A graphical method for determining inhibition constants

Journal of Enzyme Inhibition and Medicinal Chemistry

Volumn 24, Issue 6, 2009, Pages 1288-1290

  Yoshino, Masataka ^a   Murakami, Keiko ^a  

^a AICHI MEDICAL UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Graphical analysis; Inhibition; Inhibition constants; Quotient velocity plot

Indexed keywords

ENZYME INHIBITOR;

ARTICLE; COMPETITIVE INHIBITION; CONTROLLED STUDY; DISSOCIATION CONSTANT; ENZYME INHIBITION; ENZYME INHIBITOR COMPLEX; ENZYME MECHANISM; ENZYME SPECIFICITY; GRAPHICAL METHOD; INHIBITION KINETICS; METHODOLOGY; PRIORITY JOURNAL; VELOCITY;

BINDING SITES; COMPUTER GRAPHICS; ENZYME INHIBITORS; ENZYMES; KINETICS; MATHEMATICS; MODELS, BIOLOGICAL; SUBSTRATE SPECIFICITY;

EID: 73949119611     PISSN: 14756366     EISSN: 14756374     Source Type: Journal    
DOI: 10.3109/14756360902829766     Document Type: Article

References (11)

1. Dixon M. The determination of enzyme inhibitor constants. Biochem J 1953;55:170-171
2. Schlamowitz M, Shaw A, Jackson WT. Limitations of the Dixon plot for ascertaining nature of enzyme inhibition. Tex Rep Biol Med 1969;27:483-488
3. Cornish-Bowden A. A simple graphical method for determining the inhibition constants of mixed, uncompetitive and non-competitive inhibitors. Biochem J 1974;137:143-144
4. Eisenthal R, Cornish-Bowden A. The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters. Biochem J 1974;139:715-720
5. Baici A. The specific velocity plot. A graphical method for determining inhibition parameters for both linear and hyperbolic enzyme inhibitors. Eur J Biochem 1981;119:9-14.
6. Yoshino M. A graphical method for determining inhibition parameters for partial and complete inhibitors. Biochem J 1987;248:815-820
7. Whiteley CG. Mechanistic and kinetic studies of inhibition of enzymes. Cell Biochem Biophys 2000;33:217-225
8. Hunter A, Downs CE. The inhibition of arginase by amino acids. J Biol Chem 1945;157:427-446
9. Chan WW. Combination plots as graphical tools in the study of enzyme inhibition. Biochem J 1995;311:981-985
10. Cortes A, Cascante M, Cardenas ML, Cornish-Bowden A. Relationships between inhibition constants, inhibitor concentrations for 50% inhibition and types of inhibition: new ways of analysing data. Biochem J 2001;357:263-268
11. Wang J, Araki T, Ogawa T, Matsuoka M, Fukuda H. A method of graphically analyzing substrate-inhibition kinetics. Biotechnol Bioeng 1999;62:403-411