Isolation and molecular characterization of cry4 harbouring Bacillus thuringiensis isolates from Pakistan and mosquitocidal activity of their spores and total proteins

Pakistan Journal of Zoology

Volumn 42, Issue 1, 2010, Pages 1-15

  Bukhari, Dilara Abbas ^a   Shakoori, Abdul Rauf ^a  

^a UNIVERSITY OF THE PUNJAB   (Pakistan)

Author keywords

Anopheles stephensi; Bacillus thuringiensis; Bioinsecticide; Cry4 gene; Insecticidal activity; Total protein profile of B.t. isolates

Indexed keywords

BACTERIUM; BIOASSAY; BIOLOGICAL CONTROL; BIOPESTICIDE; DNA; GENE; ISOLATED POPULATION; MOLECULAR ANALYSIS; PROTEIN; TOXICITY;

ANIMALIA; ANOPHELES STEPHENSI; BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS); BOS; TRITICUM AESTIVUM;

EID: 73449125472     PISSN: 00309923     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (64)

1. ALBEROLA, T.M., APTOSOGLOU, S., ARSENAKIS, M, BEL, Y., DELRIO, G., ELLAR, D.J., FERRÉ, J., GRANERO, F., GUTTMANN, D.M., KOLIAIS, S., MARTÍNEZ-SEBASTIÁN, γ. M.J., PROTA;. R.., RUBINO, S., SATTA, A., SCARPELLINI, G., SIVROPOULOU, A. AND VASARA, E., 1999. Insecticidal activity of srains of Bacillus ihuringiensis on larvae and adults of Bactrocera oleae Gmelin (Dipt. Tephritidae). J. Inverteb. Paihol.,74: 127-136.
2. ANDRUP, L, SMIDT, L., ANDERSEN, K. AND BOE, L., 1998. Kinetics of conjugate transfer: A study of plasmid pXO from Bacillus ihuringiensis subsp israelensis. Plasmid, 40:30-43.
3. ARONSON, A.I., BECKMAN, W. AND DUNN, P., 1986. Bacillus ihuringiensis and related insect pathogens. Microbiol. Rev., 50: 1-24.
4. BAIRD, J.K., 2000. Resurgent malaria at the millennium: control strategies in crisis. Drugs 59:719-743.
5. BALARAMAN, K., 2005. Occurrence and diversity of mosquitocidal strains of Bacillus thuriungiensis. J. Vect. Borne Dis.. 42: 81-86.
6. BECHTEL, D.B. AND BULLA, L.A. JR., 1976. Electron microscope study of sporulation and parasporal crystal formation in Bacillus ihuringiensis. J Bad., 127: 1472- 1481.
7. BEN-DOV, E., EINAV, M., PELLEG, N., BOUSSIBA, S. AND ZARIYSKY, A., 1996. Resriction map of the 125-kilobase of Bacillus ihuringiensis subsp. israelensis carrying the genes that encode delta endotoxins active against mosquito larvae. Appl. environ. Microbiol., 62: 3140-3145.
8. BEN-DOV, E., ZAR1TSKY, A., DAHAN, E., BARAK, Z., SINAI, R., MANASHEROB, R., KHAMRAEV, A., TROITSKAYA, E., DUBITSKY, A., BEREZINA, N. AND MARGAL1TH, Y., 1997. Extended screening by PCR for seven cry-group genes from field-collected strains of Bacillus ihuringiensis. Appl. environ. Microbiol., 63: 4883-4890.
9. BENINTENDE, G.B., LOPEZ-MEZA, J.E., COZZI1, J.G. AND IBARRA, J.E., 1999. Novel non-toxic isolates of Bacillus thuringiensis. Lett. appl. Microbiol., 29: 151- 155.
10. BRAVO, A., SARABIA, S., LOPEZ, L., ONTIVEROS, H, ABARCA, C, ORTIZ, A., ORTIZ, M., LINA, L., VILLALOBOS, F.J., PENA, G., NUNEZ-VALDEZ, M.E., SOBERON, M. AND QUINTERO, R., 1998. Characterization of cry genes in a Mexican Bacillus thuringiensis strain collection. Appl. environ. Microbiol., 64: 4965-4972.
11. CAROZZ1, N.B., KRAMER, V.C., WARREN, G.W., EVOLA, S. AND KOZIEL, M.G., 1991. Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Appl. environ. Microbiol., 57: 3057-3061.
12. CAVADOS, C.F.G., FONSECA, R.N., CHAVES, J.Q., ARAUJO-COUTINHO, C.J.P.C. AND RAB1NOVITCH, L., 2005. A new black fly isolate of Bacillus thuringiensis autoagglutinating strain highly toxic to Simulium pertinax (Kollar) (Diptera, Simuliidae) larvae. Mem. Inst. Oswaldo. Cruz, Rio de Janeiro, 100: 795-797.
13. CHAK, K.F., CHAO, D.C., TSENG, M.Y., KAO, S.S. AND FENG, T.Y., 1994. Determination and distribution of cry-type genes of Bacillus thuringiensis isolates from Taiwan. Appl. environ. Microbiol, 60:2415-2420.
14. CHANG, J.H., JE, Y.H., ROH, J.Y., PARK, H.W., JIN, B.R., LEE, D.W., KIM, S.H., YANG, Z.W. AND KANG, SR., 1999. Isolation and characterization of a strain of Bacillus thuringiensis serovar kenyae encoding only 5 - endotoxin CrylE. Appl. Ent. Zool, 34:379 -382.
15. CHANG, J.H., ROH, J.Y., JE, Y.H., PARK, H.W., JIN, B.R., WOO, S.D. AND KANG, S.K., 1998. Isolation of a strain of Bacillus thuringiensis ssp. kurstaki HD-1 encoding 5-endotoxin CrylE. Appl. Microbiol, 26: 387-390.
16. CH1LCOTT, C.N. AND WIGLEY, P.J., 1993. Isolation and toxicity of Bacillus thuringiensis from soil and insect habitats in New Zealand. J. Inverteb. Pathol., 61: 244- 247.
17. CHILCOTT, C.N. AND WIGLEY, P.J., 1994. Opportunities for finding new Bacillus thuringiensis strains. Agric. Ecosyst. Environ., 49:51-57.
18. CHUNGJATUPORNCHAI, W.H., HÖFTE, H., SEURINCK, J., ANGSUTHANASOMBAT, C. AND VAECK, M., 1988. Common features of Bacillus thuringiensis toxins specific for Diptera and Lepidoptcra. Europ. J. Biochem., 173:9-16.
19. CRICKMORE, N., ZEIGLER, D.R., FEITELSON, J., SCHNEPF, E., VAN RIE, J., LERECLUS, D., BAUM, J. AND DEAN, D.H., 1998. Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol. Mol. Biol. Rev., 62: 807- 813.
20. DAMGAARD, P.H., 2000. Natural occurrence and dispersal of Bacillus thuringiensis in the environment. In: Enlomopathopgenic bacteria: From laboratory to field applications (eds. J.F. Charles, A. Delecluse and C. Nielson-LeRoux), pp. 23-40. Kluwer Academic Publishers, Dordrecht.
21. DELÉCLUSE, A., BOURGOUINM, C, KLIER, A. AND RAPOPORT, G., 1988. Specificity of action on mosquito larvae of Bacillus thuringiensis israelensis toxins encoded by two different genes. Mol. Gen. Genet., 214: 42-47.
22. DELUCCA, A.J., PALMGREN, M.S. AND C1EGLER, A., 1982. Bacillus thuringiensis in grain elevator dusts. Can. J. Microbiol, 28: 452-456.
23. DELUCCA, A.J., SIMONSON, J.G., AND LARSON, A.D., 1981. Bacillus thuringiensis distribution in soil in the United States. Can. J. Microbiol., 27: 865-870.
24. DONOVAN, W.P, DANKOCSIK, C. AND GILBERT, MP, 1988. Molecular characterization of a gene encoding a 72-kilodalton mosquito-toxic crystal protein from Bacillus thuringiensis subsp. israelensis. J. Bad., 170: 4732-4738.
25. FAUST, R.M., ABE, K., HELD, G.A., IIZUKA, T., BULLA, L.A. AND MEYERS, C.L., 1983. Evidence for plasmid-associated crystal toxin production in Bacillus thuringiensis subsp. israelensis. Plasmid, 9: 98-103.
26. FEDR1CI, B.A., LUTHY, P. AND IBARRA, J.E., 1990. The parasporal body of Bacillus thuringiensis subsp israelensis structure, protein composition and toxicity. In: Bacterial control of mosquitoes and blackflies biochemistry. Genetics and applications of Bacillus thuringiensis and Bacillus sphaericus (cds. H. De Barjac and D.J. Sutherland), pp. 16-44. Rutgers University Press, New Brunswick, NJ, USA.
27. FEITELSON, J.S., PAYNE, J. AND KIM, L., 1992. Bacillus thuringiensis: insects and beyond. J. Biotech., 10: 271- 275.
28. GIFFEL, M.C., BEUMER, RR., KLIJN, N., WAGENDORP, A. AND ROMBOUTS, F.M., 1997. Discrimination between Bacillus cereus and Bacillus thuringiensis using specific DNA probes based on variable regions of I6S rRNA. FEMS. Microbiol. Lett.. 146: 47-51.
29. GOLDBERG, L.J. AND MARGALIT, J., 1977. A bacterial spore demonstrating rapid larvicidal activity against Anopheles segentii, Uranotaenia unguiculata, Culex univitattus, Aedes aegypti and Culex pipiens. Mosquito News, 37: 355-358.
30. GONZALEZ, J.M. JR. AND CARLTON, BC., 1984. A large transmissible plasmid is required for crystal toxin production in Bacillus thuringiensis variety israelensis. Plasmid, 11: 28-38.
31. HEIMPEL, A.M., 1967. A taxonomic key proposed for the species of the cystalliferous bacteria. J. Inverteb. Pathol., 9: 364-373.
32. HEIMPEL, A.M. AND ANGUS, T.A., 1959. The site of action of crystalliferous bacteria in lepidopteran larvae. J. Insect Path., 152-170.
33. HENDERSON, I., DONGZHENG, Y. AND TURNBULL, P.C.B., 1995. Differentiation of Bacillus anthracis and other 'Bacillus cereus group' bacteria using 1S231- derived sequences. F.EM.S. Microbiol. Lett., 128: 113- 118.
34. HÖFTE, H. AND WHITELEY, H.R., 1989. Insecticidal crystal proteins of Bacillus ihuringiensis. Microbiol. Rev., 53: 242-255.
35. IGNOFFO, CM., HOSTETTER, D.L., SIROKOWSKI, P.P., SUTTER, G. AND BROOKS, W.M., 1977. Inactivation of representatives species of anthropathogenic viruses, a bacterium, fungus and protozoan by an ultraviolet light source. Environ. Ent., 6: 411-415.
36. JUA'REZ-PE'REZ, V.M., FERRANDIS, M.D. AND FRUTOS, R., 1997. PCR-based approach for detection of novel Bacillus ihuringiensis cry genes. Appl. environ. Microbiol., 63:2997-3002.
37. KALMAN, S.H., KIEHNE, L., COOPER, N., REYNOSE, M.S. AND YAMAMOTO, T., 1995. Enhanced production of insecticidal proteins in Bacillus ihuringiensis strains carrying an additional crystal protein gene. Appl. environ. Microbiol., 61: 3063-3068.
38. KNOWLES, B.H., 1994. Mechanism of action of Bacillus ihuringiensis insecticidal -5-endotoxins. Adv. Insect. Physiol., 24: 275-308.
39. KRIEG, A., HUGER, A., LANGENBRUCH, G. AND SCHETTER, W., 1983. Bacillus ihuringiensis var. tenebrionis: a new pathotype effective against larvae of Coleoptera. J. appl. Ent., 96: 500-508.
40. LAMBERT, B. AND PEFEROEN, M., 1992. Insecticidal promise of Bacillus thuringiensis. Facts and mysteries about a successful biopesticide. Bioscience, 42: 112- 122.
41. LEE, D.H., SHISA, N., WASANO, N., OHGUSHI, A. AND OHBA, M., 2003. Characterization of flagellar antigens and insecticidal activities of Bacillus thuringiensis populations in animal feces. Curr. Microbiol, 46: 287- 290.
42. LEE, S.G., ECKBLAD, W. AND BULLA, L.A. JR., 1985. Diversity of protein inclusion bodies and identification of mosquitocidal proteins in Bacillus thuringiensis. Biochem. biophys. Res. Commun., 126: 953-960.
43. LIU, CL., MACMULLAN, A., STARNES, R.L., EDWARDS, D.R., KAHN, R. AND MACRAE, T.C., 1991. Abundance, distribution and bio-activities of new Bacillus thuringiensis isolates. First International Conference on Bacillus thuringiensis, St. Catherine's College, Oxford, pp. 28-31.
44. LOWRY, O.H., ROSEBROUGH, N.J., FARR, A.L. AND RANDALL, R.J., 1951. Protein measurement with the Folin phenol reagent. J. biol. Chem., 193: 265-275.
45. MACINTOSH, S.C., STONE, T.B., SIMS, S.R., HUNST, P.L., GREENPLATE, J.T, MARRONE, P.G., PERLAK, F.J., FISCHHOFF, D.A. AND FUCHS, R.L., 1990. Specificity and efficacy of purified Bacillus thuringiensis proteins against agronomically important insects. J. lnverteb. Pathol., 56: 258 -266
46. MAKINO, S., ITO, N., INOUE, T., MIYATA, S. AND MORIYAMA, R., 1994. A spore- lytic enzyme released from Bacillus cereus spore during germination. Microbiology, 140: 1403-1410.
47. MARGALITH, Y. AND BEN-DOV, E., 2000. Biological control by Bacillus thuringiensis subsp. israelensis. In: Insect pest management: techniques for environmental protection (eds. J.E. Rechcigl and N.A. Rechcigl), pp. 243-301. CRC Press, Boca Raton, Fla.
48. MARTIN, P.A.W. AND TRAVERS, R.S., 1989. Worldwide abundance and distribution of Bacillus thuringiensis isolates. Appl. environ. Microbiol., 55: 2437-2442.
49. OBEIDAT, M., HASSAWI, D. AND GHABEISH, I., 2004. Characterization of Bacillus thuringiensis strains from Jordan and their toxicity to the Lepidoptera, Ephestia kuehniella Zeller. African J. Biotech., 3: 622-626.
50. OHBA, M., WASANO, N. AND MIZUKI, E., 2000'. Bacillus thuringiensis soil populations naturally occurring in the Ryukyus, a subtropic region of Japan. Microbiol. Res., 155: 17-22.
51. ORDUZ, S., DIAZ, T., RESTREPO, N., P ATIÑO, M.M. AND TAMAYO, M.C., 1996. Biochemical, immunological and toxicological characteristics of the crystal proteins of Bacillus thuringiensis subsp. medellin. Mem. Inst. Oswaldo. Cruz., 91: 231-237.
52. PAO-INTARA, M., ANGSUTHANASOMBAT, C. AND PANYIM, S., 1988. The mosquito larvicidal activity of 130kDa delta-endotoxin of Bacillus thuringiensis var israelensis resides in the 72kDa amino-terminal fragment. Biochem. biophys. Res. Commun., 153: 294- 300.
53. PARK, H.W., ROH, J.Y., JE, Y.H., JIN, B.R., OH, H.W., PARK. H.Y. AND KANG, S.K., 1998. Isolation of a non-insecticidal Bacillus thuringiensis strain belonging to serotype H8a8b. Lett. appl. Microbiol., 27: 62-66.
54. PETRAS, S.F. AND CASIDA, L,E. JR., 1985. Survival of Bacillus thuringiensis and Bacillus cereus. Z. angew. Ent.. 88:216-221.
55. PRIEST, F.G., KAJI, D.A., ROSATO, Y.B. AND CANHOS VP., 1994. Characterization of Bacillus thuringiensis and related bacteria by ribosomal RNA gene restriction fragment length polymorphisms. Microbiology, 140: 1015-1022.
56. REGIS, L., SILVA-FILHA, M.H., N1ELSEN-LEROUX, C. AND CHARLES, J.F., 2001. Bacterial larvicides of dipteran disease vectors. Trends Parasitol, 17: 377- 380.
57. ROH, J.Y., PARK, H.W., JIN, B.R., KIMJ H.S., YU, Y.M. AND KANG, S.K., 1996. Characterization of novel non-toxic Bacillus thuringiensis isolates from Korea. Lett. appl. Microbiol.. 23:249-252.
58. SAIKI, R.K., GELFAND, D.H., STOFFEL, S., SCHARF, S.J., HIGUCHI, R., HORN, G.T., MULLIS, K.B. AND ERLICH, H.A., 1988. Primer-directed enzymatic amplification of DNA with thermosable DNA polymerase. Science, 239: 487-491.
59. SAYYED, A.H., HAWARD, R., HERRERO, S., FERRE, J. AND WRIGHT, D.J., 2000. Genetic and biochemical approach for characterization of resistance to Bacillus thuringiensis toxin Cry 1 Ac in a field population of the diamondback moth, Plutella xylostella. Appl. environ. Microbiol, 66:1509-1516.
60. SCHNEPF, E., CRICKMORE, N., VAN RIE, J., LERECLUS, D., BAUM, J., FEITELSON, J., ZEIGLER, DR. AND DEAN, D.H., 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Rev., 62: 775-806.
61. SEKI, T., CHUNG, C.K., MIKAMI, H. AND OSHIMA, Y., 1978. Deoxyribonucleic acid homology and taxonomy of the genus Bacillus. Int. J. Syst. Bad., 28: 182-189.
62. SNEATH, P.H.A., 1986. Endospore forming Gram-positive rods and cocci. In: Bergey's manual of systematic bacteriology (ed. J.P. Butler), Williams and Wilkins, Baltimore, vol. I. pp. 1104-1207.
63. THEUNIS, W., AGUDA, R.M., CRUZ, W.T., DECOCK, C., PEFEROEN, M., LAMBERT, B., BOTTRELL, D.G., GOULD, F.L., LITSINGER, J.A. AND COHEN, MB, 1998. Bacillus thuringiensis isolates from the Philippines: habitat distribution, delta -endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae). Bull, entomol. Res., 88:335-342.
64. YAMADA, S., OHASHI, E., AGATA, N. AND VENKATESWARAN, K, 1999. Cloning and nucleotide sequence analysis of gyrB of Bacillus cereus, B. thuringiensis, B. mycoides, and B. anthracis and their application to the detection of B. cereus in rice. Appl. Environ. Microbiol, 6: 1483-1490.