Isolating aptamers using capillary electrophoresis-SELEX (CE-SELEX)

Methods in Molecular Biology

Volumn 535, Issue , 2009, Pages 33-43

  Mosing, Renee K ^a   Bowser, Michael T ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

aptamers; capillary electrophoresis; CE SELEX; in vitro evolution; in vitro selection; SELEX

Indexed keywords

ALCOHOL; APTAMER; LIGAND; STREPTAVIDIN;

ARTICLE; CAPILLARY ELECTROPHORESIS; CHEMISTRY; COMBINATORIAL CHEMISTRY; ECONOMICS; GEL CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYMERASE CHAIN REACTION; PRECIPITATION; TIME;

APTAMERS, NUCLEOTIDE; CHEMICAL PRECIPITATION; CHROMATOGRAPHY, AGAROSE; ELECTROPHORESIS, CAPILLARY; ETHANOL; LIGANDS; POLYMERASE CHAIN REACTION; SELEX APTAMER TECHNIQUE; STREPTAVIDIN; TIME FACTORS;

EID: 73449102272     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-557-2_3     Document Type: Article

References (10)

1. Joyce, G.F. (1989) Amplification, mutation, and selection of catalytic RNA. Gene 82, 83-87. (Pubitemid 19248661)
2. Ellington, A. D. and Szostak, J. W. (1990) In vitro selection of RNA molecules that bind specific ligands. Nature 346, 818-822.
3. Tuerk, C. and Gold, L. (1990) Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249, 505-510.
4. Famulok, M., Mayer G., and Blind, M. (2000) Nucleic acid aptamers-from selection in vitro to applications in vivo. Acc. Chem. Res. 33, 591-599.
5. Hamula, C.L.A., Guthrie, J.W., Zhang, H., Li, X.-F. and Le, X. C. (2006) Selection and analytical applications of aptamers. Trends. Analyt. Chem. 25, 681-691. (Pubitemid 44109900)
6. Bunka, D.H.J. and Stockley, P.G. (2006) Aptamers come of age-at last. Natl. Rev. Microbiol. 4, 588-96.
7. Tombelli, S., Minunni, M. and Mascini, M. (2005) Analytical applications of aptamers. Biosens. Bioelectron. 20, 2424-2434. (Pubitemid 40602182)
8. Mendonsa, S.D. and Bowser, M.T. (2004) In vitro evolution of functional DNA using capillary electrophoresis. J. Am. Chem. Soc. 126, 20-21. (Pubitemid 38090319)
9. Mendonsa, S.D. and Bowser, M.T. (2004) In vitro selection of high-affinity DNA ligands for human IgE using capillary elec-trophoresis. Anal. Chem. 76, 5387-5392. (Pubitemid 39249420)
10. Mosing, R.K., Mendonsa, S.D. and Bowser, M.T. (2005) Capillary electrophoresis-SELEX selection of aptamers with affinity for HIV-1 reverse transcriptase. Anal. Chem., 77, 6107-6112. (Pubitemid 41436941)