
92. Welschmeyer, N. A.: Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and phaeopigments, Limnol. Oceanogr., 39, 1985-1992, 1994.
93. Wilcox, R. R.: Applying contemporary statistical techniques, Academic Press, New York, 608 pp., 2003.
94. Wingenter, O. W., Haase, K. B., Zeigler, M., Blake, D. R., Rowland, F. S., Sive, B. C., Paulino, A., Thyrhaug, R., Larsen, A., Schulz, K., Meyerhofer, M., and Riebesell, U.: Unexpected consequences of increasing CO2 and ocean acidity on marine production of DMS and CH2Cl 1. Potential climate impacts, Geophys. Res. Lett., 34, L05710, doi:10.1029/2006GL028139, 2007. (Pubitemid 46864532)
95. Wolfe, G. V. and Steinke, M.: Grazing-activated production of dimethylsulfide (DMS) by two clones of Emiliania huxleyi, Limnol. Oceanogr., 41, 1151-1160, 1996.
96. Wolfe, G. V., Steinke, M., and Kirst, G. O.: Grazing-activated chemical defence in a unicellular marine alga, Nature, 387, 894-897, 1997.