Blood donor screening with cobas s 201/cobas TaqScreen MPX under routine conditions at German Red Cross institutes

Vox Sanguinis

Volumn 98, Issue 1, 2010, Pages 37-46

  Schmidt, M ^a   Pichl, L ^b   Jork, C ^c   Hourfar, M K ^a   Schottstedt, V ^b   Wagner, F F ^c   Seifried, E ^a   Muller T H ^c   Bux, J ^b   Saldanha, J ^d  

^a GOETHE UNIVERSITY   (Germany)

^b Central Lab   (Germany)

^c Institute Springe   (Germany)

^d ROCHE MOLECULAR SYSTEMS   (United States)

Author keywords

MPX; Real time MP NAT; S 201

Indexed keywords

VIRUS DNA; VIRUS RNA;

ARTICLE; BLOOD DONOR; CONTAMINATION; GENOTYPE; GERMANY; HEPATITIS B VIRUS; HEPATITIS C VIRUS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; MAJOR CLINICAL STUDY; NUCLEIC ACID AMPLIFICATION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RED CROSS; REPRODUCIBILITY; SCREENING; SENSITIVITY AND SPECIFICITY; VIRUS LOAD;

AUTOMATIC DATA PROCESSING; AUTOMATION; BLOOD DONORS; GERMANY; HEPACIVIRUS; HEPATITIS B VIRUS; HIV-1; HUMANS; MASS SCREENING; RED CROSS; SENSITIVITY AND SPECIFICITY; VIRUS DISEASES;

EID: 73649136841     PISSN: 00429007     EISSN: 14230410     Source Type: Journal    
DOI: 10.1111/j.1423-0410.2009.01219.x     Document Type: Article

References (18)

1. Roth WK, Weber M, Seifried E : Feasibility and efficacy of routine PCR screening of blood donations for hepatitis C virus, hepatitis B virus, and HIV-1 in a blood-bank setting. Lancet 1999 353 : 359 363
2. Coste J, Reesink HW, Engelfriet CP, Laperche S, Brown S, Busch MP, Cuijpers HT, Elgin R, Ekermo B, Epstein JS, Flesland O, Heier HE, Henn G, Hernandez JM, Hewlett IK, Hyland C, Keller AJ, Krusius T, Levicnik-Stezina S, Levy G, Lin CK, Margaritis AR, Muylle L, Niederhauser C, Pastila S, Pillonel J, Pineau J, van der Poel CL, Politis C, Roth WK, Sauleda S, Seed CR, Sondag-Thull D, Stramer SL, Strong M, Vamvakas EC, Velati C, Vesga MA, Zanetti A : Implementation of donor screening for infectious agents transmitted by blood by nucleic acid technology: update to 2003. Vox Sang 2005 88 : 289 303
3. Stramer SL, Glynn SA, Kleinman SH, Strong DM, Caglioti S, Wright DJ, Dodd RY, Busch MP : Detection of HIV-1 and HCV infections among antibody-negative blood donors by nucleic acid-amplification testing. N Engl J Med 2004 351 : 760 768
4. Roth WK, Seifried E : The German experience with NAT. Transfus Med 2002 12 : 255 258
5. Hourfar MK, Jork C, Schottstedt V, Weber-Schehl M, Brixner V, Busch MP, Geusendam G, Gubbe K, Mahnhardt C, Mayr-Wohlfart U, Pichl L, Roth WK, Schmidt M, Seifried E, Wright DJ : Experience of German Red Cross blood donor services with nucleic acid testing: results of screening more than 30 million blood donations for human immunodeficiency virus-1, hepatitis C virus, and hepatitis B virus. Transfusion 2008 48 : 1558 1566
6. Chevaliez S, Bouvier-Alias M, Laperche S, Pawlotsky JM : Performance of the Cobas AmpliPrep/Cobas TaqMan real-time PCR assay for hepatitis B virus DNA quantification. J Clin Microbiol 2008 46 : 1716 1723
7. Roth WK, Buhr S, Drosten C, Seifried E : NAT and viral safety in blood transfusion. Vox Sang 2000 78 (Suppl. 2 257 259
8. Roth WK, Weber M, Buhr S, Drosten C, Weichert W, Sireis W, Hedges D, Seifried E : Yield of HCV and HIV-1 NAT after screening of 3.6 million blood donations in central Europe. Transfusion 2002 42 : 862 868
9. Koppelman MH, Sjerps MC, Reesink HW, Cuypers HT : Evaluation of COBAS AmpliPrep nucleic acid extraction in conjunction with COBAS AmpliScreen HBV DNA, HCV RNA and HIV-1 RNA amplification and detection. Vox Sang 2005 89 : 193 200
10. Margaritis AR, Brown SM, Seed CR, Kiely P, D'Agostino B, Keller AJ : Comparison of two automated nucleic acid testing systems for simultaneous detection of human immunodeficiency virus and hepatitis C virus RNA and hepatitis B virus DNA. Transfusion 2007 47 : 1783 1793
11. Wiedmann M, Kluwick S, Walter M, Fauchald G, Howe J, Bronold M, Zauke M : HIV-1, HCV and HBV seronegative window reduction by the new Roche cobas TaqScreen MPX test in seroconverting donors. J Clin Virol 2007 39 : 282 287
12. Kretzschmar E, Chudy M, Nubling CM, Ross RS, Kruse F, Trobisch H : First case of hepatitis C virus transmission by a red blood cell concentrate after introduction of nucleic acid amplification technique screening in Germany: a comparative study with various assays. Vox Sang 2007 92 : 297 301
13. Schmidt M, Korn K, Nübling CM, Chudy M, Kress J, Horst HA, Geusendam G, Hennig H, Sireis W, Rabenau HF, Doerr HW, Berger A, Hourfar MK, Gubbe K, Karl A, Fickenscher H, Tischer BK, Babiel R, Seifried E, Gürtler L : First transmission of HIV-1 by a cellular blood product after mandatory nucleic acid screening in Germany. Transfusion, in press.
14. Fiebig EW, Wright DJ, Rawal BD, Garrett PE, Schumacher RT, Peddada L, Heldebrant C, Smith R, Conrad A, Kleinman SH, Busch MP : Dynamics of HIV viremia and antibody seroconversion in plasma donors: implications for diagnosis and staging of primary HIV infection. AIDS 2003 17 : 1871 1879
15. Fang CT, Field SP, Busch MP, Heyns Adu P : Human immunodeficiency virus-1 and hepatitis C virus RNA among South African blood donors: estimation of residual transfusion risk and yield of nucleic acid testing. Vox Sang 2003 85 : 9 19
16. Vermeulen M, Lelie N, Sykes W, Crookes R, Swanevelder J, Gaggia L, Le Roux M, Kuun E, Gulube S, Reddy R : Impact of individual-donation nucleic acid testing on risk of human immunodeficiency virus, hepatitis B virus, and hepatitis C virus transmission by blood transfusion in South Africa. Transfusion 2009 49 : 1115 1125
17. Assal A, Barlet V, Deschaseaux M, Dupont I, Gallian P, Guitton C, Morel P, David B, De Micco P : Comparison of the analytical and operational performance of two viral nucleic acid test blood screening systems: Procleix Tigris and cobas s 201. Transfusion 2009 49 : 289 300
18. Linauts S, Saldanha J, Strong DM : PRISM hepatitis B surface antigen detection of hepatits B virus minipool nucleic acid testing yield samples. Transfusion 2008 48 : 1376 1382