DNA isolation, optimization of ISSR-PCR system and primers screening of Scutellaria baicalensis

Journal of Medicinal Plants Research

Volumn 3, Issue 11, 2009, Pages 898-901

  Guo, H B ^a   Huang, K Y ^b   Zhou, T S ^b   Wu, Q H ^b   Zhang, Y J ^a   Liang, Z S ^a  

^a NORTHWEST A F UNIVERSITY   (China)

^b FUDAN UNIVERSITY   (China)

Author keywords

DNA isolation; ISSR; Optimal PCR condition; Primer screening; Scutellaria baicalensis

Indexed keywords

DEOXYNUCLEOSIDE TRIPHOSPHATE; MAGNESIUM ION; NUCLEOSIDE TRIPHOSPHATE; PRIMER DNA; TAQ POLYMERASE; UNCLASSIFIED DRUG;

ALGORITHM; ARTICLE; DNA DETERMINATION; DNA ISOLATION; DNA SEQUENCE; DNA TEMPLATE; GENETIC SCREENING; GENETIC VARIABILITY; INTER SIMPLE SEQUENCE REPEAT; ISOLATION PROCEDURE; NONHUMAN; PLANT GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SCUTELLARIA BAICALENSIS;

SCUTELLARIA BAICALENSIS;

EID: 73649120294     PISSN: 19960875     EISSN: 19960875     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Feng XF, Hu SL, Guo BL, Li JS, Yan YN (2002). A preliminary study on genetic diversity of populations of Scutellaria baicalensis Georgi (Labiatae). World Sci. Tech-Modern Trad. Chin. Med. 4(4): 38-43.
2. Guo HB, Lu BR, Wu QH, Chen JK, Zhou TS (2007). Abundant genetic diversity in cultivated Codonopsis pilosula populations revealed by RAPD polymorphisms. Genet. Resour. Crop Evol. 54: 917-924.
3. Hong DY, Lian YS, Chen LD (1983). Flora of China. Academic Press, Beijing, China 65(2): 194-196.
4. Liu G, Ma J, Chen Y, Tian Q, Shen Y, Wang X, Chen B, Yao S (2009). Investigation of flavonoid profile of Scutellaria bacalensis Georgi by high performance liquid chromatography with diode array detection and electrospray ion trap mass spectrometry. J. Chromatogr. A 1216: 4809-4814.
5. National Committee of Pharmacopoeia (2005). China Pharmacopoeia. Chemical Industry Press, Beijing, China p.211.
6. Shao AJ, Li X, Huang LQ, Lin SF, Chen J (2006). RAPD analysis of Scutellaria baicalensis from different germplasms. China J. Chin. Mat. Med. 31(6): 452-455.??
7. Su S, He CM, Li LC, Chen JK, Zhou TS (2008). Genetic characterization and phytochemical analysis of wild and cultivated populations of Scutellaria baicalensis. Chem. Biodivers. 5: 1353-1363.
8. Yao H, Zhao Y, Chen DF, Chen JK, Zhou TS (2008). ISSR primer screening and preliminary evaluation of genetic diversity in wild populations of Glycyrrhiza uralensis. Biol. Plantarum 52: 117-120.
9. Zietkiewicz E, Rafalski A, Labuda D (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176-183.