Characterization and quality control of antibodies used in ChIP assays

Methods in Molecular Biology

Volumn 567, Issue , 2009, Pages 27-43

  Goens, Géraldine ^a   Rusu, Dorina ^a   Bultot, Laurent ^a,b   Goval, Jean Jacques ^a   Magdalena, Juana ^a  

^a Diagenode Sa   (Belgium)

^b UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

Author keywords

Antibody; characterization; chromatin immunoprecipitation; quality control; specificity

Indexed keywords

ORYCTOLAGUS CUNICULUS;

ANTIBODY;

ANIMAL; CHROMATIN IMMUNOPRECIPITATION; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; INSTRUMENTATION; METHODOLOGY; QUALITY CONTROL; RABBIT; REVIEW; STANDARD; WESTERN BLOTTING;

ANIMALS; ANTIBODIES; BLOTTING, WESTERN; CHROMATIN IMMUNOPRECIPITATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; QUALITY CONTROL; RABBITS;

EID: 73949146185     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-414-2_2     Document Type: Article

References (4)

1. Harlow, Ed. and Lane, D. (1998) antibodies: a laboratory manual. cold spring harbor laboratory press, new york.
2. Sarma, K., Nishioka, K. and Reinberg, D. (2004) tips in analyzing antibodies directed against specific histone tail modifications. methods enzymol. 376, 255-269. (Pubitemid 38200184)
3. Burgos, L., Peters, A.H., Opravil, S., Kauer, M., Mechtler, K. and Jenuwein, T. (2004) generation and characterization of methyllysine histone antibodies. methods enzymol. 376, 234-254. (Pubitemid 38200183)
4. Cheung, P. (2004) generation and characterization of antibodies directed against di-modified histones, and comments on antibody and epitope recognition. methods enzymol. 376, 221-234. (Pubitemid 38200182)