1+1 = 3: A fusion of 2 enzymes in the methionine salvage pathway of Tetrahymena thermophila creates a trifunctional enzyme that catalyzes 3 steps in the pathway

PLoS Genetics

Volumn 5, Issue 10, 2009, Pages

  Salim, Hannah M W ^a,b   Negritto, Maria Cristina ^a   Cavalcanti, Andre R O ^a  

^a POMONA COLLEGE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; METHIONINE; MTNAK PROTEIN; MTNBD PROTEIN; MTNC PROTEIN; UNCLASSIFIED DRUG; PROTOZOAL PROTEIN;

ARTICLE; CATALYSIS; CHEMICAL ANALYSIS; ENZYME ANALYSIS; EUKARYOTE; GENE FUSION; GENETIC CODE; GENETIC COMPLEMENTATION; GENOME; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PROTEIN FUNCTION; TETRAHYMENA THERMOPHILA; YEAST; ANIMAL; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; SACCHAROMYCES CEREVISIAE;

EUKARYOTA; TETRAHYMENA; TETRAHYMENA THERMOPHILA;

ANIMALS; BIOSYNTHETIC PATHWAYS; CATALYSIS; GENE FUSION; METHIONINE; PROTOZOAN PROTEINS; SACCHAROMYCES CEREVISIAE; TETRAHYMENA THERMOPHILA;

EID: 73449103212     PISSN: 15537390     EISSN: 15537404     Source Type: Journal    
DOI: 10.1371/journal.pgen.1000701     Document Type: Article

References (21)

1. Miyazaki J, Yang S (1987) The methionine salvage pathway in relation to ethylene and polyamine biosynthesis. Physiologia Plantarum 69: 366-370.
2. Sufrin JR, Meshnick SR, Spiess AJ, Garofalo-Hannan J, Pan Q, et al. (1995) Methionine recycling pathways and antimalarial drug design. Antimicrobial Agents and Chemotherapy 39(11): 2511-2515.
3. Sekowska A, Danchin A (2002) The methionine salvage pathway in Bacillus subtilis. BMC Microbiol 2: 8.
4. Sekowska A, Denervaud V, Ashida H, Michoud K, Haas D, et al. (2004) Bacterial variations on the methionine salvage pathway. BMC Microbiol 4: 9.
5. Ashida H, Saito Y, Kojima C, Yokota A (2008) Enzymatic Characterization of 5-Methylthioribulose-1-phosphate Dehydratase of the Methionine Salvage Pathway in Bacillus subtilis. Biosci Biotechnol Biochem 72(4): 959-967.
6. Yang SF, Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Ann Rev Plant Phys 35: 155-189.
7. Cellarier E, Durando X, Vasson MP, Farges MC, Demiden A, et al. (2003) Methionine dependency and cancer treatment. Tumour Rev 29(6): 489-499.
8. Rosado J, Salvador M, Bonatto D (2007) Importance of the trans-sulfuration pathway in cancer prvention and promotion. Mol Cell Biochem 301: 1-12.
9. Pirkov I, Norbeck J, Gustafsson L, Albers E (2008) A complete inventory of all enzymes in the eukaryotic methionine salvage pathway. FEBS J 275: 4111-4120.
10. Thomas D, Becker A, Surdin-Kerjan Y (2000) Reverse methionine biosynthesis from S-adenosylmethionine in eukaryotic cells. J Biol Chem 275: 40718-40724.
11. Galperin MY, Koonin EV (2000) Who's your neighbor? New computational approaches for functional genomics. Nat Biotechnol 18: 609-613.
12. Veitia RA (2002) Rosetta Stone proteins: "chance and necessity"? Genome Biol 3: interactions 1001.1-1001.3.
13. Stechmann A, Cavalier-Smith T (2002) Rooting the Eukaryote Tree by Using a Derived Gene Fusion. Science 297: 89-91.
14. Stechmann A, Cavalier-Smith T (2003) The root of the eukaryote tree pinpointed. Curr Biol 13: R665-R666.
15. Nara T, Hshimoto T, Aoki T (2000) Evolutionary implications of the mosaic pyrimidine-biosynthetic pathway in eukaryotes. Gene 257: 209-222.
16. Dai Y, Wensink PC, Abeles RH (1999) One protein, two enzymes. J Biol Chem 274: 1193-1195.
17. Xu Q, Schwarzenbacher R, Krishna SS, McMullan D, Agarwalla S, et al. (2006) Crystal structure of acireductone dioxygenase (ARD) from Mus musculus at 2.06 A resolution. Proteins 64: 808-813.
18. Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, et al. (2008) KEGG for linking genomes to life and the environment. Nucleic Acids Res 36: D480-D484.
19. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389-3402.
20. Pruitt KD, Tatusova T, Maglott DR (2007) NCBI Reference Sequence (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins. Nucleic Acids Res 35: D61-D65.
21. Horowitz S, Gorovsky MA (1985) An unusual genetic code in nuclear genes of Tetrahymena. PNAS 82: 2452-2455.