A simplified culture and polymerase chain reaction identification assay for quality control performance testing of stem cell media products

Cytotherapy

Volumn 11, Issue 6, 2009, Pages 761-767

  Boucher, Shayne ^a   Lakshmipathy, Uma ^b   Vemuri, Mohan ^a  

^a Life Technologies Inc   (United States)

^b Life Technologies   (United States)

Author keywords

Adipogenesis; Chondrogenesis; Differentiation; Mesenchymal stromal cell; Microarray; Osteogenesis

Indexed keywords

FATTY ACID BINDING PROTEIN 4; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE;

ADIPOGENESIS; ARTICLE; ASSAY; CELL CULTURE; CELL DIFFERENTIATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; MESENCHYMAL STROMAL CELL; MICROARRAY ANALYSIS; MULTIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROGENY; QUALITY CONTROL; SENSITIVITY AND SPECIFICITY; STEM CELL; VALIDATION PROCESS;

EID: 73649109545     PISSN: 14653249     EISSN: 14772566     Source Type: Journal    
DOI: 10.3109/14653240903052861     Document Type: Article

References (8)

1. Friedenstein AJ, Piatetzky-Shapiro II, Petrakova KV. Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol 1966;16:381-390
2. Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet 1970;3:393-403.
3. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143-147
4. Ng F, Boucher S, Koh S, Sastry KS, Chase L, Lakshmipathy U et al. PDGF, TGF-b and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional prof ling can identify markers and signaling pathways important in differentiation of MSC into adipogenic, chondrogenic and ostoegenic lineages. Blood 2008;112:295-307.
5. Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 1997;64:295-312.
6. Mackay AM, Beck SC, Murphy JM, Barry FP, Chichester CO, Pittenger M F. Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue Eng 1998;4 415-428
7. Nakamura T, Shiojima S, Hirai Y, Iwama T, Tsuruzoe N, Hirasawa A et al. Temporal gene expression changes during adipogenesis in human mesenchymal stem cells. Biochem Biophys Res Comm 2003;303:306-312
8. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006;8:315-317