Fabrication, characterization and in vitro evaluation of poly(D,L-lactide-co-glycolide) microparticles loaded with polyamidoamine-plasmid DNA dendriplexes for applications in nonviral gene delivery

Journal of Pharmaceutical Sciences

Volumn 99, Issue 1, 2010, Pages 368-384

  Intra, Janjira ^a   Salem, Aliasger K ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

Dendriplex; Formulation; Microparticle; Nonviral gene delivery; Plasmid DNA; Poly(D,L lactide co glycolide) (PLGA); Polyamidoamine (PAMAM); Vaccine

Indexed keywords

PLASMID DNA; POLYAMIDOAMINE; POLYGLACTIN;

ANIMAL CELL; ARTICLE; CELL STRAIN COS7; CELL STRAIN HEK293; CONTROLLED STUDY; CYTOTOXICITY; DRUG FORMULATION; DRUG SYNTHESIS; EVAPORATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PARTICLE SIZE; POROSITY; SURFACE CHARGE; ZETA POTENTIAL;

EID: 73949107821     PISSN: 00223549     EISSN: 15206017     Source Type: Journal    
DOI: 10.1002/jps.21840     Document Type: Article

References (58)

1. Abbas AO, Donovan MD, Salem AK. 2008. Formulating poly(lactide-co- glycolide) particles for plasmid DNA delivery. J Pharm Sci 97:2448-2461.
2. Eldridge JH, Staas JK, Meulbroek JA, Tice TR, Gilley RM. 1991. Biodegradable and biocompatible poly(DL-lactide-co-glycolide) microspheres as an adjuvant for staphylococcal enterotoxin-b toxoid which enhances the level of toxin-neutralizing anti-bodies. Infect Immun 59:2978-2986.
3. O'Hagan DT, Singh M, Ulmer JB. 2004. Microparticles for the delivery of DNA vaccines. Immunol Rev 199:191-200.
4. Zhang XQ, Intra J, Salem AK. 2008. Comparative study of poly(lactic-co-glycolic acid)-poly ethyleneimine-plasmid DNA microparticles prepared using double emulsion methods. J Microencapsul 25:1-12.
5. Walter E, Moelling K, Pavlovic J, Merkle HP. 1999. Microencapsulation of DNA using poly(DL-lactideco-glycolide): Stability issues and release characteristics. J Control Release 61:361-374.
6. Tinsley-Bown AM, Fretwell R, Dowsett AB, Davis SL, Farrar GH. 2000. Formulation of poly(D,L-lactic-co-glycolic acid) microparticles for rapid plasmid DNA delivery. J Control Release 66:229-241.
7. Kasturi SP, Qin H, Thomson KS, El-Bereir S, Cha SC, Neelapu S, Kwak LW, Roy K. 2006. Prophylactic anti-tumor effects in a B cell lymphoma model with DNA vaccines delivered on polyethylenimine (PEI) functionalized PLGA microparticles. J Control Release 113:261-270.
8. Kasturi SP, Sachaphibulkij K, Roy K. 2005. Covalent conjugation of polyethyleneimine on biodegradable microparticles for delivery of plasmid DNA vaccines. Biomaterials 26:6375-6385.
9. Manuel WS, Zheng J, Hornsby PJ. 2001. Transfection by polyethyleneimine-coated microspheres. J Drug Target 9:15-+.
10. Singh M, Briones M, Ott G, O'Hagan D. 2000. Cationic microparticles: A potent delivery system for DNA vaccines. Proc Natl Acad Sci USA 97:811-816.
11. Zhang XQ, Intra J, Salem AK. 2007. Conjugation of polyamidoamine dendrimers on biodegradable microparticles for nonviral gene delivery. Bioconjug Chem 18:2068-2076.
12. Sutton D, Durand R, Shuai XT, Gao JM. 2006. Poly(D,L-lactide-co- glycolide)/poly(ethylenimine) blend matrix system for pH sensitive drug delivery. J Appl Polym Sci 100:89-96.
13. Oster CG, Kissel T. 2005. Comparative study of DNAencapsulation into PLGA microparticles using modified double emulsion methods and spray drying techniques. J Microencapsul 22:235-244.
14. Yun YH, Jiang HL, Chan R, Chen WL. 2005. Sustained release of PEG-g-chitosan complexed DNA from poly(lactide-co-glycolide). J Biomater Sci-Polym Ed 16:1359-1378.
15. Kim IS, Lee SK, Park YM, Lee YB, Shin SC, Lee KC, Oh IJ. 2005. Physicochemical characterization of poly(L-lactic acid) and poly(D,L-lactide-co- glycolide) nanoparticles with polyethylenimine as gene delivery carrier. Int J Pharm 298:255-262.
16. Oster CG, Kim N, Grode L, Barbu-Tudoran L, Schaper AK, Kaufmann SHE, Kissel T. 2005. Cationic microparticles consisting of poly(lactide-co-glycolide) and polyethylenimine as carriers systems for parental DNA vaccination. J Control Release 104:359-377.
17. Bielinska AU, Chen CL, Johnson J, Baker JR. 1999. DNA complexing with polyamidoamine dendrimers: Implications for transfection. Bioconjug Chem 10:843-850.
18. Braun CS, Vetro JA, Tomalia DA, Koe GS, Koe JG, Middaugh CR. 2005. Structure/function relationships of polyamidoamine/DNA dendrimers as gene delivery vehicles. J Pharm Sci 94:423-436.
19. Cheng YY, Xu ZH, Ma ML, Xu TW. 2008. Dendrimers as drug carriers: Applications in different routes of drug administration. J Pharm Sci 97:123-143.
20. Esfand R, Tomalia DA. 2001. Poly(amidoamine) (PAMAM) dendrimers: From biomimicry to drug delivery and biomedical applications. Drug Discov Today 6:427-436.
21. Zhou JH, Wu JY, Hafdi N, Behr JP, Erbacher P, Peng L. 2006. PAMAM dendrimers for efficient siRNA delivery and potent gene silencing. Chem Commun 2362-2364.
22. Tang MX, Szoka FC. 1997. The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes. Gene Ther 4:823-832.
23. Hansen MB, Nielsen SE, Berg K. 1989. Re-examination and further development of a precise and rapid dye method for measuring cell-growth cell kill. J Immunol Methods 119:203-210.
24. Nam HY, Nam K, Hahn HJ, Kim BH, Lim HJ, Kim HJ, Choi JS, Park JS. 2009. BiodegradablePAMAM ester for enhanced transfection efficiency with low cytotoxicity. Biomaterials 30:665-673.
25. Tang MX, Redemann CT, Szoka FC. 1996. In vitro gene delivery by degraded polyamidoamine dendrimers. Bioconjug Chem 7:703-714.
26. Intra J, Salem AK. 2008. Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo. J Control Release 130:129-138.
27. Panyam J, Labhasetwar V. 2003. Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv Drug Deliv Rev 55:329-347.
28. Prabha S, Labhasetwar V. 2004. Critical determinants in PLGA/PLA nanoparticle-mediated gene expression. Pharm Res 21:354-364.
29. Stern M, Ulrich K, Geddes DM, Alton EWFW. 2003. Poly(D,L-lactide-co- glycolide)/DNA microspheres to facilitate prolonged transgene expression in airway epithelium in vitro, ex vivo and in vivo. Gene Ther 10:1282-1288.
30. Bivas-Benita M, Romeijn S, Junginger HE, Borchard G. 2004. PLGA-PEI nanoparticles for gene delivery to pulmonary epithelium. Eur J Pharm Biopharm 58:1-6.
31. De Rosa G, Bochot A, Quaglia F, Besnard M, Fattal E. 2003. A new delivery system for antisense therapy: PLGA microspheres encapsulating oligonucleotide/polyethyleneimine solid complexes. Int J Pharm 254:89-93.
32. De Smedt SC, Demeester J, Hennink WE. 2000. Cationic polymer based gene delivery systems. Pharm Res 17:113-126.
33. Fu HL, Cheng SX, Zhang XZ, Zhuo RX. 2008. Dendrimer/DNA complexes encapsulated functional biodegradable polymer for substratemediated gene delivery. J Gene Med 10:1334-1342.
34. Ribeiro S, Hussain N, Florence AT. 2005. Release of DNA from dendriplexes encapsulated in PLGA nanoparticles. Int J Pharm 298:354-360.
35. Takashima Y, Saito R, Nakajima A, Oda M, Kimura A, Kanazawa T, Okada H. 2007. Spray-drying preparation of microparticles containing cationic PLGA nanospheres as gene carriers for avoiding aggregation of nanospheres. Int J Pharm 343:262-269.
36. Ungaro F, De Rosa G, Quaglia F, Fattal E, La Rotonda MI. 2005. Controlled release of oligonucleotide/polyethyleneimine complexes from PLGA-based microspheres: Potential of spray-drying technique. J Drug Deliv Sci Technol 15:137-143.
37. Zhang XQ, Dahle CE, Weiner GJ, Salem AK. 2007. A comparative study of the antigen-specific immune response induced by co-delivery of CpG ODN and antigen using fusion molecules or biodegradable microparticles. J Pharm Sci 96:3283-3292.
38. Radu DR, Lai CY, Jeftinija K, Rowe EW, Jeftinija S, Lin VSY. 2004. A polyamidoamine dendrimer-capped mesoporous silica nanosphere-based gene transfection reagent. J Am Chem Soc 126:13216-13217.
39. Lee CC, MacKay JA, Frechet JMJ, Szoka FC. 2005. Designing dendrimers for biological applications. Nat Biotechnol 23:1517-1526.
40. Verbaan FJ, Bos GW, Oussoren C, Woodle MC, Hennink WE, Storm G. 2004. A comparative study of different cationic transfection agents for in vivo gene delivery after intravenous administration. J Drug Deliv Sci Technol 14:105-111.
41. Verbaan FJ, Oussoren C, van Dam IM, Takakura Y, Hashida M, Crommelin DJA, Hennink WE, Storm G. 2001. The fate of poly(2-dimethyl amino ethyl)-methacrylate-based polyplexes after intravenous administration. Int J Pharm 214:99-101.
42. Li S, Tseng WC, Stolz DB, Wu SP, Watkins SC, Huang L. 1999. Dynamic changes in the characteristics of cationic lipidic vectors after exposure to mouse serum: Implications for intravenous lipofection. Gene Ther 6:585-594.
43. Duncan R, Izzo L. 2005. Dendrimer biocompatibility and toxicity. Adv Drug Deliv Rev 57:2215-2237.
44. Kuo JHS, Jan MS, Chu HW. 2005. Mechanism of cell death induced by cationic dendrimers in RAW 264.7 murine macrophage-like cells. J Pharm Pharmacol 57:489-495.
45. Malik N, Wiwattanapatapee R, Klopsch R, Lorenz K, Frey H, Weener JW, Meijer EW, Paulus W, Duncan R. 2000. Dendrimers: Relationship between structure and biocompatibility in vitro, and preliminary studies on the biodistribution of I-125-labelled polyamidoamine dendrimers in vivo. J Control Release 65:133-148.
46. Capan Y, Woo BH, Gebrekidan S, Ahmed S, DeLuca PP. 1999. Preparation and characterization of poly(D,L-lactide-co-glycolide) microspheres for controlled release of poly(L-lysine) complexed plasmid DNA. Pharm Res 16:509-513.
47. Munier S, Messai I, Delair T, Verrier B, Ataman-Onal Y. 2005. Cationic PLA nanoparticles for DNA delivery: Comparison of three surface polycations for DNA binding, protection and transfection properties. Colloid Surf B-Biointerfaces 43:163-173.
48. De Rosa G, Quaglia F, La Rotonda MI, Appel M, Alphandary H, Fattal E. 2002. Poly(lactide-co-glycolide) microspheres for the controlled release of oligonucleotide/polyethylenimine complexes. J Pharm Sci 91:790-799.
49. Pistel KF, Kissel T. 2000. Effects of salt addition on the microencapsulation of proteins using W/O/W double emulsion technique. J Microencapsul 17:467-483.
50. Crotts G, Park TG. 1995. Preparation of porous and nonporous biodegradable polymeric hollow microspheres. J Control Release 35:91-105.
51. Thomas M, Klibanov AM. 2003. Non-viral gene therapy: Polycation-mediated DNA delivery. Appl Microbiol Biotechnol 62:27-34.
52. Jevprasesphant R, Penny J, Jalal R, Attwood D, McKeown NB, D'Emanuele A. 2003. The influence of surface modification on the cytotoxicity of PAMAM dendrimers. International Journal of Pharmaceutics 252:263-266. PII S0378-5173(0302)00623-00623.
53. Jevprasesphant R, Penny J, Attwood D, McKeown NB, D'Emanuele A. 2003. Engineering of dendrimer surfaces to enhance transepithelial transport and reduce cytotoxicity. Pharm Res 20:1543-1550.
54. Intra J, Glasgow JA, Mai HQ, Salem AK. 2008. Pulsatile release of biomolecules from polydimethylsiloxane (PDMS) chips with hydrolytically degradable seals. J Control Release 127:280-287.
55. Zhang XQ, Wang XL, Huang SW, Zhuo RX, Liu ZL, Mao HQ, Leong KW. 2005. In vitro gene delivery using polyamidoamine dendrimers with a trimesyl core. Biomacromolecules 6:341-350.
56. Zinselmeyer BH, Mackay SP, Schatzlein AG, Uchegbu IF. 2002. The lower-generation polypropylenimine dendrimers are effective gene-transfer agents. Pharm Res 19:960-967.
57. Ogris M, Brunner S, Schuller S, Kircheis R, Wagner E. 1999. PEGylated DNA/transferrin-PEI complexes: Reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery. Gene Ther 6:595-605.
58. Dash PR, Read ML, Barrett LB, Wolfert M, Seymour LW. 1999. Factors affecting blood clearance and in vivo distribution of polyelectrolyte complexes for gene delivery. Gene Ther 6:643-650.