Overexpression, isolation, and spectroscopic characterization of the bidirectional [NiFe] hydrogenase from Synechocystis sp. PCC 6803

Journal of Biological Chemistry

Volumn 284, Issue 52, 2009, Pages 36462-36472

  Germer, Frauke ^a   Zebger, Ingo ^b   Saggu, Miguel ^b   Lendzian, Friedhelm ^b   Schulz, Rüdiger ^a   Appel, Jens ^c  

^a UNIVERSITY OF KIEL   (Germany)

^b TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^c ARIZONA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

[NIFE]-HYDROGENASE; ACTIVE SITE; C TERMINUS; CATALYTIC AMOUNTS; CYANOBACTERIUM SYNECHOCYSTIS; ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPY; FOURIER TRANSFORM INFRARED; HYDROGENASE ACTIVITY; HYDROGENASES; IRON-SULFUR CLUSTERS; OVER-EXPRESSION; PURIFIED ENZYME; REDOX STATE; REDUCTIVE ACTIVATION; SPECTROSCOPIC CHARACTERIZATION; SYNECHOCYSTIS; SYNECHOCYSTIS SP. PCC 6803; WILD TYPES;

AFFINITY CHROMATOGRAPHY; CARBON MONOXIDE; CHROMATOGRAPHIC ANALYSIS; CYANIDES; ELECTRON SPIN RESONANCE SPECTROSCOPY; FOURIER TRANSFORM INFRARED SPECTROSCOPY; PARAMAGNETIC MATERIALS; PARAMAGNETIC RESONANCE; PARAMAGNETISM; PURIFICATION; SPECTROSCOPY; SULFUR;

ENZYMES;

BACTERIAL PROTEIN; BIDIRECTIONAL HYDROGENASE; CARBON MONOXIDE; CYANIDE; HYDROGEN; HYDROGENASE; IRON; NICKEL; PROTEIN HOXE; PROTEIN HYPA; PROTEIN HYPB; PROTEIN HYPC; PROTEIN HYPD; PROTEIN HYPE; PROTEIN HYPF; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SULFUR; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM ISOLATION; CATALYSIS; ELECTRON SPIN RESONANCE; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME PURIFICATION; GENE INSERTION; GENE OVEREXPRESSION; INFRARED SPECTROSCOPY; NONHUMAN; NOSTOC; PHOTOTROPH; PRIORITY JOURNAL; PROMOTER REGION; SPECTROSCOPY; SYNECHOCYSTIS;

BACTERIAL PROTEINS; ELECTRON SPIN RESONANCE SPECTROSCOPY; ENZYME ACTIVATION; GENE EXPRESSION; HYDROGENASE; IRON; NADP; NICKEL; NOSTOC; OXIDATION-REDUCTION; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; SYNECHOCYSTIS;

NOSTOC SP. PCC 7120; SYNECHOCYSTIS; SYNECHOCYSTIS SP.; SYNECHOCYSTIS SP. PCC 6803;

EID: 73649123872     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.028795     Document Type: Article

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