Rapid identification of Propionibacterium acnes from blood cultures by fluorescence in situ hybridization

Diagnostic Microbiology and Infectious Disease

Volumn 66, Issue 2, 2010, Pages 214-216

  Poppert, Sven ^a   Riecker, Melanie ^b   Essig, Andreas ^b  

^a BERNHARD NOCHT INSTITUTE FOR TROPICAL MEDICINE   (Germany)

^b UNIVERSITY OF ULM   (Germany)

Author keywords

Blood culture; FISH; In situ hybridization; Probe; Propionibacterium; rRNA

Indexed keywords

RNA 16S;

ACCURACY; ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BLOOD CULTURE; CONTROLLED STUDY; CULTURE MEDIUM; FLUORESCENCE IN SITU HYBRIDIZATION; GRAM POSITIVE BACTERIUM; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; PROPIONIBACTERIUM ACNES; SENSITIVITY AND SPECIFICITY;

BACTEREMIA; BLOOD; CLINICAL LABORATORY TECHNIQUES; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; OLIGONUCLEOTIDE PROBES; PROPIONIBACTERIUM ACNES; SENSITIVITY AND SPECIFICITY;

EID: 73749087892     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2009.09.007     Document Type: Article

References (16)

1. Alexeyev O.A., Marklund I., Shannon B., Golovleva I., Olsson J., Andersson C., Eriksson I., Cohen R., and Elgh F. Direct visualization of Propionibacterium acnes in prostate tissue by multicolor fluorescent in situ hybridization assay. J. Clin. Microbiol. 45 (2007) 3721-3728
2. Amann R.I., Binder B.J., Olson R.J., Chisholm S.W., Devereux R., and Stahl D.A. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56 (1990) 1919-1925
3. Arnell K., Cesarini K., Lagerqvist-Widh A., Wester T., and Sjolin J. Cerebrospinal fluid shunt infections in children over a 13-year period: anaerobic cultures and comparison of clinical signs of infection with Propionibacterium acnes and with other bacteria. J. Neurosurg. Pediatr. 1 (2008) 366-372
4. Hiraishi A. Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett. Appl. Microbiol. 15 (1992) 210-213
5. Hugenholtz P., Tyson G.W., and Blackall L.L. Design and evaluation of 16S rRNA-targeted oligonucleotide probes for fluorescence in situ hybridization. Methods Mol. Biol. 179 (2002) 29-42
6. Kempf V.A., Trebesius K., and Autenrieth I.B. Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures. J. Clin. Microbiol. 38 (2000) 830-838
7. Lau S.K., Woo P.C., Fung A.M., Chan K.M., Woo G.K., and Yuen K.Y. Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing. J. Med. Microbiol. 53 (2004) 1247-1253
8. Lee C.C., Lin W.J., Shih H.I., Wu C.J., Chen P.L., Lee H.C., Lee N.Y., Chang C.M., Wang L.R., and Ko W.C. Clinical significance of potential contaminants in blood cultures among patients in a medical center. J. Microbiol. Immunol. Infect. 40 (2007) 438-444
9. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., Jobb G., Forster W., Brettske I., Gerber S., Ginhart A.W., Gross O., Grumann S., Hermann S., Jost R., Konig A., Liss T., Lussmann R., May M., Nonhoff B., Reichel B., Strehlow R., Stamatakis A., Stuckmann N., Vilbig A., Lenke M., Ludwig T., Bode A., and Schleifer K.H. ARB: a software environment for sequence data. Nucleic Acids Res. 32 (2004) 1363-1371
10. Perry A.L., and Lambert P.A. Propionibacterium acnes. Lett. Appl. Microbiol. 42 (2006) 185-188
11. Peters R.P., van Agtmael M.A., Simoons-Smit A.M., Danner S.A., Vandenbroucke-Grauls C.M., and Savelkoul P.H. Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay. J. Clin. Microbiol. 44 (2006) 4186-4188
12. Sampedro M.F., Piper K.E., McDowell A., Patrick S., Mandrekar J.N., Rouse M.S., Steckelberg J.M., and Patel R. Species of Propionibacterium and Propionibacterium acnes phylotypes associated with orthopedic implants. Diagn. Microbiol. Infect. Dis. 64 (2009) 138-145
13. Sohail M.R., Gray A.L., Baddour L.M., Tleyjeh I.M., and Virk A. Infective endocarditis due to Propionibacterium species. Clin. Microbiol. Infect. 15 (2009) 387-394
14. Sfanos K.S., and Isaacs W.B. An evaluation of PCR primer sets used for detection of Propionibacterium acnes in prostate tissue samples. Prostate 68 (2008) 1492-1495
15. Wellinghausen N., Bartel M., Essig A., and Poppert S. Rapid identification of clinically relevant Enterococcus species by fluorescence in situ hybridization. J. Clin. Microbiol. 45 (2007) 3424-3426
16. Yamada T., Eishi Y., Ikeda S., Ishige I., Suzuki T., Takemura T., Takizawa T., and Koike M. In situ localization of Propionibacterium acnes DNA in lymph nodes from sarcoidosis patients by signal amplification with catalysed reporter deposition. J. Pathol. 198 (2002) 541-547