Purification and characterization of an extracellular levansucrase from Zymomonas mobilis ZM1 (ATCC 10988)

Korean Journal of Applied Microbiology and Biotechnology

Volumn 26, Issue 4, 1998, Pages 309-315

  Song, Ki Bang ^a   Seo, Jeong Woo ^a   Joo, Hyun Kyu ^b   Rhee, Sang Ki ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b SunMoon University   (South Korea)

Author keywords

Levan; Levansucrase; Zymomonas mobilis

Indexed keywords

LEVANSUCRASE; TRITON X 100;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; HYDROLYSIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; ZYMOMONAS MOBILIS;

EID: 7344255752     PISSN: 02572389     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Belghith, H., K. B. Song, C. H. Kim, and S. K. Rhee. 1996. Optimal conditions for levan formation by an overexpressed recombinant levansucrase. Biotechnol. Lett. 18: 467-472.
2. Biton, J., J. M. Michel, D. Le Beller, V. Pelenc, F. Paul, P. Monsan, and G. Gellf. 1995. Enzymatic synthesis of low-carolie sugar substitutes. Ann. N.Y. Acad. Sci. 750: 321-324.
3. Cheetham, P. S. J., A. J. Hacking, and M. Vlitos. 1989. Synthesis of novel disaccharides by a newly isolated fructosyl transferase from Bacillus subtilis. Enzyme Microb. Technol. 11: 212-219.
4. Chung, B. H., W. K. Kim, K. B. Song, C. H. Kim, and S. K. Rhee. 1997. Novel polyethylene glycol/levan aqueous two-phase system for protein partitioning. Biotechnol. Techniques 11: 327-329.
5. Doelle, H. W. and P. F. Greenfield. 1985. Fermentation pattern of Zymomonas mobilis at high sucrose concentration. Appl. Microbiol. Biotechnol. 22: 411-416.
6. Elisashvili, V. I. 1984. Levan synthesis by Bacillus sp. Appl. Biochem. Microbiol. 20: 82-87.
7. Gunasekaran, P., T. Karunakaran, and J. Baratti. 1990. Cloning and sequencing of the sacA gene: Characterization of a sucrase from Zymomonas mobilis. J. Bacteriol. 172: 6727-6735.
8. Han, Y. W. and M. A. Clarke. 1990. Microbial fructan, production and characterization, Chapter 18. Agricultural and Synthetic Polymers, American Chemical Society, New York.
9. Hettwer, U, M. Gross, and K. Rudolph. 1995. Purification and characterization of an extracellular levansucrase from Pseudomonas syringae pv. phaseolicola. J. Bacteriol. 177: 2834-2839.
10. Johns, M. R., P. F. Greenfield, and H. W. Doelle. 1991. Byproducts from Zymomonas mobilis, pp. 97-121. In A. Fiechter(ed.) Advances in Biochem. Eng. Biotech., Vol. 44, Springer-Verlag, Berlin.
11. Laemmli, U. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature (London) 227: 680-685.
12. Leibovich, J. and Y. Stark. 1985. Increase of permeability to a cytotoxic agent by the polysaccharide levan. Cell. Mol. Biol. 31: 337-341.
13. Lyness, E. W. and H. W. Doelle. 1983. Levansucrase from Zymomonas mobilis. Biotechnol. Lett. 5: 345-350.
14. Ohtsuka, K., S. Hino, T. Fukushima, O. Ozawa, T. Kanematsu, and T. Uchida. 1992. Characterization of levansucrase from Rahnella aquatilis JCM-1683. Biosci. Biotech. Biochem. 56: 1373-1377.
15. O'Mullan, P., M. Szakucs-Dobozi, and D. E. Eveleigh. 1991. Identification of saccharolytic enzymes of Zymomonas mobilis CP4. Biotechnol. Lett. 13: 137-142.
16. Park, Y. K., M. P. L. Mortatti, and H. H. Sato.1983. Study on levan formation during fermentation of Zymomonas mobilis on sucrose. Appl. Eur. Microbiol. 44: 515-518.
17. Perez Oseguera M. A., L. Guereca, and A. Lopez-Munguia. 1996. Properties of levansucrase from Bacillus circulans. Appl. Microbiol. Biotechnol. 45: 465-471.
18. Petit-Glatron, M. F., R. Chambert, and M. Steinmetz. 1980. Levansucrase of Bacillus subtilis: Characterization of a form isolated from phenol-treated cells and activated by Triton X-100. Eur. J. Biochem. 103: 189-193.
19. Romano, A. H. 1986. Microbial sugar transport systems and their importance in biotechnology. Trends Biotechnol. 4: 207-213.
20. Skotnicki, M. L., K. J. Lee, K. J. Tribe, and P. L. Rogers. 1982. Genetic alteration of Zymomonas mobilis for ethanol production, pp. 271-290. In A. Hollaender(ed.), Genetic Engineering of Microorganisms for Chemicals, Plenum Press.
21. Song, K. B., H. Belghith, and S. K. Rhee. 1996. Production of levan, a fructose polymer, using an overexpressed recombinant levansucrases. Ann. N.Y. Acad. Sci 799: 601 -607.
22. Song, K. B., H. K. Joo, and S. K. Rhee. 1993. Nucleotide sequence of levansucrase gene (levU) of Zymomonas mobilis ZM1 (ATCC 10988). Biochim. Biophys. Acta. 1173: 320-324.
23. Song, K. B., S. K. Lee, H. K. Joo, and S. K. Rhee. 1994. Nucleotide and derived amino acid sequences of an extracellular sucrase gene (invB) of Zymomonas mobilis ZM1(ATCC 10988). Biochim. Biophys. Acta. 1219: 163-166.
24. Swings, J. and J. De Ley. 1977. The biology of Zymomonas. Bacteriol. Rev. Mar: 1-46.
25. Tanaka, T., T. Karigane, S. Fuji, T. Chinzaka, and S. Nakamura. 1985. Intermolecular fructosyl and levanbiosyl transfers by levan fructotransferase of Arthrobacter ureafaciens. J. Biochem. 97: 1679-1688.
26. Viikari, L. 1988. Carbohydrate metabolism in Zymomonas. CRC Crit. Rev. Biotechnol. 7: 237-261.
27. Yanase, H., M. Iwata, R. Nakahigashi, K. Kita, N. Kato, and K. Tonomura. 1992. Purification, crystallization, and properties of the extracellular levansucrase from Zymomonas mobilis. Biosci. Biotech. Biochem. 56: 1335-1337.