A reliable method to obtain cells of taste buds from fungiform papillae of mice

Acta Histochemica

Volumn 112, Issue 1, 2010, Pages 107-112

  Qin, Yu Mei ^a   Shi, Jin Qin ^a   Zhang, Gen Hua ^b   Deng, Shao Ping ^a   Wang, Teng Hao ^a  

^a ZHEJIANG GONGSHANG UNIVERSITY   (China)

^b CHANGSHU INSTITUTE OF TECHNOLOGY   (China)

Author keywords

Cytokeratin (CK)8; Fungiform papillae; Immunofluorescence; Mice; Scanning electron microscopy; Taste bud cells; Trypsin

Indexed keywords

CARBON DIOXIDE; CHEMICAL COMPOUND; COLLAGENASE; DISPASE; EDETIC ACID; TRYPAN BLUE; TRYPSIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL FUNCTION; CELL SEPARATION; CELL STRUCTURE; CELL SUSPENSION; CELL TYPE; CELL VIABILITY; EPITHELIUM; FUNGIFORM PAPILLAE; IMMUNOFLUORESCENCE; INCUBATION TIME; MALE; MOUSE; NONHUMAN; RELIABILITY; SCANNING ELECTRON MICROSCOPY; TASTE BUD; TONGUE;

ANIMALS; CELL SEPARATION; EPITHELIUM; FLUORESCENT ANTIBODY TECHNIQUE; KERATIN-8; MALE; MICE; MICE, INBRED ICR; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, SCANNING; TASTE BUDS;

MUS;

EID: 73749084589     PISSN: 00651281     EISSN: 16180372     Source Type: Journal    
DOI: 10.1016/j.acthis.2008.09.007     Document Type: Article

References (18)

1. Béhé P., DeSimone J.A., Avenet P., and Lindemann B. Membrane currents in taste cells of the rat fungiform papillae. Evidence for two types of Ca currents and inhibition of K currents by saccharin. J Gen Physiol 96 (1990) 1061-1084
2. Bigiani A. Mouse taste cells with glia-like membrane properties. J Neurophysiol 85 (2001) 1552-1560
3. Clapp T.R., Medler K.F., Damak S., Margolskee R.F., and Kinnamon S.C. Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25. BMC Biol 4 (2006) 7
4. DeFazio R.A., Dvoryanchikov G., Maruyama Y., Kim J.W., Pereira E., Roper S.D., et al. Separate populations of receptor cells and presynaptic cells in mouse taste buds. J Neurosci 26 (2006) 3971-3980
5. El-Yassimi A., Hichami A., Besnard P., and Khan N.A. Linoleic acid induces calcium signaling, Src kinase phosphorylation, and neurotransmitter release in mouse CD36-positive gustatory cells. J Biol Chem 283 (2008) 12949-12959
6. Herness M.S. A dissociation procedure for mammalian taste cells. Neurosci Lett 106 (1989) 60-64
7. Huang Y.J., and Lu K.S. Unilateral innervation of guinea pig vallate taste buds as determined by glossopharyngeal neurectomy and HRP neural tracing. J Anat 189 (1996) 315-324
8. Kinnamon S.C., Cummings T.A., and Roper S.D. Isolation of single taste cells from lingual epithelium. Chem Senses 13 (1988) 355-366
9. Knapp L., Lawton A., Oakley B., Wong L., and Zhang C.X. Keratins as markers of differentiated taste cells of the rat. Differentiation 58 (1995) 341-349
10. Medler K.F., Margolskee R.F., and Kinnamon S.C. Electrophysiological characterization of voltage-gated currents in defined taste cell types of mice. J Neurosci 23 (2003) 2608-2617
11. Misaki A.M., Ryoko H., and Yasufumi E. Cytokeratin 14 expressed in immature cells in rat taste buds. J Mol Histol 39 (2008) 193-199
12. Seta Y.J., Harada H., and Toyoshima K. Isolation, partial purification, and ultrastructure of taste bud cells from rabbit foliate papillae. Neurosci Lett 227 (1997) 61-64
13. Spielman A.I., Mody I., Brand J.G., Whitney G., MacDonald J.F., and Salter M.W. A method for isolating and patch-clamping single mammalian taste receptor cells. Brain Res 503 (1989) 326-329
14. Stone L.M., Finger T.E., Tam P.P., and Tan S.S. Taste receptor cells arise from local epithelium, not neurogenic ectoderm. Proc Natl Acad Sci USA 92 (1995) 1916-1920
15. Yang R., Crowley H.H., Rock M.E., and Kinnamon J.C. Taste cells with synapses in rat circumvallate papillae display SNAP-25-like immunoreactivity. J Comp Neurol 424 (2000) 205-215
16. Yee C.L., Yang R., Böttger B., Finger T.E., and Kinnamon J.C. "Type III" cells of rat taste buds: immunohistochemical and ultrastructural studies of neuron-specific enolase, protein gene product 9.5, and serotonin. J Comp Neurol 440 (2001) 97-108
17. Zhang C., and Oakley B. The distribution and origin of keratin 20-containing taste buds in rat and human. Differentiation 61 (1996) 121-127
18. Zhang Z., Zhao Z., Margolskee R., and Liman E. The transduction channel TRPM5 is gated by intracellular calcium in taste cells. J Neurosci 27 (2007) 5777-5786