Characterization of mitosis-specific phosphorylation of tumor-associated microtubule-associated protein

Experimental and Molecular Medicine

Volumn 41, Issue 11, 2009, Pages 832-840

  Hong, Kyung Uk ^a   Kim, Hyun Jun ^a   Bae, Chang Dae ^a   Park, Joobae ^a  

^a Sungkyunkwan University School of Medicine   (South Korea)

Author keywords

Antibodies, polyclonal; Cell cycle; CKAP2 protein, human; Mitosis; Phosphorylation

Indexed keywords

ALANINE; ANTIBODY; PROTEIN; THREONINE; TUMOR ASSOCIATED MICROTUBULE ASSOCIATED PROTEIN; UNCLASSIFIED DRUG;

ANAPHASE; ARTICLE; CELL CYCLE; CELL STRAIN HEK293; CELLULAR DISTRIBUTION; FEMALE; HELA CELL; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOFLUORESCENCE TEST; IMMUNOREACTIVITY; METAPHASE; MITOSIS; PROPHASE; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; SITE DIRECTED MUTAGENESIS;

EID: 73649091133     PISSN: 12263613     EISSN: 20926413     Source Type: Journal    
DOI: 10.3858/emm.2009.41.11.089     Document Type: Article

References (15)

1. Bae CD, Sung YS, Jeon SM, Suh Y, Yang HK, Kim YI, Park KH, Choi J, Ahn G, Park J. Up-regulation of cytoskeletal-associated protein 2 in primary human gastric adenocarcinomas. J Cancer Res Clin Oncol 2003;129:621-30
2. Blangy A, Lane HA, d'Hérin P, Harper M, Kress M, Nigg EA. Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell 1995;83:1159-69
3. Clute P, Pines J. Temporal and spatial control of cyclin B1 destruction in metaphase. Nat Cell Biol 1999;1:82-7
4. Eichmuller S, Usener D, Dummer R, Stein A, Thiel D, Schadendorf D. Serological detection of cutaneous T-cell lymphoma-associated antigens. Proc Natl Acad Sci USA 2001;98:629-34
5. Hendzel MJ, Wei Y, Mancini MA, Van Hooser A, Ranalli T, Brinkley BR, Bazett-Jones DP, Allis CD. Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation. Chromosoma 1997;106:348-60
6. Hong KU, Park YS, Seong YS, Kang D, Bae CD, Park J. Functional importance of the anaphase-promoting complex-Cdh1-mediated degradation of TMAP/CKAP2 in regulation of spindle function and cytokinesis. Mol Cell Biol 2007;27:3667-81
7. Hong KU, Choi YB, Lee JH, Kim HJ, Kwon HR, Seong YS, Kim HT, Park J, Bae CD, Hong KM. Transient phosphorylation of tumor associated microtubule associated protein (TMAP)/cytoskeleton associated protein 2 (CKAP2) at Thr-596 during early phases of mitosis. Exp Mol Med 2008;40:377-86
8. Hong KU, Kim E, Bae CD, Park J. TMAP/CKAP2 is essential for proper chromosome segregation. Cell Cycle 2009a;8:314-24
9. Hong KU, Kim HJ, Kim HS, Seong YS, Hong KM, Bae CD, Park J. Cdk1-cyclin B1-mediated phosphorylation of tumor-associated microtubule-associated protein/cytoskeleton associated protein 2 in mitosis. J Biol Chem 2009b;284:16501-12
10. Jeon SM, Choi B, Hong KU, Kim E, Seong YS, Bae CD, Park J. A cytoskeleton-associated protein, TMAP/CKAP2, is involved in the proliferation of human foreskin fibroblasts. Biochem Biophys Res Commun 2006;348:222-8
11. Jin Y, Murakumo Y, Ueno K, Hashimoto M, Watanabe T, Shimoyama Y, Ichihara M, Takahashi M. Identification of a mouse cytoskeleton-associated protein, CKAP2, with microtubule-stabilizing properties. Cancer Sci 2004;95:815-21
12. Maouche-Chretien L, Deleu N, Badoual C, Fraissignes P, Berger R, Gaulard P, Romeo PH, Leroy-Viard K. Identification of a novel cDNA, encoding a cytoskeletal associated protein, differentially expressed in diffuse large B cell lymphomas. Oncogene 1998;17:1245-51
13. Mishima M, Pavicic V, Grüneberg U, Nigg EA, Glotzer M. Cell cycle regulation of central spindle assembly. Nature 2004;430:908-13
14. Ohsugi M, Tokai-Nishizumi N, Shiroguchi K, Toyoshima YY, Inoue J, Yamamoto T. Cdc2-mediated phosphorylation of Kid controls its distribution to spindle and chromosomes. EMBO J 2003;22:2091-103
15. Whitfield ML, Sherlock G, Saldanha AJ, Murray JI, Ball CA, Alexander KE, Matese JC, Perou CM, Hurt MM, Brown PO, Botstein D. Identification of genes periodically expressed in the human cell cycle and their expression in tumors. Mol Biol Cell 2002;13:1977-2000