Detection of increased amounts of cell-free fetal DNA with short PCR amplicons

Clinical Chemistry

Volumn 56, Issue 1, 2010, Pages 136-138

  Sikora, Aleksandra ^a   Zimmermann, Bernhard G ^b   Rusterholz, Corinne ^a   Birri, Daniella ^a   Kolla, Varaprasad ^a   Lapaire, Olav ^a   Hoesli, Irene ^a   Kiefer, Vivian ^a   Jackson, Laird ^c   Hahn, Sinuhe ^a  

^a UNIVERSITY WOMEN S HOSPITAL   (Switzerland)

^b FLUIDIGM CORPORATION   (United States)

^c DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

AMPLICON; ARTICLE; BLOOD SAMPLING; CELL FREE SYSTEM; CONTROLLED STUDY; DNA DETERMINATION; DNA TEMPLATE; FEMALE; FETUS; FIRST TRIMESTER PREGNANCY; GENE LOCUS; GENE SEQUENCE; HUMAN; MAJOR CLINICAL STUDY; MALE; MATERNAL PLASMA; POLYMERASE CHAIN REACTION; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDY; Y CHROMOSOME;

DNA; FEMALE; FETUS; HUMANS; MALE; MATERNAL-FETAL EXCHANGE; POLYMERASE CHAIN REACTION; PREGNANCY; RETROSPECTIVE STUDIES;

EID: 73449147027     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2009.132951     Document Type: Article

References (10)

1. Lo YMD, Chiu RW. Prenatal diagnosis: progress through plasma nucleic acids. Nat Rev Genet 2007;8:71-7.
2. Hahn S, Chitty LS. Noninvasive prenatal diagnosis: current practice and future perspectives. Curr Opin Obstet Gynecol 2008;20:146 -51.
3. Zimmermann BG, Grill S, Holzgreve W, Zhong XY, Jackson LG, Hahn S. Digital PCR: a powerful new tool for noninvasive prenatal diagnosis? Prenat Diagn 2008;28:1087-93.
4. Lo YMD, Chiu RWK. Next-generation sequencing of plasma/serum DNA: an emerging research and molecular diagnostic tool. Clin Chem 2009;55: 607-8.
5. Zimmermann B, El-Sheikhah A, Nicolaides K, Holzgreve W, Hahn S. Optimized real-time quantitative PCR measurement of male fetal DNA in maternal plasma. Clin Chem 2005;51:1598-604.
6. Hahn S, Chitty LS. Non-invasive prenatal diagnosis: implications for antenatal diagnosis and the management of high-risk pregnancies. Semin Fetal Neonatal Med 2008;13:55- 6.
7. Lun FMF, Chiu RWK, Chan KCA, Leung TY, Lau TK, YMD Lo. Microfluidics digital PCR reveals a higher than expected fraction of fetal DNA in maternal plasma. Clin Chem 2008;54:1664 -72.
8. Chan KCA, Zhang J, Hui ABY, Wong N, Lau TK, Leung TN, et al. Size distributions of maternal and fetal DNA in maternal plasma. Clin Chem 2004; 50:88 -92.
9. Li Y, Zimmermann B, Rusterholz C, Kang A, Holzgreve W, Hahn S. Size separation of circulatory DNA in maternal plasma permits ready detection of fetal DNA polymorphisms. Clin Chem 2004; 50:1002-11.
10. Zhang Y, Zhang D, Li W, Chen J, Peng Y, Cao W. A novel real-time quantitative PCR method using attached universal template probe. Nucleic Acids Res 2003;31:e123.